Growth hormone (GH) receptor isoform in acromegaly: lower concentrations of GH but not insulin-like growth factor-1 in patients with a genomic deletion of exon 3 in the GH receptor gene.

abstract：BACKGROUND:Extracellular vesicles (EVs) contain a rich cargo of different RNA species with specialized functions and clinical applications. However, the landscape and characteristics of extracellular vesicle long RNA (exLR) in human blood remain largely unknown. METHODS:We presented an optimized strategy for exLR sequ...

journal_title：Clinical chemistry

authors： Li Y,Zhao J,Yu S,Wang Z,He X,Su Y,Guo T,Sheng H,Chen J,Zheng Q,Li Y,Guo W,Cai X,Shi G,Wu J,Wang L,Wang P,He X,Huang S

更新日期：2019-06-01 00:00:00

abstract：BACKGROUND:Immunoassays specific for 1-84 parathyroid hormone (PTH) reportedly reflect the bioactivity of PTH; however, PTH immunoassays can be susceptible to interference by cross-reacting PTH fragments. In addition, these assays currently lack standardization. A methodology using immunocapture purification with liqui...

journal_title：Clinical chemistry

authors： Kumar V,Barnidge DR,Chen LS,Twentyman JM,Cradic KW,Grebe SK,Singh RJ

更新日期：2010-02-01 00:00:00

abstract：BACKGROUND:Massively parallel sequencing has recently been used in noninvasive prenatal diagnosis. The current costs of this technology are still relatively expensive, however, and sample throughput is still relatively low when it is used as a molecular diagnostic tool. Rather than nonselectively sequencing the genome,...

journal_title：Clinical chemistry

authors： Liao GJ,Lun FM,Zheng YW,Chan KC,Leung TY,Lau TK,Chiu RW,Lo YM

更新日期：2011-01-01 00:00:00

abstract：:In the diagnosis of chronic (as opposed to acute) liver diseases, combinations of indicators are needed to improve specificity. Alanine aminopeptidase (AAP; microsomal aminopeptidase, EC 3.4.11.2) activity in serum reportedly is a very sensitive indicator of intrahepatic cholestasis and biliary obstruction; it is also...

journal_title：Clinical chemistry

authors： Mueller PW,Phillips DL,Steinberg KK

更新日期：1987-03-01 00:00:00

abstract：:The fructosamine test is considered clinically useful for assessing short-term integrated control of blood glucose, but there are few published data to support this hypothesis. We fractionated glycated and nonglycated proteins by affinity chromatography on phenylboronate columns and, with specific immunochemical metho...

journal_title：Clinical chemistry

authors： Zoppi F,Mosca A,Granata S,Montalbetti N

更新日期：1987-10-01 00:00:00

abstract：:In this new method for determining urinary protein, the reaction is complete within 10 min at 37 degrees C. This method is applicable to automated as well as manual measurements. Protein concentration and absorbance at 600 nm are linearly related throughout a wide range of concentrations, 10 to 16 000 mg/L. However, t...

journal_title：Clinical chemistry

authors： Watanabe N,Kamei S,Ohkubo A,Yamanaka M,Ohsawa S,Makino K,Tokuda K

更新日期：1986-08-01 00:00:00

abstract：:We examined the variability of lipid and lipoprotein concentrations in plasma from a population sample from the Jerusalem Lipid Research Clinic study. Coefficients of variation of about 8% for plasma cholesterol, 11% to 15% for low- and high-density-lipoprotein cholesterol, and about 30% for triglyceride were reported...

journal_title：Clinical chemistry

authors： Friedlander Y,Kark JD,Stein Y

更新日期：1985-07-01 00:00:00

abstract：BACKGROUND:Eph receptors and their ligands, the ephrins, represent a large class of cell-cell communication molecules with well-defined developmental functions. Their role in healthy adult tissues and in human disease is still largely unknown, although diverse roles in carcinogenesis have been postulated. METHODS:We e...

journal_title：Clinical chemistry

authors： Hafner C,Schmitz G,Meyer S,Bataille F,Hau P,Langmann T,Dietmaier W,Landthaler M,Vogt T

更新日期：2004-03-01 00:00:00

abstract：BACKGROUND:Urinary cross-linked N-telopeptide of type I collagen (NTX) is a reliable bone resorption marker in patients with metabolic bone disease. We assessed a clinically available serum NTX assay suitable for anuric patients on hemodialysis (HD). METHODS:Serum concentrations of NTX, C-terminal telopeptide of type ...

journal_title：Clinical chemistry

authors： Maeno Y,Inaba M,Okuno S,Yamakawa T,Ishimura E,Nishizawa Y

更新日期：2005-12-01 00:00:00

abstract：BACKGROUND:Testing for troponin has important clinical value for patients who present with typical symptoms of acute coronary syndromes (ACS) such as chest pain (CP). Much less is known about the value of troponin testing for patients who present with other symptoms of ACS (anginal equivalent symptoms). METHODS:The ut...

