Abstract:
:Laser scanning cytometry (LSC) is a powerful tool for qualitative and quantitative analysis of tissue sections in preclinical drug development. LSC combines the strengths of flow cytometry with tissue architecture retention. This technology has been used predominantly with immunofluorescent techniques on cell culture and tissue sections, but recently LSC has shown promise in evaluating chromogenic immunohistochemistry (IHC) and histochemical products in paraffin-embedded and/or frozen tissue sections. Inverted light scatter measurements or a combination of inverted scatter and fluorescence allows automated determination of cell/nuclear counts (e.g., proliferation labeling indices), cell area (e.g., cellular hypertrophy), stromal elements, and labeling intensity (e.g., cytoplasmic/organellar proteins) in chromogen-labeled IHC or histochemical stained sections that correlates well with standard manual quantification methods. Segmentation with autofluorescence or dual immunolabeling facilitates capture of labeling data from specific cell populations. LSC evaluation of HE-stained sections is accomplished using autofluorescence/eosin fluorescence and inverse scatter. A standardized fluorescent approach with archivability, a lack of fluorescence quenching (photobleaching), and amenability to evaluation of multiple markers in a section has been demonstrated using Qdot nanocrystals. Examples of LSC use in chromogenic IHC, routine histopathology, and Qdot labeling will be reviewed, and advantages and disadvantages of this technology will be discussed.
journal_name
Toxicol Patholjournal_title
Toxicologic pathologyauthors
Peterson RA,Krull DL,Butler Ldoi
10.1177/0192623307312704subject
Has Abstractpub_date
2008-01-01 00:00:00pages
117-32issue
1eissn
0192-6233issn
1533-1601pii
36/1/117journal_volume
36pub_type
杂志文章,评审abstract::Contrast-induced nephropathy (CIN) refers to a decline in renal function following exposure to iodinated contrast media (CM). The present study was initiated to explore the role of known human risk factors (spontaneous hypertension, diabetes, protein-losing nephropathy) on CIN development in rodent models and to deter...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623312464122
更新日期:2013-01-01 00:00:00
abstract::Blood platelets play a fundamental role in hemostasis and thrombosis. The physiological role of platelets is to preserve the integrity of the cardiovascular system such that upon injury platelets are activated and serve to interrupt a potentially deleterious situation (i.e., hemorrhage). However, platelets are also in...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339302100210
更新日期:1993-01-01 00:00:00
abstract::Existing liver perfusion models are largely limited by high degrees of ischemic and reperfusion injury and the lack of standardization. To establish a highly standardized perfusion model and minimize reperfusion injury, a porcine liver perfusion model was developed using an artificial heart pump (Buecherl Artificial H...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1080/01926230590958164
更新日期:2005-01-01 00:00:00
abstract::Sensitive kidney safety assessment is important for successful drug development in both preclinical and clinical stages. The Food and Drug Administration recently qualified a composite measure of 6 urine creatinine-normalized biomarkers, such as clusterin, cystatin C, kidney injury molecule 1 (KIM-1), N-acetyl-β-d-glu...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623320964391
更新日期:2020-12-01 00:00:00
abstract::The usefulness of isolated adult cardiac myocytes, both in suspension and in culture, as a model system for measuring cardiotoxicity is evaluated. It has been suggested that isolated adult myocytes should preferably be cultured for such experimentation, as this restores the cells to a physiological preference for fatt...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::A variety of phenotypic cellular changes emerge in the liver of different species prior to the appearance of hepatocellular adenomas and carcinomas induced by carcinogenic agents (chemicals, radiation, hepadna viruses) or develop "spontaneously." Foci of altered hepatocytes have been studied most extensively in rats t...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1177/0192623389017004107
更新日期:1989-01-01 00:00:00
abstract::Clinical, pathologic, and analytical records from 200 cattle were reviewed to determine if long-term exposures to elevated fluorides resulted in previously unrecognized or unreported pathologic changes, especially skeletal neoplasia. Animals were part of comprehensive field and laboratory investigations of bovine fluo...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339202000216
更新日期:1992-01-01 00:00:00
