Abstract：
:The usefulness of isolated adult cardiac myocytes, both in suspension and in culture, as a model system for measuring cardiotoxicity is evaluated. It has been suggested that isolated adult myocytes should preferably be cultured for such experimentation, as this restores the cells to a physiological preference for fatty acids as a substrate rather than glucose. We show here that the restoration of Ca2+ to cells immediately after isolation results in an artificially enhanced glucose metabolism, as measured by an elevated rate of deoxyglucose uptake. Early restoration of Ca2+ during cell isolation, on the other hand, results in cells with a normal low level of deoxyglucose uptake. We, thus, conclude that cells can be ready for valid toxicity studies immediately after isolation, without the need for culture. The culture of adult feline ventricular cells is also described. These cells, like rabbit but unlike rat, are particularly promising for toxicity studies because they remain quiescent in culture and do not round up. On exposure to norepinephrine, they beat spontaneously and increase their rate of protein synthesis.
journal_name
Toxicol Patholjournal_title
Toxicologic pathologyauthors
Haworth RA,Goknur AB,Cook MG,Decker RSsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
511-20issue
4 Pt 1eissn
0192-6233issn
1533-1601journal_volume
18pub_type
杂志文章abstract：:Studies were carried out on microsomes isolated from the highly differentiated (slow-growing) Morris hepatoma 9618A, on microsomes and plasma membranes from the poorly differentiated (fast-growing) Morris hepatoma 3924A, and rat liver used as control. The lipid composition (phospholipid and cholesterol content, degree...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/019262338401200404
更新日期：1984-01-01 00:00:00
abstract：:The cochlea is one of the more common targets for toxic effects, yet current toxicologic screening in animals does not routinely evaluate the cochlea as a potential target organ. Although histopathologic sections are routinely taken from the eye and the optic nerve and tract and most studies include at least 1 section...
journal_title：Toxicologic pathology
pub_type： 杂志文章,评审
doi：10.1177/019262330002800117
更新日期：2000-01-01 00:00:00
abstract：:Toxicology of the male reproductive system has received increased interest in recent years partly fuelled by the growing reports of falling sperm counts and rising reproductive disorders in the human population. Recently revised regulatory guidelines for the safety assessment of pharmaceuticals and chemicals on reprod...
journal_title：Toxicologic pathology
pub_type： 杂志文章,评审
doi：10.1177/019262339702500201
更新日期：1997-03-01 00:00:00
abstract：:In vitro techniques for the culture of hemopoietic stem cells and committed hemopoietic progenitor cells in rat bone marrow have not been adequately described in the literature. In the present investigations, and using commercially available hemopoietic cytokines and growth factors, the conditions required to perform ...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/0192623308328133
更新日期：2009-02-01 00:00:00
abstract：:Results of early nonclinical "General Toxicology" studies are used to set a safe starting dose for first-in-human (FIH) clinical trials. In FIH trials, the research subjects are typically healthy volunteers who have little to gain but much to lose if a trial goes wrong. With that in mind, good laboratory practice regu...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/0192623313506878
更新日期：2014-01-01 00:00:00
abstract：:Investigations in toxicologic neuropathology are complex undertakings because of the intricate spatial and temporal diversity in the anatomic, functional, and molecular organization of the central and peripheral nervous systems. This compilation of toxicologic neuropathology resources has been designed to consolidate ...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/0192623310385142
更新日期：2011-01-01 00:00:00
abstract：:Toxic injury occurs in the liver more often than any other organ. This can be attributed to the fact that virtually all ingested substances that are absorbed are first presented to the liver and that the liver is responsible for the metabolism and elimination of many substances. Drug-induced liver injury has become a ...
