Abstract：
:We have previously shown that endothelin-B receptor stimulation increases neural progenitor proliferation, partly in G(i) and extracellular matrix molecule-dependent manner. In the present study, we investigated whether G(q/11) is also involved in this response and how G(i) and G(q/11) might regulate the extracellular signal-regulated kinase (ERK) pathway and integrin signaling. Endothelin-induced ERK phosphorylation was independent of integrin ligands, and an inhibitor of G(q/11), YM-254890, as well as pertussis toxin, partially inhibited endothelin-stimulated phosphorylation of Raf-1 and ERK. Endothelin-stimulated protein kinase C (PKC) was partially inhibited by both YM-254890 and pertussis toxin, while only pertussis toxin attenuated endothelin-induced Ras activation. In contrast, endothelin increased tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin in an integrin ligand-dependent manner. Both YM-254890 and pertussis toxin partially inhibited endothelin-stimulated phosphorylation of these proteins. A PKC inhibitor and down-regulation of PKC prevented endothelin-induced phosphorylation of paxillin and ERK. In addition, endothelin-induced proliferation and DNA synthesis were partially inhibited by YM-254890 and pertussis toxin. Taken together, the results indicate that endothelin activates PKC via G(q/11) and G(i), and consequently stimulates the ERK cascade in cooperation with Ras signaling stimulated by G(i). PKC appears to increase tyrosine phosphorylation of paxillin to enhance integrin signaling, which further increases DNA synthesis and proliferation.
journal_name
Neurosci Resjournal_title
Neuroscience researchauthors
Morishita R,Ueda H,Ito H,Takasaki J,Nagata K,Asano Tdoi
10.1016/j.neures.2007.06.1478subject
Has Abstractpub_date
2007-10-01 00:00:00pages
205-14issue
2eissn
0168-0102issn
1872-8111pii
S0168-0102(07)01683-5journal_volume
59pub_type
杂志文章abstract：:Alzheimer's disease (AD) patients tend to have increased plasma levels of homocysteine. However, it is unclear whether abnormality in homocysteine levels is a primary cause of Alzheimer's disease or a disease marker. In order to investigate the relative impact of Alzheimer's disease on plasma homocysteine levels, tota...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2005.09.001
更新日期：2005-12-01 00:00:00
abstract：:Changes in synaptic efficacy at the parallel fiber (PF)-Purkinje cell (PC) synapse are postulated to be a cellular basis for motor learning. Although long-term efficacy changes lasting more than an hour at this synapse, i.e., long-term potentiation and depression, have been extensively studied, relatively short lastin...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2006.01.001
更新日期：2006-05-01 00:00:00
abstract：:A significant activity of [3H]glutamate binding was detected in homogenate particulate preparations obtained from the rat pituitary, in addition to central structures including the cerebral cortex. In contrast to the cerebral binding, the pituitary binding was significantly inhibited by Na+ ions. It was also found tha...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(86)90034-9
更新日期：1986-07-01 00:00:00
abstract：:It is still incompletely known how NMDA receptors (NMDA-R) regulate bidirectional synaptic plasticity. We examined this issue by an experimental protocol in which paired pulse stimulation (PPS) with 50ms interstimulus interval and basal frequency of 0.1Hz was applied to CA1 area of rat hippocampal slices during low Mg...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2010.02.005
更新日期：2010-06-01 00:00:00
abstract：:Methamphetamine (METH) is a psychostimulant that causes damage to dopamine (DA) axons and to non-monoaminergic neurons in the brain. The aim of the present study was to investigate short- and long-term effects of neurotoxic METH treatment on novelty-induced locomotor activity in mice. Male BALB/c mice, 12-14 weeks old...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2009.06.007
更新日期：2009-10-01 00:00:00
abstract：:We studied developmental changes in respiratory-like C4 activity and respiratory-related neurons in the ventrolateral medulla (VLM) of brainstem-spinal cord preparations from rat fetuses after embryonic day 16 (E16). In addition to respiratory nerve activity, non-respiratory activity was recorded from the C4 ventral r...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(01)00322-4
