Abstract：
:Survivin is known to be overexpressed in various human malignancies, including pancreatic cancer, and to cause resistance to radiation and chemotherapy, so the regulation of this molecule could be a new strategy for treating pancreatic cancer. In our study, a short interfering RNA (siRNA) plasmid expression vector against survivin was constructed and transfected into human pancreatic cancer cell lines of Panc-1 and BxPC3. The expression of survivin mRNA and protein among the stable transfected cells and the untransfected cells was detected by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot, respectively. Tumor cell growth in vitro was assessed by trypan blue exclusion. The cell cycle distribution and cell apoptosis were measured by flow cytometry. The cytotoxicity assay was measured by the MTT test. Our results showed that survivin siRNA treatment caused a specific and profound decrease of survivin mRNA and protein that was associated with decreased cell growth, spontaneous apoptosis, and a specific G0/G1 arrest. Furthermore, the suppression of survivin can enhance the chemosensitivity of pancreatic cancer cells to gemcitabine significantly. We suggest that the RNAi against survivin gene strategy would be a potential approach to chemosensitization therapy in human pancreatic cancer.
journal_name
Dig Dis Scijournal_title
Digestive diseases and sciencesauthors
Liu WS,Yan HJ,Qin RY,Tian R,Wang M,Jiang JX,Shen M,Shi CJdoi
10.1007/s10620-008-0329-4subject
Has Abstractpub_date
2009-01-01 00:00:00pages
89-96issue
1eissn
0163-2116issn
1573-2568journal_volume
54pub_type
杂志文章abstract：:The present study investigated how reflux-induced esophagitis affects the smooth muscle reactivity of the esophageal body. Two different esophagitis models were used: acid gastric and mixed duodenogastric reflux. All test animals apart from the controls developed gross and histologic evidence of esophagitis. Contracti...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/b:ddas.0000004518.98825.6c
更新日期：2003-11-01 00:00:00
abstract：:Exposure of the stomach for 30 min to acidified sodium taurocholate (TC) (1-20 mM) or sodium salicylate (SA) (10-80 mM) caused a reduction of transmucosal PD and an increase of luminal pH in anesthetized rats, in a concentration-related manner. Acidified aspirin (ASA) (10-80 mM) reduced PD in the same manner, without ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01296714
更新日期：1987-08-01 00:00:00
abstract：:Excessive spontaneous swallowing has been associated with a variety of common gastrointestinal symptoms including abdominal pain, heartburn, and bloating and may contribute to disorders such as hiatus hernia, duodenal ulcer, and irritable bowel syndrome. The present study investigated the hypothesis that changes in em...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02065410
更新日期：1995-02-01 00:00:00
abstract：BACKGROUND:Barrett's esophagus (BE) is characterized by a distinct Th2-predominant cytokine profile. However, antigens that shift the immune response toward the Th2 profile are unknown. AIM:We examined the effects of rebamipide on the esophageal microbiome and BE development in a rat model. METHODS:BE was induced by ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-015-3662-4
更新日期：2015-09-01 00:00:00
abstract：BACKGROUND AND AIM:The purpose of this study was to examine the association between celiac disease (CD) and prior respiratory syncytial virus (RSV) infection or any viral bronchiolitis. METHODS:This was a retrospective case-control study. During 2006-2008 small intestinal biopsy data were collected from Sweden's 28 pa...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-014-3046-1
更新日期：2014-07-01 00:00:00
abstract：AIM:Renal toxicity of adefovir disoproxil (ADV) and tenofovir disoproxil fumarate (TDF) is a significant concern in chronic hepatitis B (CHB) patients. Early observational clinical data suggested that telbivudine (LdT) might have renoprotective effects. METHODS:In this prospective study, consecutive CHB patients on co...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-019-05717-0
更新日期：2019-12-01 00:00:00
abstract：:Low or minimal doses of aspirin are widely used for prevention of cardiovascular diseases. Aspirin is known to produce severe adverse gastrointestinal effects, such as bleeding and perforation. Less is known about the risk associated with minidose aspirin. Our aim was to assess the possible association of upper gastro...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-005-2906-0
更新日期：2005-09-01 00:00:00
abstract：:To test the role of immune reactivity in the pathogenesis of hepatitis C, serum soluble immune factors were measured in a cohort of 57 patients with chronic hepatitis C, and in 20 healthy subjects. Levels of interleukin-1 beta, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor-alpha, and interleu...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02087671