journal_title：Toxicologic pathology
pub_type： 杂志文章,评审
doi：10.1080/01926230590522428
更新日期：2005-01-01 00:00:00
abstract：:This experiment evaluated effects following chronic toxicity with 20 ppm fenvalerate (synthetic pyrethroid), 2 ppm monocrotophos (organophosphate) and 2 ppm endosulfan (chlorinated hydrocarbon) on bone health of broiler chicks. A total of 120 chicks were divided equally into 4 groups and were fed poultry mash without ...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1080/01926230490431745
更新日期：2004-05-01 00:00:00
abstract：:Hepatic changes induced by phenethyl isothiocyanate (PEITC) in the liver of rats were determined by quantitative microscopy. Groups of male Fischer-344 rats were fed either a standard, cereal-based diet (Wayne rodent meal) or a purified diet (AIN-76A) containing PEITC at concentrations of 0.75 and 6.0 mmol/kg for 13 w...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/019262339502300602
更新日期：1995-11-01 00:00:00
abstract：:A semiautomated quantitative assay for rat serum alkaline phosphatase (ALP) isoenzyme determination was developed, incorporating selective precipitation of bone alkaline phosphatase (BALP) with wheat germ lectin and differential inhibition with levamisole for determination of intestinal alkaline phosphatase (IALP). Th...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/019262339402200607
更新日期：1994-11-01 00:00:00
abstract：:We examined the localization and expression of the nuclear oncoprotein c-myc and the cytoplasmic membrane-associated oncoprotein c-ras in rainbow trout cell lines derived from both normal and tumor tissue in order to question whether c-myc and ras oncoprotein immunostaining was increased in cells derived from tumors c...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/019262339602400310
更新日期：1996-05-01 00:00:00
abstract：:The carcinogenic potential of misoprostol, a synthetic prostaglandin E1 analogue with anti-ulcer potential, was evaluated in CD Sprague-Dawley rats. The compound was given daily by gavage at 24, 240, and 2,400 micrograms/kg, up to 150 times the daily human dose for 2 years. Necropsies were done on all animals and the ...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/019262338701500201
更新日期：1987-01-01 00:00:00
abstract：:Cyclodextrins are oligosaccharides which are used in the pharmaceutical industry and research as vehicles for application of apolar substances such as steroids. The aim of this study was to examine the long-term effects of parenteral administration of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) on bone. Sham-operated (SH...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/0192623312441405
更新日期：2012-07-01 00:00:00
abstract：:Reticulum cell hyperplasia (RCH) was a term used for many years by the National Toxicology Program (NTP) to describe a certain non-neoplastic bone marrow lesion of rats. Retrospective microscopic evaluation of RCH lesions and immunohistochemistry analyses were performed to reassess and further characterize these lesio...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/0192623319890610
更新日期：2020-02-01 00:00:00
abstract：:An annular, reddened lesion with mild serosal hemorrhage and no tumorous mass formation was detected in the right uterine horn of a 37-week-old female Sprague-Dawley rat that had postpubertal vaginal bleeding. Histological examination revealed prominent proliferation of the endometrium, which occupied the uterine lume...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/0192623313476575
更新日期：2013-01-01 00:00:00
abstract：:Groups of C57BL6 mice of each sex were assigned to one of 2 dietary regimens, ad libitum (AL) or dietary restriction (DR), to study effects of food restriction on body weight, survival, and neoplasia. The AL and DR groups were subdivided into a scheduled sacrifice group for examination at 6-mo intervals, and a lifetim...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/019262339502300503
更新日期：1995-09-01 00:00:00
abstract：:Standard histology trimming practices for toxicity studies in dogs include preparation of a section of the papillary muscle associated with the left ventricular free wall-the anteriolateral or anterior muscle. In contrast, the posteromedial or posterior papillary muscle, associated with the interventricular septum, is...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/0192623320963020
更新日期：2020-10-01 00:00:00
abstract：:The role of articular chondrocytes and matrix degrading enzymes such as collagenase and neutral protease in the pathogenesis of quinolone-induced cartilage degeneration was investigated in immature guinea pigs. Articular cartilage from nalidixic acid (NA) treated guinea pigs was examined for the presence of protease a...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/019262339001800209
更新日期：1990-01-01 00:00:00
abstract：:Histopathologic examination of the testis from juvenile rats is often necessary to characterize the safety of new drugs for pediatric use and is a required end point in male pubertal development and thyroid function assays. To aid in evaluation and interpretation of the immature testis, the characteristic histologic f...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/0192623314547279
更新日期：2015-04-01 00:00:00
abstract：:ISIS 2302 is a phosphorothioate oligodeoxynucleotide with a sequence complementary to the mRNA of human intercellular adhesion molecule 1 (ICAM-1). Hybridization of ISIS 2302 to the mRNA inhibits expression of the ICAM-1 protein in response to inflammatory stimuli. A murine active antisense oligonucleotide, ISIS 3082,...
