当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Detection of cannabinoid receptors by photoaffinity labelling.

Detection of cannabinoid receptors by photoaffinity labelling.

Abstract：

:A novel [125I]-labelled photoaffinity ligand designed to detect cannabinoid binding sites has been used in mouse brain preparations and in cultured S49 mouse lymphoma cells. The ligand, 2-iodo-5'-azido-delta 8-THC, shows a high affinity for sites in both brain (Kd = 5.60 pM) and whole cell (Kd = 9.38 pM) systems. Photolabelling studies with brain samples revealed the existence of four ligand-protein adducts, of estimated molecular weights 85.5, 62.1, 30.0 and 25.5 kDa, that were diminished by prior exposure to 8 microM THC. A similar study with S49 cells gave adducts with apparent molecular weights of 62.1, 34.4, 16.9 and 13.5 kDa. The ligand produces a typical cannabinoid cataleptic response in mice suggesting that possibly one or more of the binding sites may be involved in some of the receptor mediated actions of THC.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Burstein SH,Audette CA,Charalambous A,Doyle SA,Guo Y,Hunter SA,Makriyannis A

doi

10.1016/0006-291x(91)90951-3

subject

Has Abstract

pub_date

1991-04-15 00:00:00

pages

492-7

issue

1

eissn

0006-291X

issn

1090-2104

pii

0006-291X(91)90951-3

journal_volume

176

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • 1-bromoalkanes as new potent nontoxic glutathione depletors in isolated rat hepatocytes.

    abstract：:The effect of 1-bromlalkanes on intracellular glutathione (GSH) was studied in freshly isolated rat hepatocytes. Treatment of cells with bromoalkanes depleted cellular GSH levels without causing cytotoxicity. The extent of GSH depletion was directly proportional to the concentration and increasing chain length of 1-br...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91389-t

    authors： Khan S,O'Brien PJ

    更新日期：1991-08-30 00:00:00

  • PPARalpha activation by Wy 14643 induces transactivation of the rat UCP-1 promoter without increasing UCP-1 mRNA levels and attenuates PPARgamma-mediated increases in UCP-1 mRNA levels induced by rosiglitazone in fetal rat brown adipocytes.

    abstract：:Rodent brown adipocytes express peroxisome proliferator activated receptor-alpha (PPARalpha) and PPARgamma and while the rodent uncoupling protein-1 (UCP-1) gene contains a putative peroxisome proliferator response element (PPRE), only PPARgamma activation by thiazolidinediones increase UCP-1 mRNA levels. We have inve...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1526

    authors： Teruel T,Clapham JC,Smith SA

    更新日期：1999-10-22 00:00:00

  • S-Nitrosothiols increases cystic fibrosis transmembrane regulator expression and maturation in the cell surface.

    abstract：:S-nitrosothiols (SNOs) are endogenous signaling molecules with a broad spectrum of beneficial airway effects. SNOs are normally present in the airway, but levels tend to be low in cystic fibrosis (CF) patients. We and others have demonstrated that S-nitrosoglutathione (GSNO) increases the expression, maturation, and f...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.12.130

    authors： Zaman K,Bennett D,Fraser-Butler M,Greenberg Z,Getsy P,Sattar A,Smith L,Corey D,Sun F,Hunt J,Lewis SJ,Gaston B

    更新日期：2014-01-24 00:00:00

  • LY6/PLAUR domain containing 3 has a role in the maintenance of colorectal cancer stem-like cells.

    abstract：:Colorectal carcinoma (CRC) is one of the most frequently diagnosed cancers and the leading cause of cancer-related death for both men and women. Recent studies have revealed that a small sub-population of cancer cells, termed cancer stem-like cells (CSCs)/cancer-initiating cells (CICs), are endowed with tumor-initiati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.02.112

    authors： Wang L,Hirohashi Y,Ogawa T,Shen M,Takeda R,Murai A,Yamamoto E,Kubo T,Nakatsugawa M,Kanaseki T,Tsukahara T,Nishidate T,Okita K,Kutomi G,Sato N,Takemasa I,Torigoe T

    更新日期：2017-04-29 00:00:00

  • Complex role of protein kinase C in mediating the supramaximal inhibition of pancreatic secretion observed with cholecystokinin.

    abstract：:Protein kinase C appears to play an important, yet complex role in the supramaximal inhibition of pancreatic acinar cell secretion observed in response to cholecystokinin (CCK). The addition of protein kinase C activation to the concentration-response curve of a partial agonist acting at the CCK receptor (a phenethyl ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81522-0

    authors： Gaisano HY,Miller LJ

    更新日期：1992-08-31 00:00:00

  • Bacteriostatic properties of biomatrices against common orthopaedic pathogens.

