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Superoxide dismutase and N-2-mercaptopropionyl glycine attenuate infarct size limitation effect of ischaemic preconditioning in the rabbit.

Abstract：

OBJECTIVE:Ischaemic preconditioning may be mediated by oxygen free radicals generated during preconditioning. Conflicting results have been reported regarding the effect of superoxide dismutase (SOD) in attenuating the cardioprotective effect of preconditioning. The aim of the study was to reconcile this conflict by examining the effect of three different oxyradical scavengers on the infarct size limiting effect of preconditioning. METHODS:Anaesthetised open chest rabbits were subjected to 30 min coronary occlusion and 48 h reperfusion. In the preconditioning groups, rabbits were subjected to a single 5 min occlusion and 5 min reperfusion before 30 min sustained ischaemia. In these groups, the oxyradical scavengers SOD (15,000 U.kg-1), N-2-mercaptopropionyl glycine (MPG, 20 mg.kg-1), and dimethylthiourea (DMTU, 500 mg.kg-1), or placebo saline, were infused before and during preconditioning. In the non-preconditioning groups, these agents were given in the same time frame before 30 min of ischaemia. After 2 d reperfusion, infarct size was measured microscopically. RESULTS:In the saline treated controls, preconditioning markedly limited microscopical infarct size (percent of area at risk): 13(SEM 3)% (n = 9) v 49(9)% (n = 8), p < 0.05. Treatment of the preconditioning groups with SOD or MPG attenuated this cardioprotection [infarct size 31(5)% (n = 11) and 42(8)% (n = 11), respectively, p < 0.05 v the saline treated preconditioning group], but treatment with DMTU did not [infarct size 23(6)% (n = 11), p = NS v the saline treated preconditioning group]. In the non-preconditioning groups, none of the treatments modified infarct size: 50(9)% (n = 7), 56(5)% (n = 8), and 61(6)%, (n = 8), respectively, p = NS v saline treated control. CONCLUSIONS:Cardioprotection by preconditioning is mediated, at least in part, by oxyradicals which are scavenged by SOD or MPG in rabbits.

journal_name

Cardiovasc Res

journal_title

Cardiovascular research

authors

Tanaka M,Fujiwara H,Yamasaki K,Sasayama S

doi

10.1093/cvr/28.7.980

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

980-6

issue

eissn

0008-6363

issn

1755-3245

pii

0008-6363(94)90263-1

journal_volume

pub_type

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Superoxide dismutase and N-2-mercaptopropionyl glycine attenuate infarct size limitation effect of ischaemic preconditioning in the rabbit.