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Characterization of DNA methylation change in stem cell marker genes during differentiation of human embryonic stem cells.

Abstract：

:Pluripotent human embryonic stem cells (hESCs) have the distinguishing feature of innate capacity to allow indefinite self-renewal. This attribute continues until specific constraints or restrictions, such as DNA methylation, are imposed on the genome, usually accompanied by differentiation. With the aim of utilizing DNA methylation as a sign of early differentiation, we probed the genomic regions of hESCs, particularly focusing on stem cell marker (SCM) genes to identify regulatory sequences that display differentiation-sensitive alterations in DNA methylation. We show that the promoter regions of OCT4 and NANOG, but not SOX2, REX1 and FOXD3, undergo significant methylation during hESCs differentiation in which SCM genes are substantially repressed. Thus, following exposure to differentiation stimuli, OCT4 and NANOG gene loci are modified relatively rapidly by DNA methylation. Accordingly, we propose that the DNA methylation states of OCT4 and NANOG sequences may be utilized as barometers to determine the extent of hESC differentiation.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Yeo S,Jeong S,Kim J,Han JS,Han YM,Kang YK

doi

10.1016/j.bbrc.2007.05.120

subject

Has Abstract

pub_date

2007-08-03 00:00:00

pages

536-42

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)01099-6

journal_volume

359

pub_type

杂志文章

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