    abstract：:Tissue-engineered grafts for tissue regeneration include either mature or progenitor cells seeded onto biomatrices that provide shape and support for developing tissue. Popular biomaterials used in orthopaedic surgery include collagen type I, hyaluronic acid, hydroxyapatite, and polylactic polyglycolic acid (PLGA). Bi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.06.165

    authors： Carlson GA,Dragoo JL,Samimi B,Bruckner DA,Bernard GW,Hedrick M,Benhaim P

    更新日期：2004-08-20 00:00:00

  • Phosphorylation of calcineurin: effect on calmodulin binding.

    abstract：:The effect of phosphorylation of calcineurin on calmodulin (CaM) binding was examined using a synthetic peptide which contains the CaM-binding domain and the serine phosphorylation site. The peptide, corresponding to residues 391-414 of brain calcineurin A subunit, was rapidly phosphorylated by protein kinase C and Ca...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90708-u

    authors： Calalb MB,Kincaid RL,Soderling TR

    更新日期：1990-10-30 00:00:00

  • Prosomatostatin processing in anglerfish brain, gut and pancreas.

    abstract：:The distribution of somatostatin immunoreactive forms in three tissues of the anglerfish (Lophius piscatorius L.) was analyzed by a combination of gel permeation, High Pressure Liquid Chromatography and amino acid analysis. The data indicate that prosomatostatins I and II are expressed in both neural and gastro-intest...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90600-6

    authors： Morel A,Kuks PF,Bourdais J,Cohen P

    更新日期：1988-02-29 00:00:00

  • Preparation of monomeric cytochrome C oxidase: its kinetics differ from those of the dimeric enzyme.

    abstract：:Bovine cytochrome c oxidase in 0.1% dodecylmaltoside, 50 mM KCl and 10 mM Tris-HCl, pH 7.4 is monodisperse with an apparent Mr 360,000 (dimer) as estimated by filtration on Ultrogel AcA 34. In the absence of added KCl the apparent Mr is 160,000 (monomer). The dimeric enzyme has a high and a low affinity site for cytoc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)90855-0

    authors： Nałecz KA,Bolli R,Azzi A

    更新日期：1983-07-29 00:00:00

  • Overexpression of adenine nucleotide translocase reduces Ca2+ signal transmission between the ER and mitochondria.

    abstract：:The adenine nucleotide translocase (ANT), besides transferring ATP from the mitochondrial matrix to the rest of the cell, has also been proposed to be involved in mitochondrial permeability transition (MPT), and accordingly in mitochondrial Ca2+ homeostasis. In order to assess the role of ANT in Ca2+ signal transmissi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.07.072

    authors： Wieckowski MR,Szabadkai G,Wasilewski M,Pinton P,Duszyński J,Rizzuto R

    更新日期：2006-09-22 00:00:00

  • A GSK3beta phosphorylation site in axin modulates interaction with beta-catenin and Tcf-mediated gene expression.

    abstract：:Upon binding of a Wnt to its receptor, GSK3beta is inhibited through an unknown mechanism involving Dishevelled (Dsh), resulting in the dephosphorylation and stabilization of beta-catenin, which translocates to the nucleus and interacts with Lef/Tcf transcription factors to activate target gene expression. Axin is a s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1760

    authors： Jho Eh,Lomvardas S,Costantini F

    更新日期：1999-12-09 00:00:00

  • Pharmacological inhibition of Rac1 exerts a protective role in ischemia/reperfusion-induced renal fibrosis.

    abstract：:Acute kidney injury induced by renal ischemia-reperfusion (IR) is a prominent risk factor in the development towards renal fibrosis. Ras-related C3 botulinum toxin substrate 1(Rac1) has been involved in the pathophysiology of fibrotic disorders. But the role of Rac1 in the pathogenesis of IR-induced renal fibrosis is ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.07.009

    authors： Liang H,Huang J,Huang Q,Xie YC,Liu HZ,Wang HB

    更新日期：2018-09-18 00:00:00

  • Role of ErbB2 in the prostaglandin E₂-induced enhancement of the mitogenic response to epidermal growth factor in cultured hepatocytes.

    abstract：:Prostaglandin E(2) (PGE(2)) enhances the mitogenic response to epidermal growth factor (EGF) in hepatocytes, but the underlying mechanisms are not clear. We previously observed that PGE(2) upregulates EGF-induced signalling in the MEK/ERK and PI3K/Akt pathways in hepatocytes. Other investigations have indicated that E...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.03.148

    authors： Ødegård J,Aasrum M,Tveteraas IH,Bharath SP,Sandnes D,Christoffersen T

    更新日期：2012-05-04 00:00:00

  • On the mechanism of cytosolic phospholipase A2 activation in CHO cells carrying somatostatin receptor: wortmannin-sensitive pathway to activate mitogen-activated protein kinase.

