Biomechanical properties of decellularized porcine pulmonary valve conduits.

Abstract:

:Tissue-engineered heart valves constructed from a xenogeneic or allogeneic decellularized matrix might overcome the disadvantages of current heart valve substitutes. One major necessity besides effective decellularization is to preserve the biomechanical properties of the valve. Native and decellularized porcine pulmonary heart valve conduits (PPVCs) (with [n = 10] or without [n = 10] cryopreservation) were compared to cryopreserved human pulmonary valve conduits (n = 7). Samples of the conduit were measured for wall thickness and underwent tensile tests. Elongation measurement was performed with a video extensometer. Decellularized PPVC showed a higher failure force both in longitudinal (+73%; P < 0.01) and transverse (+66%; P < 0.001) direction compared to human homografts. Failure force of the tissue after cryopreservation was still higher in the porcine group (longitudinal: +106%, P < 0.01; transverse: +58%, P < 0.001). In comparison to human homografts, both decellularized and decellularized cryopreserved porcine conduits showed a higher extensibility in longitudinal (decellularized: +61%, P < 0.001; decellularized + cryopreserved: +51%, P < 0.01) and transverse (decellularized: +126%, P < 0.001; decellularized + cryopreserved: +118%, P < 0.001) direction. Again, cryopreservation did not influence the biomechanical properties of the decellularized porcine matrix.

journal_name

Artif Organs

journal_title

Artificial organs

authors

Seebacher G,Grasl C,Stoiber M,Rieder E,Kasimir MT,Dunkler D,Simon P,Weigel G,Schima H

doi

10.1111/j.1525-1594.2007.00452.x

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

28-35

issue

1

eissn

0160-564X

issn

1525-1594

pii

AOR452

journal_volume

32

pub_type

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