abstract::During the conduct of in vivo toxicology studies, in-life, clinical pathology, and anatomic pathology parameters are collected and interpreted. These sets of parameters are evaluated in an integrative manner to determine the overall toxicity of a test article. For clinical pathology parameters, the inherent variabilit...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623314555340
更新日期:2015-01-01 00:00:00
abstract::Parenteral administration of recombinant hematopoietic growth factors has been sporadically associated with cutaneous complications, including injection site reactions in humans and nonhuman primates. In this study, subcutaneous injection sites were evaluated from 12 cynomolgus monkeys administered a recombinant human...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339602400401
更新日期:1996-07-01 00:00:00
abstract::The uterine cellular expression and distribution of c-fos, ERalpha and p38alpha was compared in humans, nonhuman primates, and rats using immunohistochemistry. ERalpha and c-fos were present in the glandular (GE) and luminal epithelial cells (LE) of humans and nonhuman primates, with differing expression patterns evid...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1080/01926230600773941
更新日期:2006-01-01 00:00:00
abstract::With the increasing use of animal-based biomaterials for regenerative medical applications, the need for their safety assessment is paramount. A porcine cholecyst-derived scaffold (CDS), intended as a muscle repair graft, prepared by a nondetergent/enzymatic method was engrafted in a rat abdominal wall defect model. H...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623317752894
更新日期:2018-02-01 00:00:00
abstract::Toxicogenomics using a reference database can provide a better understanding and prediction of toxicity, largely by creating biomarkers that tie gene expression to actual pathology events. During the course of building a toxicogenomic database, an observation was made that a number of non-steroidal anti-inflammatory c...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1080/01926230600611752
更新日期:2006-01-01 00:00:00
abstract::Our previous studies showed that protein kinase Cepsilon (PKCepsilon) verexpression in mouse skin resulted in metastatic squamous cell carcinoma (SCC) elicited by single 7,12-dimethylbenz(a)anthracene (DMBA)-initiation and 12-O-tetradecanoylphorbol-13-acetate (TPA)-promotion in the absence of preceding papilloma forma...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1080/01926230701748164
更新日期:2007-12-01 00:00:00
abstract::The dose dependence of L-ascorbic acid (AsA) copromotion of urinary bladder carcinogenesis with continuous concomitant administration of NaHCO3 was investigated. In the first experiment, 83 male F344 rats were all given 0.05% N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) for 4 wk and then divided into 5 groups, which re...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339702500306
更新日期:1997-05-01 00:00:00
abstract::GJA1 gene (Connexin43, also known as Cx43) is the most abundant gap junction protein isoform in animal cells and is associated with bone development in embryos. The objective of the present work was to evaluate in vivo osteal development in GJA1-deficient fetal mice through determination of the histological and molecu...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623311422075
更新日期:2011-12-01 00:00:00
abstract::The handling-induced dark neuron is a histological artifact observed in brain samples handled before fixation with aldehydes. To explore associations between dark neurons and immunohistochemical alterations in mouse brains, we examined protein products encoded by Cav3 (neuronal perikarya/neurites), Rbbp4 (neuronal nuc...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623319896263
更新日期:2020-04-01 00:00:00
abstract::A retrospective analysis of microscopic evaluation data from control (device and/or saline-treated) animals in intrathecal studies in monkeys, dogs, sheep, and rats was conducted. The studies were performed by multiple testing facilities. All slide preparation and microscopic evaluation were conducted in the laborator...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623310391679
更新日期:2011-01-01 00:00:00
abstract::The expression of specific cytokeratin (CK) polypeptide patterns is a sensitive marker of the cytoskeletal differentiation of epithelial cells. We developed an immunohistochemical method to assess CK expression patterns in the rat respiratory tract using serial paraffin-embedded sections from the nasal cavity, trachea...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339802600308
更新日期:1998-05-01 00:00:00
abstract::Histopathologic examination of the testis from juvenile rats is often necessary to characterize the safety of new drugs for pediatric use and is a required end point in male pubertal development and thyroid function assays. To aid in evaluation and interpretation of the immature testis, the characteristic histologic f...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623314547279