journal_title：Clinical chemistry

authors： Ritter D,Lee PA,Taylor JF,Hsu L,Cohen JD,Chung HD,Virgo KS

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:The time required for bloodstream pathogen detection, identification (ID), and antimicrobial susceptibility testing (AST) does not satisfy the acute needs of disease management. Conventional methods take up to 3 days for ID and AST. Molecular diagnostics have reduced times for ID, but their promise to suppla...

journal_title：Clinical chemistry

authors： Andini N,Hu A,Zhou L,Cogill S,Wang TH,Wittwer CT,Yang S

更新日期：2018-10-01 00:00:00

abstract：:Single-strand conformation polymorphism (SSCP) analysis is widely used to prescreen mutations in p53 gene. However, standardization of SSCP to detect p53 mutations has rarely been pursued so far. We have developed complete conditions for a temperature-controlled nonradioactive SSCP for mutation detection in amplified ...

journal_title：Clinical chemistry

authors： Welsh JA,Castrén K,Vähäkangas KH

更新日期：1997-12-01 00:00:00

abstract：:We describe a simple method in which a water-soluble mixture of triocatanoin and a surfactant, "Triton X-114," are used in preparing solutions of triglycerides (triacylglycerols) in either human serum, solutions of albumin, or water. Analytical recovery added triglyceride was quantitative by two methods. The addition ...

journal_title：Clinical chemistry

authors： Bonderman DP,Proksch GJ,Bonderman PW

更新日期：1976-08-01 00:00:00

abstract：:I present the general pattern relationship between (a) plasma fibrinogen and cholinesterase activity and (b) plasma fibrinogen and hemoglobin, in 250 and 310 hospitalized adults, respectively. Although responses of cholinesterase were often unrelated to fibrinogen, when its activity in plasma was depressed, above-norm...

journal_title：Clinical chemistry

authors： Rice EW

更新日期：1977-01-01 00:00:00

abstract：:We describe a modification of the MacGee method [Anal. Chem. 42, 421 (1970)] for rapid determination of phenytoin (diphenylhydantoin) in plasma by gas-liquid chromatography, in which an ethylaing reagent is used instead of the more common methylating reagents. With this modification, several N-methylated antiepileptic...

journal_title：Clinical chemistry

authors： Friel P,Troupin AS

更新日期：1975-05-01 00:00:00

abstract：:For many lysosomal storage disorders, presymptomatic detection, before the onset of irreversible pathology, will greatly improve the efficacy of current and proposed therapies. In the absence of a family history, presymptomatic detection can be achieved only by a comprehensive newborn screening program. Recently we re...

journal_title：Clinical chemistry

authors： Hua CT,Hopwood JJ,Carlsson SR,Harris RJ,Meikle PJ

更新日期：1998-10-01 00:00:00

abstract：BACKGROUND:The optimal situation in external quality assessment (EQA) is to use commutable materials. No previous study has examined the commutability of a whole-blood material for point-of-care (POC) testing. The aim of this study was to determine the commutability of the Norwegian Quality Improvement of Laboratory Ex...

journal_title：Clinical chemistry

authors： Bukve T,Sandberg S,Vie WS,Sølvik U,Christensen NG,Stavelin A

更新日期：2019-06-01 00:00:00

abstract：BACKGROUND:A unique requirement for the molecular diagnosis of mitochondrial DNA (mtDNA) disorders is the ability to detect heteroplasmic mtDNA mutations and to distinguish them from homoplasmic sequence variations before further testing (e.g., sequencing) is performed. We evaluated the potential utility of temporal te...

journal_title：Clinical chemistry

authors： Chen TJ,Boles RG,Wong LJ

更新日期：1999-08-01 00:00:00

abstract：:Two-dimensional electroimmunodiffusion is rarely used in clinical chemistry, because its potentialities are not yet widely appreciated. Studying mouse anaphylaxis, we observed with this technique certain striking short- and long-term pathologic changes in several important serum antigens, which aptly demonstrate its u...

journal_title：Clinical chemistry

authors： Quintero S,Crowle AJ

更新日期：1979-05-01 00:00:00

abstract：:We evaluated the enzymic mechanism by which 3-keto butylidene-beta-2-chloro-4-nitrophenyl maltopentaoside (3KB-G5-CNP) serves as a substrate for serum pancreatic (p-) and salivary (s-) amylases. In aliquots of the reaction mixture, three kinds of beta-2-chloro-4-nitrophenyl oligosaccharides (glucose, maltoside, and ma...