    abstract：:We examined the mechanism of arachidonate release induced by somatostatin-14 (SS14) in CHO-K1 cells overexpressing rat hippocampal somatostatin receptor SSTR4. SSTR4 couples to pertussis toxin (PTX)-sensitive G-protein in CHO cells and does not lead to phosphoinositides breakdown or intracellular calcium ([Ca2+]i) mob...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2623

    authors： Sakanaka C,Ferby I,Waga I,Bito H,Shimizu T

    更新日期：1994-11-30 00:00:00

  • Engineered peptides corresponding to segments of the H3 domain of syntaxin inhibit insulin release both in intact and permeabilized mouse pancreatic beta cells.

    abstract：:Syntaxin is one of the proteins involved in the exocytotic event through sequential binding to specific proteins, including SNAP25 and synaptobrevin. In a previous work in digitonin-permeabilized beta cells, we characterized the functional role of two segments: synA and synB of the H3 domain of syntaxin. As a continua...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8923

    authors： Martin F,Salinas E,Barahona F,Vázquez J,Soria B,Reig JA

    更新日期：1998-07-09 00:00:00

  • The modulation of growth by HMBA in PKC overproducing HT29 colon cancer cells.

    abstract：:To examine whether protein kinase C (PKC) plays a role in mediating growth inhibitory effects of hexamethylene bisacetamide (HMBA) we compared a control H29 colon cancer cell line to a derivative, HT29-PKC7, that overexpresses high levels of PKC beta 1. We found that although HMBA markedly inhibited the growth of the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91262-b

    authors： Choi PM,Weinstein IB

    更新日期：1991-12-16 00:00:00

  • Torsion of the central pair microtubules in eukaryotic flagella due to bending-driven lateral buckling.

    abstract：:Inspired by recent interest in torsion of the central pair microtubules in eukaryotic flagella, a novel thin-walled elastic beam model is suggested to study critical condition under which uniform bending of a flagellum will cause lateral/torsional buckling of the central pair. The model is directed to the central pair...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.10.019

    authors： Li C,Ru CQ,Mioduchowski A

    更新日期：2006-12-08 00:00:00

  • The class I histone deacetylases of the platyhelminth parasite Schistosoma mansoni.

    abstract：:Histone deacetylases (HDAC) form a conserved enzyme family that control gene expression via the removal of acetyl residues from histones and other proteins and are under increasing investigation as therapeutic targets, notably in cancer and parasitic diseases. To investigate the conservation of these enzymes in the pl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.10.090

    authors： Oger F,Dubois F,Caby S,Noël C,Cornette J,Bertin B,Capron M,Pierce RJ

    更新日期：2008-12-26 00:00:00

  • The effects of differential gene expression on coding sequence features: analysis by one-way ANOVA.

    abstract：:It is well-established that non-random patterns in coding DNA sequence (CDS) features can be partially explained by translational selection. Recent extensions of microarray and proteomic expression data have stimulated many genome-wide investigations of the relationships between gene expression and various CDS feature...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.05.043

    authors： Wu G,Nie L,Freeland SJ

    更新日期：2007-07-13 00:00:00

  • Stretching mesangial cells stimulates tyrosine phosphorylation of focal adhesion kinase pp125FAK.

    abstract：:Mechanical loading of cultured mesangial cells has been shown to induce both increased production of matrix proteins and cell proliferation. This in vitro phenomenon has been implicated in the progression of glomerular sclerosis observed in a variety of glomerular diseases. However, it is not yet known how cells sense...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2004

    authors： Hamasaki K,Mimura T,Furuya H,Morino N,Yamazaki T,Komuro I,Yazaki Y,Nojima Y

    更新日期：1995-07-17 00:00:00

  • Tumorigenesis of a v-Ha-ras-expressing pre-B cell line selects for c-myc activation.

    abstract：:Seven tumors independently derived from a v-Ha-ras-expressing pre-B cell line were examined to determine the oncogene activations cooperating with v-Ha-ras in in vivo tumor progression. The pre-B cell line was generated by infection with Moloney murine leukemia virus (MoMuLV) and a MoMuLV-derived recombinant expressin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91041-a

    authors： Chen SC,Redenius D,Schwartz RC

    更新日期：1991-08-15 00:00:00

  • UV light induces premature senescence in Akt1-null mouse embryonic fibroblasts by increasing intracellular levels of ROS.

    abstract：:Akt/PKB plays a pivotal role in cell survival and proliferation. Previously, we reported that UV-irradiation induces extensive cell death in Akt2(-/-) mouse embryonic fibroblasts (MEFs) while Akt1(-/-) MEFs show cell cycle arrest. Here, we find that Akt1(-/-) MEFs exhibit phenotypic changes characteristics of senescen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.04.017

    authors： Jee HJ,Kim HJ,Kim AJ,Bae YS,Bae SS,Yun J

    更新日期：2009-06-05 00:00:00

  • Crystal structure of human mitochondrial acyl-CoA thioesterase (ACOT2).

