Abstract：
PURPOSE:To compare polymerase chain reaction (PCR) to microbial culture for the detection and identification of bacterial and fungal pathogens in microbial keratitis. DESIGN:Prospective cohort study. METHODS:A total of 108 consecutive corneal ulcers were cultured and analyzed by PCR using pan-bacterial and pan-fungal primers. PCR products were cloned, sequenced, and compared to culture results using standard bioinformatics tools. RESULTS:Of the 108 samples, 56 were culture-positive, 25 for bacteria and 31 for fungi; 52 were culture-negative. After eliminating false-positive PCR products, 94 of 108 were positive by PCR, 37 for bacteria and 57 for fungi. Nineteen of 25 bacterial culture-positive samples were positive by PCR, and 29 of 31 samples culture-positive for fungi were positive by PCR. The majority of sequenced PCR products matched the positive culture results. Of the 52 culture-negative samples, 46 (88%) yielded pathogen deoxyribonucleic acid (DNA) PCR products, 18 bacterial and 28 fungal. These represented a variety of species, including at least three novel previously uncultured microbes. CONCLUSIONS:PCR detects microbial DNA in the majority of bacterial and fungal corneal ulcers, and identifies potentially pathogenic organisms in a high proportion of culture-negative cases. Yield and concordance with culture are higher for fungal than bacterial ulcers. Practical use of the technique is limited by artefactual amplification of nonpathogenic organisms. PCR may be used as an adjunct to culture to identify potential pathogens in microbial keratitis.
journal_name
Am J Ophthalmoljournal_title
American journal of ophthalmologyauthors
Kim E,Chidambaram JD,Srinivasan M,Lalitha P,Wee D,Lietman TM,Whitcher JP,Van Gelder RNdoi
10.1016/j.ajo.2008.06.009subject
Has Abstractpub_date
2008-11-01 00:00:00pages
714-23, 723.e1issue
5eissn
0002-9394issn
1879-1891pii
S0002-9394(08)00473-Xjournal_volume
146pub_type
杂志文章abstract：PURPOSE:To investigate the magnitude, with-the-rule (WTR) or against-the-rule (ATR) orientation, and vector components (Jackson astigmatic vectors [J0 and J45] and blurring strength) of the anterior and posterior corneal astigmatism (ACA and PCA) in patients with keratoconus (KC) in a retrospective study, and to try to...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
pub_type： 杂志文章,多中心研究,随机对照试验
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更新日期：2019-08-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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更新日期：2009-07-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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更新日期：2014-11-01 00:00:00
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journal_title：American journal of ophthalmology
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更新日期：2004-04-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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更新日期：1979-09-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2003.10.034
更新日期：2004-01-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(75)90089-6
更新日期：1975-02-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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journal_title：American journal of ophthalmology
pub_type： 杂志文章,评审
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
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更新日期：2003-02-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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更新日期：1987-04-15 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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更新日期：1987-11-15 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章,评审
doi：10.1016/s0002-9394(14)72168-3
更新日期：1995-09-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(14)72616-9
更新日期：1995-08-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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更新日期：1991-07-15 00:00:00
abstract：PURPOSE:To assess a response to infliximab therapy in childhood uveitis. DESIGN:Retrospective case series. METHODS:We reviewed the course of 16 children with noninfectious uveitis treated with infliximab at an academic medical center. Outcome measures included incidence of uveitis recurrences, proportion of patients ...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
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更新日期：2007-12-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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更新日期：2008-04-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章,随机对照试验
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更新日期：2020-12-01 00:00:00
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journal_title：American journal of ophthalmology
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更新日期：2016-12-01 00:00:00
