Properties of a synaptic vesicle protein binding plasma membranes.

Abstract:

:Synaptic vesicles contain a protein (agglutinin) which binds cell plasma membranes. The protein activity is titrated measuring the agglutination of rabbit trypsinized-fixed red blood cells induced by serial dilutions of purified vesicles. The protein is highly concentrated in vesicles: the specific activity is 150-fold higher in vesicles compared to the brain homogenate, and 20-fold higher compared to the crude synaptosomal membrane fraction. The binding is specifically inhibited by the four major brain sialoglycosphingolipids, with a higher affinity for polysialo- rather than for monosialoganglioside (GM1) IC50 are 3.9 x 10(-5) M (GT1b), 8.9 x 10(-5) M (GD1a), 2.8 x 10(-4) M (GD1b) and 4.9 x 10(-4) M (GM1). Cells which are not agglutinated by the vesicles (human red blood cells) can be activated by incubation with gangliosides: insertion of the glycolipids in the plasma membranes makes them sensitive to the vesicle haemagglutinin.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Popoli M,Paterno R

doi

10.1097/00001756-199102000-00007

subject

Has Abstract

pub_date

1991-02-01 00:00:00

pages

93-5

issue

2

eissn

0959-4965

issn

1473-558X

journal_volume

2

pub_type

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