Abstract:
:The role of articular chondrocytes and matrix degrading enzymes such as collagenase and neutral protease in the pathogenesis of quinolone-induced cartilage degeneration was investigated in immature guinea pigs. Articular cartilage from nalidixic acid (NA) treated guinea pigs was examined for the presence of protease activity or the ex vivo synthesis of collagenase at various times post-treatment. Histologic evaluation of knee joints confirmed the presence of degenerative changes in the matrix, but increased collagenase synthesis or protease activity were not detected. A separate group of animals was used to determine the importance of articular chondrocytes in the lesion generation. These cells were killed by intra-articular injection of the glycolysis inhibitor iodoacetic acid (IA) prior to treatment of the animals with NA. Typical "blister-like" their presence was not required for lesion development. Cartilage exposed to IA only did not exhibit "blister-like" lesions indicating that chondrocyte death and proteoglycan loss in conjunction with frictional forces associated with load-bearing were not sufficient to induce major matrical disruptive changes during the period of this study. These results indicate that articular chondrocytes are not actively involved in inducing the degenerative changes and provide no evidence for involvement of collagenase or neutral protease in the pathogenesis of the lesion.
journal_name
Toxicol Patholjournal_title
Toxicologic pathologyauthors
Bendele AM,Hulman JF,Harvey AK,Hrubey PS,Chandrasekhar Sdoi
10.1177/019262339001800209subject
Has Abstractpub_date
1990-01-01 00:00:00pages
304-12issue
2eissn
0192-6233issn
1533-1601journal_volume
18pub_type
杂志文章abstract::Recent advances in immunocytochemical techniques allow the localization of specific antigens in tissue sections. The work reported here attempts to evaluate the application of antibody-labeled, disease-related protein, followed by microscopy and computerized image analysis. Using an experimental anti-tumor, polyclonal...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262338701500308
更新日期:1987-01-01 00:00:00
abstract::Helicobacter hepaticus infection induces sustained inflammation and carcinoma of the liver in A/JCr mice, and serves as a model of human cancers associated with viral hepatitis and H. pylorichronic gastritis. Here we describe the pathogenesis of premalignant disease in A/JCr mice infected with H. hepaticus. We inocula...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1080/01926230490524247
更新日期:2004-11-01 00:00:00
abstract::The prevalence of liver and skin tumors in brown bullhead ( Ameiurus nebulosus) from the Anacostia River (Washington, DC) and nearby areas was determined in 2014, 2015, and 2016. The objectives were to (1) compare tumor prevalence across space and time; (2) analyze the 1992-2016 Chesapeake Bay Tumor Database to identi...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623318823150
更新日期:2019-02-01 00:00:00
abstract::Aroclor 1254, at a dose level of 280 micrograms/kg body weight equivalent to 200 micrograms/kg/day, was given 5 days per week to rhesus monkeys over a 27 to 28 month period. Terminal clinical signs of varying severity included fingernail detachment, exuberant nail beds, weight loss, stomatitis and normocytic anemia. A...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262338601400101
更新日期:1986-01-01 00:00:00
abstract::Ribonucleic acid (RNA) interference (RNAi) is a recently discovered phenomenon whereby the introduction of double stranded (ds) RNA into the cytoplasm of the cell results in the specific and efficient degradation of complementary messenger (m) RNA and, therefore, reduced protein production. It was discovered by chance...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1080/01926230701197107
更新日期:2007-04-01 00:00:00
abstract::In organs with diverse cell populations, it is not uncommon for one type of cell to respond while others are spared. Even in an organ with common cell types, such as hepatocytes within the liver, the population of cells may respond with different sensitivities for injury or for biochemical responses to toxicants. In t...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1080/01926230252824789
更新日期:2002-01-01 00:00:00
abstract::Although the dog is a common choice among nonrodent species in evaluation of compound safety for regulatory submission, information regarding the incidence of spontaneous or incidental microscopic changes in canine peripheral nerve is limited. A retrospective examination was performed of routine histologic preparation...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623311413786
更新日期:2011-08-01 00:00:00
