Abstract：
OBJECTIVE:Pigment epithelial-derived factor (PEDF) is a potent anti-angiogenic factor whose effects are partially mediated through the induction of endothelial cell apoptosis. The pathway mediating endothelial cell apoptosis has not been fully established. Here we investigated the participation of peroxisome proliferator-activated receptor gamma (PPARgamma) and p53 in the apoptosis of human umbilical vein endothelial cells (HUVECs). METHODS AND RESULTS:HUVECs pretreated with either PPARgamma antagonist or PPARgamma small interfering RNA (siRNA) suppressed PEDF-induced apoptosis as determined by TUNEL assay, annexin V-FITC/PI staining, and cleavage of procaspase-8, -9, -3. PEDF sequentially induced PPARgamma and p53 expression as observed in immunoblotting and immunofluoresence assays. PEDF also increased the transcriptional activity of PPARgamma as evident from electromobility shift assays, and p53 as determined by the phosphorylation and acetylation of p53 and the induction of Bax. The induction of p53 by PEDF was abolished by either PPARgamma antagonist or PPARgamma siRNA. PEDF-mediated HUVEC apoptosis and cleavage of procaspases were significantly attenuated by p53 siRNA. CONCLUSIONS:Our observations indicate that PEDF induces HUVECs apoptosis through the sequential induction of PPARgamma and p53 overexpression. With the growing interest in anti-angiogenesis as a novel approach to cancer therapy, defining the mechanism of PEDF-mediated HUVEC apoptosis may facilitate the development of new therapeutics.
journal_name
Cardiovasc Resjournal_title
Cardiovascular researchauthors
Ho TC,Chen SL,Yang YC,Liao CL,Cheng HC,Tsao YPdoi
10.1016/j.cardiores.2007.06.032subject
Has Abstractpub_date
2007-11-01 00:00:00pages
213-23issue
2eissn
0008-6363issn
1755-3245pii
S0008-6363(07)00311-2journal_volume
76pub_type
杂志文章abstract：OBJECTIVE:Transient atrial fibrillation is sometimes observed following adenosine administration and adenosine is known to shorten atrial action potential duration and refractory period. This study was designed to characterise the dose-response relationship of adenosine on these variables relative to arrhythmia inducti...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/28.9.1385
更新日期：1994-09-01 00:00:00
abstract：:Mechanical stress signals transmitted through the heart walls during hemodynamic loading are sensed by the myocytes, which respond with changes in contractile performance and gene expression. External forces play an important role in physiological heart development and hypertrophy, but disruption of the well-balanced ...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/j.cardiores.2007.03.029
更新日期：2008-03-01 00:00:00
abstract：AIMS:Regional heterogeneities in contraction contribute to heart failure with reduced ejection fraction (HFrEF). We aimed to determine whether regional changes in myocardial relaxation similarly contribute to diastolic dysfunction in post-infarction HFrEF, and to elucidate the underlying mechanisms. METHODS AND RESULT...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvy257
更新日期：2019-03-15 00:00:00
abstract：:The cardiac mechano- and chemoreceptors are broadly distributed in the myocardium and coronary vessels. A portion of these receptors extends over the epicardium and pericardium and therefore can be excited by mechanical or chemical stimuli directly applied to the surface of the heart. Excitation of epicardial receptor...
