Abstract:
:C-type natriuretic peptide (CNP) was recently found in the myocardium, but possible insights into differences between atrium and ventricle production are so far lacking. Our aim was to evaluate, in an experimental model of pacing-induced heart failure (HF), plasma and tissue levels of CNP and mRNA expression of the peptide and of its specific receptor, NPR-B. Cardiac tissue was collected from male adult minipigs without (control, n=5) and with pacing-induced HF (n=5). Blood samples were collected at baseline and after pacing (10 min, 1, 2, 3 weeks). CNP in plasma and in cardiac extracts was determined by a radioimmunoassay, while the expression of mRNA by real time PCR. Compared to control, plasma CNP was increased after 1 week of pacing stress (36.9+/-10.4 pg/ml vs.16.7+/-1.1, p=0.013, mean+/-S.E.M.). As to myocardial extract, at baseline, CNP was found in all cardiac chambers and its content was 10-fold higher in atria than in ventricles (RA: 13.7+/-1.9 pg/mg protein; LA: 8.7+/-3.8; RV: 1.07+/-0.33; LV: 0.93+/-0.17). At 3 weeks of pacing, myocardial levels of CNP in left ventricle were higher than in controls (15.8+/-9.9 pg/mg protein vs. 0.9+/-0.17, p=0.01). CNP gene expression was observed in controls and at 3 weeks of pacing. NPR-B gene expression was found in all cardiac regions analyzed, and a down-regulation was observed in ventricles after HF. The co-localization of the CNP system and NPR-B suggests a possible role of CNP in HF and may prompt novel therapeutical strategies.
journal_name
Peptidesjournal_title
Peptidesauthors
Del Ry S,Cabiati M,Lionetti V,Emdin M,Recchia FA,Giannessi Ddoi
10.1016/j.peptides.2008.09.005subject
Has Abstractpub_date
2008-12-01 00:00:00pages
2208-15issue
12eissn
0196-9781issn
1873-5169pii
S0196-9781(08)00374-4journal_volume
29pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::Venom small peptides that target neurotrophin receptors might be beneficial in neurodegeneration, including Parkinsońs disease (PD). Their small size, ease of synthesis, structural stability and target selectivity make them important tools to overcome the limitations of endogenous neurotrophins as therapeutic agents. ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2018.04.012
更新日期:2018-06-01 00:00:00
abstract::In the Nucleus Ambiguus (NA) mu-receptors increased mean arterial pressure (MAP) and heart rate (HR) and kappa-receptors decreased MAP without altering HR. Mu-receptors in the Dorsal Motor Nucleus of the Vagus (DMV) increased MAP without changing HR whereas kappa-receptors in the DMV decreased HR without changing MAP....
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(88)90225-2
更新日期:1988-01-01 00:00:00
abstract::Pharmacological study of Phe-Met-Leu-Phe-amide (FMRFa) receptors is hindered by the lack of selective ligands. The classification of these selective ligands is further hampered by the limited availability of functional assays. In this study, we evaluated several synthetic FMRFa analogs for agonist and antagonist activ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(97)00481-6
更新日期:1998-01-01 00:00:00
abstract::In the present review we reevaluated the experimental and clinical evidence that there is a local renin angiotensin system in the heart as well as the presence of a functional intracrine component which is activated during pathological conditions like heart failure and hypertension. The implications of these findings ...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2011.06.018
更新日期:2011-08-01 00:00:00
abstract::The striatum is richly innervated by both enkephalinergic and dopaminergic neurons, providing an anatomic framework from which intimate functional interrelationships between these neuronal systems may be postulated. Accordingly, many functional processes within dopamine neurons have been shown to be modulated by opioi...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(87)90014-3
更新日期:1987-05-01 00:00:00
abstract::Purified mast cell carboxypeptidase cleaved the C-terminal leucines from Leu5-enkephalin (Leu-ENK), neurotensin (NT), and kinetensin (KT), with Km values of 36, 16, and 15 microM, and kcat values of 44, 51, and 53 s-1, respectively. To better predict potential in vivo hydrolysis products generated by mast cell proteas...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(91)90049-u
更新日期:1991-09-01 00:00:00
abstract::Jingzhaotoxin-II (JZTX-II) is a 32-residue peptide from the Chinese tarantula Chilobrachys jingzhao venom, and preferentially inhibits the fast inactivation of the voltage-gated sodium channels (VGSCs) in rat cardiac myocytes. In the present study, we elucidated the action mechanism of JZTX-II inhibiting hNav1.5, a VG...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2015.03.012
更新日期:2015-06-01 00:00:00
