Abstract:
:To study the tumor cell autonomous processes of metastasis, an in vivo tumor metastasis model is required that excludes the involvement of the innate immune system. For this purpose we used the established syngeneic MTLn3 cell - Fischer 344 tumor model. MTLn3 cells are efficiently eradicated by NK cells in vivo. Using isolated cell systems, we provide evidence for apoptosis-induction by IL-2 activated NK cells, but not T-cells, despite the expression of MHC class I. This is largely mediated by the perforin/granzyme B pathway in MTLn3 cells in a caspase-dependent manner. Temporal in vivo depletion of NK cells by an antibody-based method, dramatically improved colonization of the lungs by MTLn3 cells, from 5 metastases in the untreated animals to 130 metastases in the NK-depleted animals. Thus, we improved the syngeneic MTLn3-Fischer 344 tumor model by temporal depletion of NK cells of which the advantages over the use of immunodeficient animals are evident.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
van Nimwegen MJ,Verkoeijen S,Kuppen PJ,Velthuis JH,van de Water Bdoi
10.1007/s10585-007-9075-9subject
Has Abstractpub_date
2007-01-01 00:00:00pages
379-87issue
5eissn
0262-0898issn
1573-7276journal_volume
24pub_type
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pub_type: 杂志文章,多中心研究
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章,评审
doi:10.1023/a:1015531321445
更新日期:2002-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1006715221088
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更新日期:2013-04-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF00736406
更新日期:1983-07-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1006568103588
更新日期:1998-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-005-7889-x
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pub_type: 杂志文章
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