    abstract：:Acyl-CoA thioesterases (ACOTs) catalyze the hydrolysis of CoA esters to free CoA and carboxylic acids and have important functions in lipid metabolism and other cellular processes. Type I ACOTs are found only in animals and contain an alpha/beta hydrolase domain, through currently no structural information is availabl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.05.122

    authors： Mandel CR,Tweel B,Tong L

    更新日期：2009-08-07 00:00:00

  • CTCF deficiency causes expansion of the sensory domain in the mouse cochlea.

    abstract：:The cochlea in the mammalian inner ear is a sensitive and sharply organized sound-detecting structure. The proper specification of neurosensory-competent domain in the otic epithelium is required for the formation of mature neuronal and sensory domains. Genetic studies have provided many insights into inner ear develo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.03.127

    authors： Ma JH,Kim HP,Shin JO

    更新日期：2019-05-14 00:00:00

  • Tumor necrosis factor-alpha increased the integrin alpha 2 beta 1 expression and cell attachment to type I collagen in human dermal fibroblasts.

    abstract：:Cell adhesion molecules of human dermal fibroblasts play an important role in the processes of wound healing. The effects of tumor necrosis factor- alpha (TNF) on the expression of integrin beta 1 subfamily in human dermal fibroblasts were examined. TNF preferentially induced the expression of alpha 2 beta 1 integrins...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1411

    authors： Ezoe K,Horikoshi T

    更新日期：1993-04-15 00:00:00

  • Protein kinase C binding to isolated nuclei and its activation by a Ca2+/phospholipid-independent mechanism.

    abstract：:The direct interaction of protein kinase C with the nucleus was examined utilizing endogenous protein phosphorylation and [3H]PDBu binding to detect the enzyme. Rat brain nuclei were relatively rich in phorbol ester receptors whereas liver nuclei contained less than 10% of their brain counterpart. Purified protein kin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)91030-8

    authors： Misra UK,Sahyoun N

    更新日期：1987-06-15 00:00:00

  • Treatment with insulin-like growth factor 1 receptor inhibitor reverses hypoxia-induced epithelial-mesenchymal transition in non-small cell lung cancer.

    abstract：:Insulin-like growth factor 1 receptor (IGF1R) is expressed in many types of solid tumors including non-small cell lung cancer (NSCLC), and enhanced activation of IGF1R is thought to reflect cancer progression. Epithelial-mesenchymal transition (EMT) has been established as one of the mechanisms responsible for cancer ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.11.014

    authors： Nurwidya F,Takahashi F,Kobayashi I,Murakami A,Kato M,Minakata K,Nara T,Hashimoto M,Yagishita S,Baskoro H,Hidayat M,Shimada N,Takahashi K

    更新日期：2014-12-12 00:00:00

  • Specific uptake of succinylated proteins via a scavenger receptor-mediated mechanism in cultured brain microvessel endothelial cells.

    abstract：:Cellular uptake of succinylated catalase (Suc-CAT; Mw 227 kDa), bovine serum albumin (Suc-BSA; Mw 70 kDa), superoxide dismutase (Suc-SOD; Mw 34 kDa) and soybean trypsin inhibitor (Suc-STI; Mw 21 kDa) was studied using primary cultures of bovine brain microvessel endothelial cells (BMECs) developed as an in vitro model...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2210

    authors： Tokuda H,Masuda S,Takakura Y,Sezaki H,Hashida M

    更新日期：1993-10-15 00:00:00

  • Regulated expression of 5'-deleted mouse GLUT4 minigenes in transgenic mice: effects of exercise training and high-fat diet.

    abstract：:Fourteen kb murine GLUT4 minigene (= -7395 GLUT4) contains DNA sequence that confers tissue specific, exercise-induced up-regulation of the GLUT4 gene in skeletal muscle and high-fat diet induced-down-regulation in white adipose tissue. To identify the DNA sequences required for regulated expression, we generated GLUT...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7516

    authors： Tsunoda N,Cooke DW,Ikemoto S,Maruyama K,Takahashi M,Lane MD,Ezaki O

    更新日期：1997-10-20 00:00:00

  • Low-level oxidative stress causes cell-cycle specific arrest in cultured cells.

    abstract：:Oxidative stress is involved in a multitude of pathological conditions. In the present study, cell cycle arrest was demonstrated in monolayer cultures of Chinese hamster ovary cells subjected to low-level oxidative stress induced by the addition of hydrogen peroxide. Fluorescence-activated cell sorting analysis charac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1645

    authors： Clopton DA,Saltman P

    更新日期：1995-05-05 00:00:00