更新日期：1994-11-01 00:00:00
abstract：:Fibronectin (FN) is important for wound healing via cell proliferation, adhesion, differentiation, and matrix formation. Fetal types of FN mRNA, which include the region of EIIIA or EIIIB, are well expressed during embryonic development and wound healing. This study was done to investigate the mRNA expression of full-...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1005541305255
更新日期：2001-02-01 00:00:00
abstract：:Despite many studies on pain of functional gastrointestinal disorders (FGID), the pain mechanism of FGID is not well understood, and pain treatment of FGID is not established. Following our former functional dyspepsia (FD) study, we proposed two subgroups of patients with irritable bowel syndrome (IBS), pain and disco...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/b:ddas.0000026301.47638.92
更新日期：2004-04-01 00:00:00
abstract：:There are no requirements concerning the amplitude of simultaneous contractions among the present criteria for the manometric diagnosis of diffuse esophageal spasm. The purpose of this investigation was to determine whether the current criteria effectively identify an appropriately homogenous patient population. Sixty...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02088558
更新日期：1996-07-01 00:00:00
abstract：:A gangliocytic paraganglioma (GP) is an extremely rare neurogenic tumor nearly located in the second portion of the duodenum and has been regarded as benign. We report a case of duodenal GP in a 53-year-old man. The GP arose in the second portion of the duodenum and was shown by histological examination to consist of ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-006-9370-3
更新日期：2007-06-01 00:00:00
abstract：INTRODUCTION:Assessment of whole gut transit, by radio-opaque markers or scintigraphy, is used to evaluate patients with constipation for slow gastrointestinal transit. Wireless capsule motility, using the SmartPill GI monitoring system, samples and transmits intraluminal pH, pressure, and temperature data from a capsu...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-009-0899-9
更新日期：2009-10-01 00:00:00
abstract：:There is A paucity of data on racial differences in epidemiology of irritable bowel syndrome (IBS) alone and un-investigated dyspepsia (UD) alone compared to "overlap syndrome" (OS). We conducted a random survey (n = 990). Subjects completed a questionnaire which included Rome II criteria for IBS and functional dyspep...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-006-3112-4
更新日期：2006-01-01 00:00:00
abstract：:Longitudinal shortening of the esophagus during peristaltic contraction has been previously analyzed globally using spaced mucosal clips. This method gives a relatively crude measurement. In this study, local longitudinal shortening (LLS) was evaluated using simultaneous high-resolution endoluminal ultrasound (HREUS) ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-006-3092-4
更新日期：2006-01-01 00:00:00
abstract：:Ehlers-Danlos syndrome (EDS) type IV is an autosomal dominant connective tissue disease caused by mutations in the type III collagen gene resulting in extreme tissue fragility. Affected individuals are at risk of dramatic and often fatal complications, mostly spontaneous arterial, uterine, or colonic ruptures. Phenoty...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1018869617076
更新日期：1997-08-01 00:00:00
abstract：BACKGROUND/AIM:Familial Mediterranean fever (FMF), the most frequent periodic fever syndrome, is an autosomal recessive inherited disease that predominantly affects eastern Mediterranean populations. Fetuin-A is a well known negative acute-phase protein. Studies of this glycoprotein as a marker of inflammation in FMF a...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-013-2814-7
更新日期：2013-11-01 00:00:00
abstract：:Gallbladder contractility has been studied in 21 obese patients (greater than 130% ideal weight) and 30 nonobese subjects before and at regular intervals after the administration of a regular solid-liquid meal, and after a low-calorie, low-fat meal used conventionally for weight-loss purposes (Modifast). Gallbladder v...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01536623
更新日期：1988-01-01 00:00:00
abstract：:The purpose of this study was to examine the effect of perfusion of the colon with a fatty acid (oleic acid) on peptone-stimulated gastric acid secretion and release of gastrin in conscious dogs. Gastric acid secretion was monitored by continuous intragastric titration. Perfusion of the colon with sodium oleate (24 mm...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01297136