abstract::Injection of capsaicin into the hindpaw has been employed as a model of chemogenic nociception in mice. Intraplantar injection of nociceptin (30-240 pmol) produced a significant and dose-dependent antinociceptive activity in the capsaicin test. The nociceptin N-terminal fragments, (1-11) and (1-13), were also active w...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.05.022
更新日期:2005-12-01 00:00:00
abstract::The cholecystokinin receptor-type 1 (CCK1R) is a G-protein coupled receptor localized in the animal gastrointestinal tract. Receptor activation by the natural peptide ligand CCK leads to a feeling of satiety. In this study, hydrolysates from soy and milk proteins were evaluated for their potential to activate the CCK1...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.11.019
更新日期:2012-03-01 00:00:00
abstract::Chemerin is a novel peptide that was identified as a natural ligand for ChemR23. As it has been reported to be involved in the regulation of immune responses and adipogenesis, chemerin may have a variety of physiological functions. Chemerin is synthesized as a precursor (prochemerin) and is proteolytically activated a...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.05.030
更新日期:2009-08-01 00:00:00
abstract::Enzyme-resistant receptor agonists of the incretin hormone glucagon-like peptide-1 (GLP-1) have shown positive therapeutic effects in people with type 2 diabetes mellitus (T2DM). T2DM has detrimental effects on brain function and impairment of cognition and memory formation has been described. One of the underlying me...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2017.11.017
更新日期:2018-02-01 00:00:00
abstract::The objective of this study was to explore the effects of the endogenous opioid peptide dynorphin A(1-13) on the CNS regulation of blood pressure and heart rate. Wistar rats, anesthetized with pentobarbital and halothane, received dynorphin A(1-13) microinjected into the anterior hypothalamus area (AHA), the posterior...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90184-i
更新日期:1993-11-01 00:00:00
abstract::Neurotensin(6-13) has been isolated and sequenced as the major form of neurotensin-like immunoreactivity (NTLI) in a human hepatic fibrolamellar carcinoma. Circulating NTLI in the patient, especially C-terminal, was very high. In additional studies, NT(6-13) was synthesized and compared with the purified tumor NTLI by...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(91)90152-f
更新日期:1991-07-01 00:00:00
abstract::This study was designed to test the possibility that antimicrobial peptides could be derived from the genomic sequences of phage lysins. Using two lysins (D3 and PhiKZ) we selected and produced two putative peptides (X and Z, respectively) believed to possess antimicrobial properties based on their physicochemical cha...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.07.001
更新日期:2006-01-01 00:00:00
abstract::ZP120 is a nociceptin/orphanin FQ (N/OFQ) peptide receptor (NOP) ligand. In previous studies, the effects of ZP120 were found to be sensitive to J-113397 in mouse tissues while resistant to UFP-101 in rat tissues. The aim of this study was to further investigate the ZP120 pharmacological profile using mouse and rat pr...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2008.10.001
更新日期:2009-02-01 00:00:00
abstract::Circulating urotensin II (UII) concentrations and the tissue expression of its cognate receptor (UT) are elevated in patients with cardiovascular disease (CVD). The functional significance of elevated plasma UII levels in CVD is unclear. Urotensin-related peptide (URP) is a paralog of UII in that it contains the six a...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2007.08.013
更新日期:2008-05-01 00:00:00
abstract::Vasoactive intestinal peptide (VIP) is a neuropeptide present in high concentrations in the hippocampus. The studies reported here demonstrate that VIP administered into the third ventricle of the brain caused amnesia in mice trained on a left-right footshock avoidance task in a T-maze. VIP resulted in amnesia when ad...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(90)90012-t
更新日期:1990-09-01 00:00:00
abstract::The monoclonal antibody 2F5 recognizing the neutralizing epitope ELDKWA on the C-domain could neutralize 90% of the investigated HIV-1 isolates. Low levels of ELDKWA-epitope-specific antibodies were observed in HIV-1-infected individuals. To induce high levels of antibodies to ELDKW-epitope, C-domain peptide (P2) was ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(00)00179-0
更新日期:2000-04-01 00:00:00
abstract::To identify male-specific genes that could be involved in male development, we screened a subtracted male reproductive tract library and isolated a novel gene named Mar-Mrr (M. rosenbergii male reproduction-related gene). The Mar-Mrr cDNA sequence consists of 683 nucleotides with a 333 nucleotide open reading frame, e...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.09.004