更新日期：2002-03-01 00:00:00
abstract：:Opiates have been used extensively in the treatment of pain but with the severe side effect of addiction, which is believed to be related to opiates' direct (primary) or indirect (secondary) neurotoxicity. In this study, the effects of opioids on cell growth and apoptosis have been examined in human neuroblastoma cell...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(97)00080-1
更新日期：1997-10-01 00:00:00
abstract：:Changes in the features of responses of cortical taste neurons were studied in granular insular (GI) and dysgranular insular (DI) areas of urethane-anesthetized SD rats of four age groups (4-10, 14-20, 25-35 and > 90 days). Stimuli included four basic taste stimuli, 0.1 M NH4Cl, and the water extract of a food pellet,...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(94)90082-5
更新日期：1994-06-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2014.07.006
更新日期：2014-10-01 00:00:00
abstract：:We recorded 101 neurons in the nucleus of the optic tract (NOT) of 3 rhesus monkeys. The neurons were tested in a variety of oculomotor paradigms. This report focusses on the modulation of NOT neuronal activity during smooth pursuit eye movements. A small horizontally moving spot (less than 1 degrees) elicited a direc...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(91)90103-6
更新日期：1991-10-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(98)00026-1
更新日期：1998-05-01 00:00:00
abstract：:To elucidate biophysical mechanisms underlying the Zn2+ block on the low-threshold T-type Ca2+ current (I(T)), we examined the Zn(2+)-induced alterations of gating properties of I(T) of a rat thalamic relay neuron and of alpha1G channels expressed in HEK-293 cells, using a whole-cell voltage clamp technique. The effec...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2009.12.005
更新日期：2010-04-01 00:00:00
abstract：:Pedunculopontine nucleus areas were lesioned bilaterally in the rat by local injection of kainic acid. When rats so treated were tested for active avoidance behavior in shuttle boxes we found that their avoidance acquisition was completely abolished, whereas their intertrial locomotor activity and escape behavior were...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(89)90024-2
更新日期：1989-04-01 00:00:00
abstract：:In rat hippocampal CA1 neurons recorded intracellularly from tissue slices, a rapid depolarization occurred approximately 5 min after application of ischemia-simulating medium. In extracellular recordings obtained from CA1 region, a rapid negative-going DC potential (rapid DC potential) was recorded, corresponding to ...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(99)00119-4
更新日期：2000-02-01 00:00:00
abstract：:The pattern of distribution of cholinergic fibers was examined immunohistochemically in the cat superior colliculus by using a monoclonal antibody against choline acetyltransferase (ChAT). In the superficial layers, an obvious immunoreactive zone was found in the rostral two-thirds of the outer portion of the superfic...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(89)90004-7
更新日期：1989-06-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2012.02.006
更新日期：2012-05-01 00:00:00
abstract：:Spinal cord injury (SCI) is a serious condition often affecting young and healthy individuals around the world. Electro-acupuncture (EA) has been proven to contribute towards neurologic and functional recoveries in SCI, but the underlying mechanism remains largely unknown especially regarding neural specific proteins ...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2010.04.012
更新日期：2010-08-01 00:00:00
abstract：:Properties of the Ca2+ -activated K+ current (I[K(Ca)]) were investigated in bipolar cells isolated from the goldfish retina. Pharmacological experiments and single channel current recordings demonstrated that I[K(Ca)] represented currents through BK channels, which were confined mostly to the presynaptic terminal. Th...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(97)01155-3
更新日期：1997-03-01 00:00:00
abstract：:Recent technologies and developments in neuroscience have contributed to remarkable scientific discoveries, and have also raised many new philosophical, ethical, legal, and social issues. Research in "neuroethics" has identified various ethical issues, which will be difficult for current biomedical ethics to resolve f...