journal_title：Toxicologic pathology
pub_type： 杂志文章,评审
doi：10.1177/019262339902700117
更新日期：1999-01-01 00:00:00
abstract：:A variety of phenotypic cellular changes emerge in the liver of different species prior to the appearance of hepatocellular adenomas and carcinomas induced by carcinogenic agents (chemicals, radiation, hepadna viruses) or develop "spontaneously." Foci of altered hepatocytes have been studied most extensively in rats t...
journal_title：Toxicologic pathology
pub_type： 杂志文章,评审
doi：10.1177/0192623389017004107
更新日期：1989-01-01 00:00:00
abstract：:The title of the 2017 Society of Toxicologic Pathology symposium was "Musculoskeletal System." A brief overview of the General Scientific Symposium is presented herein and describes the topics presented by each speaker. Symposium speakers addressed subjects pertinent to musculoskeletal system toxicologic pathology and...
journal_title：Toxicologic pathology
pub_type： 杂志文章,评审
doi：10.1177/0192623317738211
更新日期：2017-10-01 00:00:00
abstract：:The kerosene-type jet fuel, JP-8, consists of a complex mixture of aliphatic and aromatic hydrocarbons. Because of the utility of JP-8, studies have been conducted to identify the potential long-term consequence of occupational inhalation exposure. Fischer 344 rats and C57BL/6 mice of both sexes were exposed to JP-8 v...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/019262339101900201
更新日期：1991-01-01 00:00:00
abstract：:Pepsinogens are acid protease enzymes of pepsin usually found in gastric mucosa. In the present study, we demonstrated the presence of pepsinogen isozymes in male Syrian golden hamster lung tissues by a combined immunohistochemical and biochemical approach. Immunohistochemically, using rat pepsinogen 1 antibody, pepsi...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/019262339101900305
更新日期：1991-01-01 00:00:00
abstract：:The mechanisms underlying the development of gastrointestinal (GI) damage by the NSAIDs differ considerably from drug to drug. Aside from environmental or intersubject influence (e.g., concurrent disease, physical or sociopsychologic stress, dietary and genetic status), the intrinsic pharmacokinetic and physicochemica...
journal_title：Toxicologic pathology
pub_type： 杂志文章,评审
doi：10.1177/019262338801600218
更新日期：1988-01-01 00:00:00
abstract：:A number of factors related to study design have the potential to impact clinical pathology test results during the conduct of nonclinical safety studies. A thorough understanding of these factors is paramount in drawing accurate conclusions from clinical pathology data generated during such studies, particularly when...
journal_title：Toxicologic pathology
pub_type： 杂志文章,评审
doi：10.1177/0192623316677066
更新日期：2017-02-01 00:00:00
abstract：:The outcome of cardiac ischemic events depends not only on the extent and duration of the ischemic stimulus but also on the myocardial intrinsic tolerance to ischemic injury. Cardiac ischemic tolerance reflects myocardial functional reserves that are not always used when the tissue is appropriately oxygenated. Ischemi...
journal_title：Toxicologic pathology
pub_type： 杂志文章,评审
doi：10.1177/0192623309339503
更新日期：2009-08-01 00:00:00
abstract：:S-(1,2-dichlorovinyl)-L-cysteine (DCVC)-induced nephrotoxicity in vivo was investigated in New Zealand White rabbits. A primary emphasis in these studies was further characterization of DCVC-induced nephrotoxicity using a variety of serum and urinary analytes, including sodium dodecyl sulfate-polyacrylamide gel electr...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/019262339502300405
更新日期：1995-07-01 00:00:00
abstract：:This report documents the histological changes in nude mouse skin and in human skin xenografts on nude mice following exposure to phenyldichloroarsine (PDA), a vesicant arsenical. Under light microscopy, we observed in PDA-treated human skin grafts: 1) degeneration of epidermal cell nuclei (apparent by 2 hr after expo...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/019262338701500204
更新日期：1987-01-01 00:00:00
abstract：:With the increasing use of animal-based biomaterials for regenerative medical applications, the need for their safety assessment is paramount. A porcine cholecyst-derived scaffold (CDS), intended as a muscle repair graft, prepared by a nondetergent/enzymatic method was engrafted in a rat abdominal wall defect model. H...
journal_title：Toxicologic pathology
pub_type： 杂志文章
doi：10.1177/0192623317752894
更新日期：2018-02-01 00:00:00