abstract::Determining the carcinogenic potential of materials to which humans have significant exposures is an important, complex, and imperfect exercise. Not only are the methods for such determinations protracted and expensive and use large numbers of animals, extrapolation of data from such studies to human risk is imprecise...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623309351892
更新日期:2010-01-01 00:00:00
abstract::Investigations in toxicologic neuropathology are complex undertakings because of the intricate spatial and temporal diversity in the anatomic, functional, and molecular organization of the central and peripheral nervous systems. This compilation of toxicologic neuropathology resources has been designed to consolidate ...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623310385142
更新日期:2011-01-01 00:00:00
abstract::The mechanisms underlying the development of gastrointestinal (GI) damage by the NSAIDs differ considerably from drug to drug. Aside from environmental or intersubject influence (e.g., concurrent disease, physical or sociopsychologic stress, dietary and genetic status), the intrinsic pharmacokinetic and physicochemica...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1177/019262338801600218
更新日期:1988-01-01 00:00:00
abstract::The carcinogenic potential of misoprostol, a synthetic prostaglandin E1 analogue with anti-ulcer potential, was evaluated in CD Sprague-Dawley rats. The compound was given daily by gavage at 24, 240, and 2,400 micrograms/kg, up to 150 times the daily human dose for 2 years. Necropsies were done on all animals and the ...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262338701500201
更新日期:1987-01-01 00:00:00
abstract::Cysteamine is the first chemical identified that induces acute and chronic duodenal ulcers in rodents. Structure-activity studies with cysteamine, propionitrile and their derivatives, as well as with analogues of toluene, revealed numerous alkyl and aryl duodenal ulcerogens. Among these, one of the most interesting fr...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1177/019262338801600213
更新日期:1988-01-01 00:00:00
abstract::Toxicologists and pathologists worldwide will benefit from a new, website-based, and completely searchable Nonneoplastic Lesion Atlas just released by the U.S. National Toxicology Program (NTP). The atlas is a much-needed resource with thousands of high-quality, zoomable images and diagnostic guidelines for each roden...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623313517304
更新日期:2014-01-01 00:00:00
abstract::Hepatocyte suspensions prepared from inbred male Wistar-Kyoto rats were transplanted to the livers of syngeneic recipients by injection into the portal venous system immediately after partial hepatectomy. Donor cells were labelled with 51Cr in vitro before transplantation. Animals were sacrificed 1 hr, 10 hr, 48 hr, o...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262338701500110
更新日期:1987-01-01 00:00:00
abstract::Complete evaluation of the toxicity of a new chemical entity requires critical analysis of the pattern of positive and negative findings in all types of toxicity tests, pharmacokinetics and metabolism in the species examined, and correlation of the effects with information about its pharmacodynamic and pharmacological...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339402200216
更新日期:1994-03-01 00:00:00
abstract::Oxidant generation catalyzed by metals has been postulated to account for a lung injury following exposure to air pollution particles. In particles that are predominantly carbonaceous, it is difficult to implicate such an oxidative stress as the responsible mechanism, since concentrations of metals can be extremely lo...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262330002800416
更新日期:2000-07-01 00:00:00
abstract::Specific regions in the rodent larynx exhibit cellular changes in response to inhaled xenobiotics. These regions include the base of the epiglottis, ventral pouch, and medial surfaces of the vocal processes of the arytenoid cartilages. There are interspecies differences among laboratory rodents in the microscopic anat...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339302100604
更新日期:1993-11-01 00:00:00
abstract::In standard toxicity studies, the cardiac conduction tissue is not systematically sampled and examined for histopathological changes. Most methods described use serial sectioning perpendicular to the long axis of the sinoauricular node (SAN) and atrioventricular node (AVN). Dozens of slides are needed to allow examina...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339502300107
更新日期:1995-01-01 00:00:00
abstract::A rat liver foci bioassay (RLFB) based on an initiation-promotion protocol employing preneoplastic foci of altered hepatocytes (FAH) as an endpoint, was prevalidated in 5 different laboratories. FAH were identified by immunohistochemical demonstration of glutathione-S-transferase (placental form, GSTP) and by staining...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1080/01926230390173888