journal_title：Neuroscience research
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.neures.2006.09.004
更新日期：2007-01-01 00:00:00
abstract：:Intrastriatal hemorrhage in rats causes neurodegenaration of the substantia nigra (SN) followed by the appearance of ED1(+) cells (macrophage/microglia). ED1(+) cells were observed for at least 8 weeks after hemorrhage. Phosphorylation of p38 mitogen-activated protein kinase (MAPK) was shown in ED1(+) cells with the e...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(03)00065-8
更新日期：2003-07-01 00:00:00
abstract：:The cerebellar Purkinje cell has highly branched dendrites extending in a transverse plane to the cerebellar folium. Branching patterns of the dendrites were subjected to fractal analysis to obtain a quantitative measure of their intricacy. Using a square-covering method, we found that dendrites have a statistical sel...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(92)90031-7
更新日期：1992-02-01 00:00:00
abstract：:In the present study we measured cardiovascular and respiratory reflexes evoked by administration of bradykinin and capsaicin into the hindlimb vasculature of anaesthetised rats, whilst simultaneously recording activity of sensory afferents on the adventitial surface of femoral arteries and veins. Bradykinin (0.9 nmol...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2004.07.006
更新日期：2004-11-01 00:00:00
abstract：:Confocal laser scanning microscopy (CLSM) was used to record spatial and dynamic changes in the intracellular Ca2+ [(Ca2+]i) of bullfrog sympathetic ganglion cells in excised tissue or in culture. A CLSM utilizing Ar ion laser (488 nm) and recording fluo-3 fluorescence yielded the sliced image of ganglion cells, while...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(91)90082-a
更新日期：1991-05-01 00:00:00
abstract：:Over the last few decades, motor imagery has attracted the attention of researchers as a prototypical example of 'embodied cognition' and also as a basis for neuro-rehabilitation and brain-machine interfaces. The current definition of motor imagery is widely accepted, but it is important to note that various abilities...
journal_title：Neuroscience research
pub_type： 杂志文章,评审
doi：10.1016/j.neures.2015.11.003
更新日期：2016-03-01 00:00:00
abstract：:Fifteen rabbits were classically conditioned using stimulation of the right pontine nuclei as a conditioned stimulus and a corneal airpuff as an unconditioned stimulus. After conditioning criterion was reached, the stimulation conditioned stimulus was transferred to the left pontine nuclei and the rate of conditioned ...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(86)90057-x
更新日期：1986-09-01 00:00:00
abstract：:We investigated brain activity in 3-5-year-old preschoolers as they listened to connected speech stimuli in Japanese (first language), English (second language), and Chinese (a rarely exposed, foreign language) using near-infrared spectroscopy. Unlike the younger preschoolers who had been exposed to English for almost...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2012.02.004
更新日期：2012-05-01 00:00:00
abstract：:Gene targeting to disrupt gene expression in a temporal and spatial manner in a specific tissue using Cre recombinase-mediated gene inactivation has been proven to be useful to study in vivo gene function. To delete genes specifically in neurons during the late phase of brain development, we have generated transgenic ...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(01)00216-4
更新日期：2001-06-01 00:00:00
abstract：:Following neonatal hypoxia-ischemia (HI) serotonin (5-hydroxytryptamine, 5-HT) levels are decreased in the brain. The regulation of brain 5-HT is dependent on the serotonin transporter (SERT) localised at the neuronal pre-synaptic cell membrane. However SERT can also traffic away from the cell membrane into the cytoso...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2012.04.002
更新日期：2012-07-01 00:00:00
abstract：:Recently the authors showed that a computational model of visual saliency could account for changes in gaze behavior of monkeys with damage in the primary visual cortex. Here we propose a neural prosthesis to restore eye gaze behavior by electrically stimulating the superior colliculus to drive visual attention. The s...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2013.07.007
更新日期：2014-01-01 00:00:00
abstract：:Although neuromuscular blocking agents (NMBAs) function as muscular nicotinic acetylcholine receptor (nAChR) antagonists, several studies have shown that they block neuronal nAChRs as well, which led us to hypothesize that these agents can affect neuronal nAChRs expressed in respiratory centers. To test this hypothesi...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(03)00213-x
更新日期：2003-11-01 00:00:00