更新日期：1991-07-01 00:00:00
abstract：:Alterations of small intestinal sensory thresholds and small intestinal dysmotility are associated with functional dyspepsia. Because gastric and duodenal afferents partly project to the same areas, we postulated that patients with functional dyspepsia and H. pylori infection would be characterized by lower duodenal s...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02088548
更新日期：1996-07-01 00:00:00
abstract：:Alkaline sphingomyelinase (Alk-SMase) is a key enzyme in the intestinal tract for digestion of dietary sphingomyelin (SM), which generates lipid messengers with cell-cycle regulating effects. The enzyme is significantly decreased in ulcerative colitis and colon cancer. Based on this information, we wanted to investiga...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-008-0509-2
更新日期：2009-07-01 00:00:00
abstract：:MR enteroclysis is becoming a very important imaging modality in the workup and follow-up of small bowel diseases. The technique has many advantages, including the absence of ionizing radiation, ability to control luminal distension, superior tissue characterization, multiplanar capabilities, and mural and extramural ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-005-2700-z
更新日期：2005-06-01 00:00:00
abstract：:Cyclic GMP plays an important role in maintaining homeostasis in the gastric mucosa. NSAIDs damage the mucosa by mechanisms that may be mediated by alterations in the intragastric concentration of cyclic GMP. To test this hypothesis we studied the effects of the oral administration of acetylsalicylic acid (100, 300, a...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1023020201005
更新日期：2003-05-01 00:00:00
abstract：:The efficacy of comaruman CP, a pectin of marsh cinquefoil Comarum palustre L., was investigated using a model of acetic acid-induced colitis in mice. Mice were administered comaruman CP orally 2 days prior to rectal injection of 5% acetic acid and examined for colonic damage 24 hr later. Colonic inflammation was char...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-005-9034-8
更新日期：2006-09-01 00:00:00
abstract：:Lichen planus is a common mucocutaneus disorder that rarely involves the esophagus. We report two challenging cases presenting with recurrent dysphagia originally suspected due to gastroesophageal reflux. Subsequent evaluation revealed peculiar endoscopic findings of desquamative esophagitis leading to the diagnosis o...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章,评审
doi：10.1023/a:1011943805711
更新日期：2001-10-01 00:00:00
abstract：:The in vitro antioxidant capacity of sulfasalazine (SASP), its metabolites (SP, 5-ASA), and olsalazine (OAZ), was studied by evaluating their effects on superoxide (O2-.) production. Assay systems were the xanthine-xanthine oxidase (X/XOD) reaction and phorbol myristate acetate (PMA)-activated polymorphonuclear leukoc...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01300752
更新日期：1991-02-01 00:00:00
abstract：:With the growing popularity of propofol mediated deep sedation for upper gastrointestinal (GI) endoscopic procedures, challenges are being felt and appreciated. Research suggests that management of the airway is anything but routine in this setting. Although many studies and meta-analyses have demonstrated the safety ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章,评审
doi：10.1007/s10620-016-4375-z
更新日期：2017-01-01 00:00:00
abstract：:Upper gastrointestinal endoscopy was performed for the evaluation of infectious esophagitis in 19 consecutive subjects evaluated prospectively before orthotopic liver transplantation (OLTx), in a separate group of 27 subjects post-OLTx, and in 21 subjects following orthotopic renal transplantation (ORTx). None of the ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01535788
更新日期：1988-09-01 00:00:00
abstract：BACKGROUND AND AIMS:A prolific trend currently designates endoscopic ultrasonography (EUS) literature. We aimed to record all EUS-studies published during the past decade and evaluate them in terms of scientific quality, creating a stratification based on levels of evidence (LE). METHODS:All articles on EUS published ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章,评审
doi：10.1007/s10620-011-1961-y
更新日期：2012-03-01 00:00:00
abstract：BACKGROUND:Average-risk women aged 50-59 years have a lower incidence and mortality of colorectal cancer relative to age-matched men, calling into question the benefit of screening colonoscopy in this age group. AIMS:We aimed to determine whether FOBT is an effective initial screening test in 50-59-year-old women. ME...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-018-5156-7
更新日期：2018-10-01 00:00:00