更新日期:2006-04-01 00:00:00
abstract::Two peptides, rHRnG and hproHRnG, which were encoded by the nucleotide sequences complementary to mRNA of rat hypothalamic gonadotropin-releasing hormone (GnRH) and human placental proGnRH(-3-13), respectively, were synthesized. A remarkable hydropathic anti-complementarity was observed in the N-terminal region betwee...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(86)90144-0
更新日期:1986-11-01 00:00:00
abstract::Crude extracts of the marine sponge Geodia corticostylifera from Brazilian Coast have previously shown antibacterial, antifungal, cytotoxic, haemolytic and neurotoxic activities. The present work describes the isolation of the cyclic peptides geodiamolides A, B, H and I (1-4) from G. corticostylifera and their anti-pr...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2006.04.021
更新日期:2006-09-01 00:00:00
abstract::Antisauvagine-30 (aSVG) is the only high-affinity antagonist for the corticotropin-releasing factor (CRF) type 2 (CRF(2)) receptor. A structure-activity relationship study was performed to pinpoint residues conferring aSVG's selectivity. The aSVG-analogues being N-terminally extended by one or two residues or containi...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(02)00139-0
更新日期:2002-10-01 00:00:00
abstract::The effect of peripheral injections of substance P (SP) on performance in two different configurations of an automated tunnel maze was examined in three experiments. In two experiments, the effect of pretrial SP injections (10-1000 micrograms/kg) on performance in the hexagonal and radial maze configuration of an auto...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90014-8
更新日期:1993-01-01 00:00:00
abstract::In the present study, the newly synthesized TRH analog (L-pGlu-(2-propyl)-L-His-l-ProNH(2); NP-647) was evaluated for its effects in in vitro (oxygen glucose deprivation (OGD)-, glutamate- and H(2)O(2)-induced injury in PC-12 cells) and in vivo (transient global ischemia) models of cerebral ischemic injury. PC-12 cell...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.04.006
更新日期:2011-06-01 00:00:00
abstract::Lysine-8-vasopressin (LVP) was injected into the lateral ventricle (ICV) or into the cisterna magna (c.m.) of ether-anesthetized rats. ICV injection of LVP decreased the blood pressure (BP), whereas c.m. administration increased it. The opposite effects of ICV versus c.m. administration of the peptide might be related...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(86)90021-5
更新日期:1986-05-01 00:00:00
abstract::The actions of a behaviorally active luteinizing hormone-releasing hormone fragment, Ac-LHRH(5-10), on CA1 pyramidal cells were studied utilizing conventional intracellular recordings from the in vitro rat hippocampal slice preparation. The behaviorally active fragment (10(-7) M) and the natural decapeptide [LHRH(1-10...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90090-4
更新日期:1993-09-01 00:00:00
abstract::To investigate the contribution of the N-terminal sequence of h-EGF to its biological activity and the formation of three intramolecular disulfide bonds by oxidative refolding via air oxidation, five derivatives of h-EGF with a single N-terminal amino acid deletion were synthesized by solid-phase synthesis. The homoge...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)00181-2
更新日期:1995-01-01 00:00:00
abstract::Male Holtzman albino and Long-Evans hooded rats were administered one microgram of arginine vasopressin (AVP) or a placebo each day after the acquisition trials of a visual white-black discrimination. Animals were then trained in the reversal of the discrimination. Performance was assessed by the number of trials to c...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(89)90099-5
更新日期:1989-01-01 00:00:00
abstract::The major active product of ghrelin gene is a 28-amino acid peptide acylated at the serine 3 position with an octanoyl group, called simply ghrelin. Ghrelin has a multiplicity of physiological functions, affecting GH release, food intake, energy and glucose homeostasis, gastrointestinal, cardiovascular, pulmonary and ...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2008.02.018
更新日期:2008-07-01 00:00:00
abstract::The aim of the present study was to investigate how suckling influences the plasma levels of oxytocin and vasoactive intestinal peptide in lactating dogs. Blood-samples were drawn from ten lactating beagles during suckling, in week one and week three of the five week long lactation period and the levels of vasoactive ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(87)90002-7
更新日期:1987-05-01 00:00:00