Abstract:
:In present study, we aimed to identify angiotensin I-converting enzyme (ACE)-inhibitory peptides from a salt-free soy sauce (SFS), a newly developed antihypertensive seasoning obtained by Aspergillus oryzae fermentation of soybean in the absence of salt, which can be transported through caco-2 cell monolayers. Through an Ussing transport investigation of SFS across caco-2 cell monolayers, three di-peptides, Ala-Phe, Phe-Ile and Ile-Phe, were successfully identified from the SFS as transportable inhibitory peptides. Ala-Phe and Ile-Phe, but not Phe-Ile, exhibited ACE-inhibitory activity with IC(50) values of 165.3 microM and 65.8 microM, respectively. Kinetic studies revealed that Ile-Phe (Km: 3.1 mM, P(app): 2.4 x 10(-6) cm/s) exhibited greater affinity toward the transport compared with Ala-Phe (K(m): 48.1 mM, P(app): 1.4 x 10(-6) cm/s) and Phe-Ile (K(m): 12.7 mM, P(app): 1.4 x 10(-6) cm/s).
journal_name
Peptidesjournal_title
Peptidesauthors
Zhu XL,Watanabe K,Shiraishi K,Ueki T,Noda Y,Matsui T,Matsumoto Kdoi
10.1016/j.peptides.2007.11.006subject
Has Abstractpub_date
2008-03-01 00:00:00pages
338-44issue
3eissn
0196-9781issn
1873-5169pii
S0196-9781(07)00446-9journal_volume
29pub_type
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