journal_title：Cardiovascular research
pub_type： 评论,杂志文章,评审
doi：10.1016/s0008-6363(99)00319-3
更新日期：2000-01-01 00:00:00
abstract：OBJECTIVE:The aim was to determine the relationship between the rate of reflow and vulnerability to reperfusion induced arrhythmias. METHODS:Isolated rat hearts, in which left and right coronary arteries were perfused independently, were subjected to transient (10 min) cessation of flow to the left coronary bed. Durin...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/26.4.316
更新日期：1992-04-01 00:00:00
abstract：AIMS:The lysophospholipid mediator sphingosine-1-phosphate (S1P) acts on vascular endothelial cells to stimulate migration, proliferation, and capillary-like tube formation in vitro. It is unknown whether S1P stimulates in vivo angiogenesis induced under tissue ischaemia. We investigated the effects of both exogenously...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvn002
更新日期：2008-05-01 00:00:00
abstract：:Cardiomyocytes express a surprisingly large number of potassium channel types. The primary physiological functions of the currents conducted by these channels are to maintain the resting membrane potential and mediate action potential repolarization under basal conditions and in response to changes in the concentratio...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvaa068
更新日期：2020-07-15 00:00:00
abstract：OBJECTIVE:Matrix metalloproteinases (MMPs) cause extracellular matrix degradation and may be involved in the rupture of atherosclerotic plaques by degrading fibrous cap, resulting in the intravascular thrombus formation. Here we examined whether local overexpression of MMP-9 alters the characteristics of arteriosclerot...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(02)00710-1
更新日期：2003-02-01 00:00:00
abstract：AIMS:Our aim was to determine whether the repeated, binge administration of 3,4-methylenedioxymethamphetamine (ecstasy; MDMA) produces structural and/or functional changes in the myocardium that are associated with oxidative stress. METHODS AND RESULTS:Echocardiography and pressure-volume conductance catheters were us...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvn129
更新日期：2008-09-01 00:00:00
abstract：OBJECTIVE:Inhibitors of poly (ADP-ribose) synthetase (PARS) activity reduce the infarct size caused by regional myocardial ischaemia and reperfusion in the rabbit and rat in vivo. The mechanism of action of these inhibitors is unclear. Here we investigate the effects of the PARS inhibitor 3-aminobenzamide (3-AB) on inf...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(98)00221-1
更新日期：1999-01-01 00:00:00
abstract：:Atherosclerosis is considered to be a form of chronic inflammation governed by a complex network of inter- and intra-cellular signaling pathways. The pleiotropic cytokine interferon-gamma (IFN-gamma) is a key pro-inflammatory mediator that is expressed at high levels in atherosclerotic lesions. IFN-gamma regulates the...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/j.cardiores.2005.04.019
更新日期：2005-07-01 00:00:00
abstract：:By measuring the coronary sinus blood flow using the thermodilution technique the influence of "thermodilution catheter" withdrawal from the great cardiac vein to the ostium of the coronary sinus was investigated in 41 patients. In addition, the influence of normal and forced respiration on coronary sinus blood flow w...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/19.1.27
更新日期：1985-01-01 00:00:00
abstract：AIMS:Vascular calcification often occurs with advancing age, atherosclerosis, and metabolic disorders such as diabetes mellitus and end-stage renal disease. Vascular calcification is associated with cardiovascular events and increased mortality. Nitric oxide (NO) is crucial for maintaining vascular function, but little...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvm049
更新日期：2008-01-01 00:00:00
abstract：Aims:Endothelial hyperpermeability exacerbates multiple organ damage during inflammation or infection. The endothelial glycocalyx, a protective matrix covering the luminal surface of endothelial cells (ECs), undergoes enzymatic shedding during inflammation, contributing to barrier hyperpermeability. A disintegrin and m...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvy167
更新日期：2018-11-01 00:00:00
abstract：:To evaluate the effect of beta blockade on energy metabolism in ischaemic myocardium metoprolol tartrate in doses of 0.2 or 0.5 mg.kg-1 or saline was injected into 24 anaesthetised dogs 20 min after coronary ligation. Regional myocardial blood flow was measured by the hydrogen gas clearance method, and the heart rate ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/21.9.660
更新日期：1987-09-01 00:00:00
abstract：OBJECTIVE:The aims of the study were (1) to elucidate the effects of raised oxygen tension on the response of the ductus arteriosus to a range of vasodilators; and (2) to establish the effect, if any, of cyclo-oxygenase inhibition on the interaction between oxygen and prostaglandin (PG)E2. METHODS:Rings of ductus arte...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/27.12.2205
更新日期：1993-12-01 00:00:00
abstract：OBJECTIVE:Ischaemic preconditioning may be mediated by oxygen free radicals generated during preconditioning. Conflicting results have been reported regarding the effect of superoxide dismutase (SOD) in attenuating the cardioprotective effect of preconditioning. The aim of the study was to reconcile this conflict by ex...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/28.7.980