journal_title：Clinical chemistry

authors： Abe A,Nishimura T,Noma A,Hamano K

更新日期：1991-08-01 00:00:00

abstract：BACKGROUND:Optimal cutoff values for tests results involving continuous variables are often derived in a data-driven way. This approach, however, may lead to overly optimistic measures of diagnostic accuracy. We evaluated the magnitude of the bias in sensitivity and specificity associated with data-driven selection of ...

journal_title：Clinical chemistry

authors： Leeflang MM,Moons KG,Reitsma JB,Zwinderman AH

更新日期：2008-04-01 00:00:00

abstract：BACKGROUND:Recent use of Bacillus anthracis spores as a bioweapon has highlighted the need for a continuous monitoring system. Current monitoring systems rely on antibody-derived probes, which are not hardy enough to withstand long-term use under extreme conditions. We describe new, phage-derived probes that can be use...

journal_title：Clinical chemistry

authors： Brigati J,Williams DD,Sorokulova IB,Nanduri V,Chen IH,Turnbough CL Jr,Petrenko VA

更新日期：2004-10-01 00:00:00

abstract：BACKGROUND:Quantitative, miniaturized nucleic acid assays and immunoassays can be developed with single microparticles, microfluorometric detection, and intrinsically fluorescent lanthanide chelates in a multiple assay format to decrease reagent consumption, cost, and assay time. We used recombinant Fab fragments to ca...

journal_title：Clinical chemistry

authors： Härmä H,Tarkkinen P,Soukka T,Lövgren T

更新日期：2000-11-01 00:00:00

abstract：:To see if saliva is a valid substitute for plasma in assay of progesterone even when concentrations of hormone and binding proteins are fluctuating, we determined the concentrations of total and free progesterone in plasma and salivary progesterone in specimens from 36 women volunteers during the course of pregnancy a...

journal_title：Clinical chemistry

authors： Meulenberg PM,Hofman JA

更新日期：1989-01-01 00:00:00

abstract：:Human myocardial creatine kinase isoenzyme MM is present as a single form in tissue, but upon its release into plasma two additional forms, with faster anodal migration, are apparent on polyacrylamide electrophoresis. We designate the three forms as MM3, MM2, and MM1 in increasing order of anodal mobility. When tissue...

journal_title：Clinical chemistry

authors： Perryman MB,Knell JD,Roberts R

更新日期：1984-05-01 00:00:00

abstract：BACKGROUND:Since its beginnings, newborn screening for cystic fibrosis (CF) using an assay for immunoreactive trypsinogen (IRT) has been plagued by a high rate of false-positive results (screen positive, diagnosis negative), despite attempts to reduce this rate by use of altered cutoffs and second-tier DNA testing. IRT...

journal_title：Clinical chemistry

authors： Lindau-Shepard BA,Pass KA

更新日期：2010-03-01 00:00:00

abstract：:To evaluate their potential usefulness in the differential diagnosis of Cushing's syndrome, we estimated the urinary excretion rates of the following non-metabolized, unbound steroid hormones: pregnenolone, progesterone, 17-OH-pregnenolone, 17-OH-progesterone, dehydroepiandrosterone (DHEA), androstenedione, testostero...

journal_title：Clinical chemistry

authors： Schöneshöfer M,Weber B,Oelkers W,Nahoul K,Mantero F

更新日期：1986-01-01 00:00:00

abstract：:We describe estimation of total cortisol in serum by HPLC. Incorporation of a new technique to totally automate sample preparation decreases costs. The benefits of HPLC ensure that interferences from other steroids are avoided. The method can also be used to estimate concentrations of other glucocorticoids in serum. ...

journal_title：Clinical chemistry

authors： Turnell DC,Cooper JD,Green B,Hughes G,Wright DJ

更新日期：1988-09-01 00:00:00

abstract：BACKGROUND:Galectin-3 has emerged as a potential useful novel biomarker for heart failure and cardiovascular disease (CVD). However, it remains unclear whether galectin-3 is associated with recurrent cardiovascular events during long-term follow-up of patients with stable coronary heart disease (CHD) after adjustment f...

journal_title：Clinical chemistry

authors： Jansen H,Koenig W,Jaensch A,Mons U,Breitling LP,Scharnagl H,Stojakovic T,Schunkert H,Brenner H,Rothenbacher D

更新日期：2016-10-01 00:00:00

abstract：:Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is a recessively inherited defect in the mitochondrial beta-oxidation of fatty acids. A single nucleotide change, the A985 --> G transition, in the MCAD gene accounts for approximately 90% of all the disease-causing mutations in the patients. We have used PCR to am...

journal_title：Clinical chemistry

authors： Romppanen EL,Mononen T,Mononen I

更新日期：1998-01-01 00:00:00