更新日期:2015-04-01 00:00:00
abstract::The role of articular chondrocytes and matrix degrading enzymes such as collagenase and neutral protease in the pathogenesis of quinolone-induced cartilage degeneration was investigated in immature guinea pigs. Articular cartilage from nalidixic acid (NA) treated guinea pigs was examined for the presence of protease a...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339001800209
更新日期:1990-01-01 00:00:00
abstract::The B6C3F1 strain of mice is prone to develop liver nodules as animals grow older. This spontaneous tumor development is enhanced by dietary lipotrope deficiency. The present studies were performed to evaluate the liver of the B6C3F1 mice in early periods of lipotrope deficiency and before the nodules appear. Mice wer...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262338501300103
更新日期:1985-01-01 00:00:00
abstract::Cytoplasmic vacuolation of sensory neurons has been reported to occur within the dorsal root ganglia in studies investigating various neuropathic conditions including the effects of neurotoxic chemicals. In this study, we investigated this lesion in adult (98-119 days old) male Long-Evans rats, after multiple exposure...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623310369343
更新日期:2010-06-01 00:00:00
abstract::An eosinophilic substance (ES) is usually observed in the mouse nasal septum. In contrast to textbooks and one report describing ES as amyloid, a previous study by the authors revealed that ES is not amyloid but consists of collagen and an amorphous material. Furthermore, it was suggested that the amorphous material w...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623310367805
更新日期:2010-06-01 00:00:00
abstract::7,12-Dimethylbenz[a]anthracene (DMBA) presents a pollutant implicated in various toxicological effects. The aim of this experiment was to study the effects of DMBA administration on oxidative stress, histopathological signs, and 18 kDa translocator protein (TSPO) binding characteristics in rat liver. We also studied t...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623310379137
更新日期:2010-10-01 00:00:00
abstract::The title of the 2017 Society of Toxicologic Pathology symposium was "Musculoskeletal System." A brief overview of the General Scientific Symposium is presented herein and describes the topics presented by each speaker. Symposium speakers addressed subjects pertinent to musculoskeletal system toxicologic pathology and...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1177/0192623317738211
更新日期:2017-10-01 00:00:00
abstract::I was honored to be the keynote speaker at the 30th Annual Society of Toxicologic Pathology Symposium "Toxicologic Pathology and the Immune System." I had the opportunity to reminisce about events in the 1970s that set the stage for the birth and subsequent growth of the field of immunotoxicology and to summarize my r...
journal_title:Toxicologic pathology
pub_type: 历史文章
doi:10.1177/0192623311427710
更新日期:2012-01-01 00:00:00
abstract::Specific regions in the rodent larynx exhibit cellular changes in response to inhaled xenobiotics. These regions include the base of the epiglottis, ventral pouch, and medial surfaces of the vocal processes of the arytenoid cartilages. There are interspecies differences among laboratory rodents in the microscopic anat...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339302100604
更新日期:1993-11-01 00:00:00
abstract::The objectives of this session were to explore causes of variability in clinical pathology data due to preanalytical and analytical variables as well as study design and other procedures that occur in toxicity testing studies. The presenters highlighted challenges associated with such variability in differentiating te...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623316675766
更新日期:2017-01-01 00:00:00
abstract::Bioabsorbable implants can be advantageous for certain surgical tissue bioengineering applications and implant-assisted tissue repair. They offer the obvious benefits of nonpermanence and eventual restoration of the native tissue's biomechanical and immunological properties, while providing a structural scaffold for h...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1177/0192623318816681
更新日期:2019-04-01 00:00:00
abstract::The clinical, laboratory, and pathologic features of a syndrome in dogs characterized by intermittent pain, fever, neutrophilia, and necrotizing arteritis are described to alert others involved in toxicity testing to the existence of this disorder. It is considered that this idiopathic syndrome is a latent condition, ...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262338901700109
更新日期:1989-01-01 00:00:00