更新日期:2003-01-01 00:00:00
abstract::Groups of 5 male beagle dogs were treated orally with hydralazine tablets in gelatin capsules at a dose of 12 or 24 mg/kg twice a day (6 hours apart) for 2 consecutive days. Five male dogs treated with empty gelatin capsules served as untreated controls. Clinical findings and heart rate changes during treatment and te...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262338701500404
更新日期:1987-01-01 00:00:00
abstract::Cyclodextrins are oligosaccharides which are used in the pharmaceutical industry and research as vehicles for application of apolar substances such as steroids. The aim of this study was to examine the long-term effects of parenteral administration of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) on bone. Sham-operated (SH...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623312441405
更新日期:2012-07-01 00:00:00
abstract::This is a survey of the results of recent investigations on gastrointestinal (GI) peptide hormones. In addition to the classical GI hormones (secretin, gastrin, and cholecystokinin-pancreozymin (CCK-PZ], there are at least nine other peptides whose structures and GI effects are known. These include vasoactive intestin...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1177/019262338801600203
更新日期:1988-01-01 00:00:00
abstract::The toxicity of polybrominated diphenyl ethers (PBDEs), flame-retardant components, was characterized in offspring from Wistar Han dams exposed by gavage to a PBDE mixture (DE71) starting at gestation day 6 and continuing to weaning on postnatal day (PND) 21. Offspring from the dams underwent PBDE direct dosing by gav...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623311429973
更新日期:2012-01-01 00:00:00
abstract::Colorectal cancer, responsible for 50,000 deaths per year, is a contributing factor for considerable mortalities in the United States. Consumption of well-done red meat and saturated fats rich in polycyclic aromatic hydrocarbons may be one of the causative factors for sporadic colon cancer. The objective of this study...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623309351722
更新日期:2009-12-01 00:00:00
abstract::To characterize genetic alterations occurring in renal tumorigenesis, EHEN-induced renal cell tumors were examined using restriction landmark genomic scanning (RLGS) analysis, an electrophoretic separation technique that detects gene amplifications and deletions. Comparison of DNAs from tumor against those from corres...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1080/019262301317052503
更新日期:2001-03-01 00:00:00
abstract::S-(1,2-dichlorovinyl)-L-cysteine (DCVC)-induced nephrotoxicity in vivo was investigated in New Zealand White rabbits. A primary emphasis in these studies was further characterization of DCVC-induced nephrotoxicity using a variety of serum and urinary analytes, including sodium dodecyl sulfate-polyacrylamide gel electr...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339502300405
更新日期:1995-07-01 00:00:00
abstract::Qualitative histopathology has been the gold standard for evaluation of morphological tissue changes in all organ systems, including the peripheral nervous system. However, the human eye is not sensitive enough to detect small changes in quantity or size. Peripheral nervous system toxicity can manifest as subtle chang...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623319854746
更新日期:2020-01-01 00:00:00
abstract::The coronavirus disease 2019 pandemic has affected business on numerous fronts in unprecedented and abrupt ways. From site closures and local "stay-at-home orders" to travel advisories and restrictions, the day-to-day practice of toxicologic pathology has been impacted dramatically and rapidly. A critical function of ...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623320964087
更新日期:2020-12-01 00:00:00
abstract::This overview briefly summarizes the cellular pathobiology of experimental atherosclerosis and is then followed by a consideration of how 3 major risk factors interact with the hypothesized pathogenetic process. First, since hemodynamics and blood flow influence the localization of atherosclerotic plaques, possible me...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:
更新日期:1990-01-01 00:00:00
abstract::Toxicology of the male reproductive system has received increased interest in recent years partly fuelled by the growing reports of falling sperm counts and rising reproductive disorders in the human population. Recently revised regulatory guidelines for the safety assessment of pharmaceuticals and chemicals on reprod...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1177/019262339702500201
更新日期:1997-03-01 00:00:00