更新日期：1994-07-01 00:00:00
abstract：AIMS:We analysed the possible protective role of human endothelial (EPCs) and smooth muscle (SPCs) progenitor cells on atherosclerosis development in apoE(-/-)RAG2(-/-) mice. We determined plasma levels of SPCs in coronary patients. METHODS AND RESULTS:ApoE(-/-)RAG2(-/-) mice received four intravenous injections of sa...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvm034
更新日期：2008-02-01 00:00:00
abstract：AIMS:Following a large myocardial infarction (MI), remaining viable muscle often undergoes pathological remodelling and progresses towards chronic heart failure. Mitochondria may also be affected by this process and, due to their functional importance, likely contribute to the progression of the disease. Aerobic interv...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvt080
更新日期：2013-07-01 00:00:00
abstract：OBJECTIVE:The aim was to examine whether an absolute reduction in energy supply is required for preconditioning against myocardial infarction, and whether an episode of increased adrenergic activity with or without supply-demand imbalance is capable of triggering cardioprotection. METHODS:41 anaesthetised dogs were su...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/27.11.2071
更新日期：1993-11-01 00:00:00
abstract：AIMS:Vascular remodelling and aortic aneurysm formation are induced mainly by inflammatory responses in the adventitia and media. However, relatively little is known about the mechanistic significance of endothelium in the pathogenesis of these vascular disorders. The transcription factor nuclear factor-kappa B (NF-κB)...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvs298
更新日期：2013-01-01 00:00:00
abstract：OBJECTIVE:We tested the hypothesis that simultaneous inhibition of the endothelial integrin alpha(v)beta(3) and the platelet glycoprotein IIb/IIIa receptor will substantially reduce infarct size in a model of acute coronary thrombosis and primary angioplasty. METHODS:Dogs were subjected to thrombus formation in the le...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2005.01.016
更新日期：2005-06-01 00:00:00
abstract：:The effects of both gradual and abrupt coronary occlusion on regional wall function (sonomicrometry) and left ventricular relaxation were studied in the intact dog heart. The ischaemic dysfunction observed in the two interventions as assessed by pressure-length loops showed considerably different patterns. The regiona...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/21.7.507
更新日期：1987-07-01 00:00:00
abstract：:The effects of L-propionylcarnitine on the recovery of cardiac contractile performance after global ischaemia and reperfusion were studied in isolated perfused rat hearts. The addition of either 5.5 or 11 mmol X litre-1 L-propionylcarnitine significantly improved the recovery of cardiac output, left ventricular pressu...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/20.7.536
更新日期：1986-07-01 00:00:00
abstract：:The effects on an acute myocardial infarction of equipotent hypotensive doses of nitroglycerin and dipyridamole were studied using the thermographic technique in open-chest dogs. Nitroglycerin did not reduce the cool ischaemic area in any of the nine dogs studied. In six of the dogs, in which the ischaemic region coul...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/13.5.281
更新日期：1979-05-01 00:00:00
abstract：OBJECTIVES:To investigate the role of ventricular and atrial beta-adrenoceptor activation by isoprenaline in the genesis of rhythm disorders and risk of fibrillation in the healthy or ischaemic heart. METHODS:The study was performed in anaesthetized, open-chest pigs. Electrical fibrillation threshold (EFT) of the vent...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(96)00156-3
更新日期：1996-12-01 00:00:00
abstract：:The heart is challenged by a plethora of extracellular stimuli over the course of a normal day, each of which distinctly influences myocardial contractile function. It is therefore not surprising that myocardial metabolism also oscillates in a time-of-day dependent manner. What is becoming increasingly apparent is tha...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1093/cvr/cvn054
更新日期：2008-07-15 00:00:00
abstract：Aims:Circulating proteins larger than 3 nm can be transported across continuous endothelial barrier of blood vessels via transcytosis. However, excessive accumulation of serum proteins within the vessel walls is uncommon even for those abundant in the circulation. The aim of this study was to investigate how transcytos...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvy181
更新日期：2019-02-01 00:00:00
abstract：OBJECTIVES:Angiotensin converting enzyme (ACE) activity in the plasma does not change significantly with hypertension in two-kidney, one-clip hypertensive (2K-1C) rats. However, heart ACE activity and mRNA increase with hypertension. We measured the ACE activity and mRNA in hypertrophied hearts at different times after...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：OBJECTIVES:Nitric oxide (NO) has complex effects on myocardial function particularly following ischaemia-reperfusion. The goal of this study was to examine the result of repetitive myocardial stunning on myocardial NO release and expression of inducible (iNOS) and constitutive (eNOS) NO synthases. METHODS AND RESULTS:...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(99)00149-2
更新日期：1999-08-15 00:00:00
