Abstract:
:In GH4C1 cells, membrane depolarization induces a rapid and sustained increase in the cytosolic free calcium concentration ([Ca2+]i). In the present study we have investigated the role of [Ca2+]i in the regulation of basal intracellular pH (pHi). Depolarizing GH4C1 cells in buffer containing 0.4 mM extracellular Ca2+ decreased basal pHi from 7.02 +/- 0.04 to 6.85 +/- 0.03 (P less than 0.05). If the depolarization-induced influx of Ca2+ was inhibited by chelating extracellular Ca2+ or blocking influx through voltage-operated Ca2+ channels with nimodipine, no acidification was observed. Addition of TRH induced a rapid activation of Na+/H+ exchange in acidified cells, increasing pHi by 0.14 +/- 0.03 U. The action of TRH was blunted if extracellular Ca2+ was chelated; however, if influx of Ca2+ via voltage-operated channels was blocked by nimodipine, TRH still increased pHi. To deplete ATP, we incubated cells with 2-deoxy-D-glucose for 15-20 min and observed a decrease in basal pHi to 6.75 +/- 0.03 (P less than 0.05). No additional acidification was obtained when 2-deoxy-D-glucose-treated cells were depolarized, and no TRH-induced activation of Na+/H+ exchange was observed. Addition of ionomycin or 12-O-tetradecanoyl-phorbol-13-acetate separately to acidified cells had only modest effects on pHi; however, addition of 12-O-tetradecanoyl-phorbol-13-acetate and ionomycin together increased pHi markedly. We conclude that in GH4C1 cells, increasing [Ca2+]i reduces basal pHi through a mechanism dependent on influx of extracellular Ca2+ and independent of Na+/H+ exchange. In addition, elevation of [Ca2+]i and activation of protein kinase C act synergistically to enhance Na+/H+ exchange and increase pHi in acidified cells. Finally, normal cellular ATP is necessary for the activation of Na+/H+ exchange.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Törnquist K,Tashjian AH Jrdoi
10.1210/endo.130.2.1733720subject
Has Abstractpub_date
1992-02-01 00:00:00pages
717-25issue
2eissn
0013-7227issn
1945-7170journal_volume
130pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The antitumor properties of antibodies to the beta-subunit of hCG (hCG beta) coupled to tetanus toxoid (tt) were studied in hamsters implanted with the BeWo strain of human choriocarcinoma. After active immunization with hCG beta-tt mixed in complete Freund's adjuvant, hamsters synthesized large quantities of antibodi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-1-150
更新日期:1980-01-01 00:00:00
abstract::The monocarboxylate transporter Mct10 (Slc16a10; T-type amino acid transporter) facilitates the cellular transport of thyroid hormone (TH) and shows an overlapping expression with the well-established TH transporter Mct8. Because Mct8 deficiency is associated with distinct tissue-specific alterations in TH transport a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1800
更新日期:2014-01-01 00:00:00
abstract::Intracellular cargo transport relies on microtubules and motor proteins such as kinesins and dyneins. Currently we have ample knowledge of the mechanisms by which motor proteins propel themselves along the microtubules, but little is known about intracellular factors that regulate motor speed. Here we show that proadr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1685
更新日期:2012-04-01 00:00:00
abstract::Adult Leydig cells are derived from proliferating stem/progenitor Leydig cells in the infant testis and subsequent differentiation to steroidogenic cells in adult mice. Leydig cell proliferation in the infant testis occurs primarily in response to increased levels of LH that induce Leydig cell expression of neuregulin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1478
更新日期:2016-12-01 00:00:00
abstract::The effects of L-arginine and its derivatives NG-methyl-L-arginine (Meth-arg) and NG-nitro-L-arginine (Nit-arg) on the insulin release and glucose oxidation of isolated rat islets were investigated to evaluate possible influences of nitric oxide. Also, the insulin release and flow distribution within the perfused rat ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-6-3081
更新日期:1991-06-01 00:00:00
abstract::The present study employed the dehydroepiandrosterone (DHA) loading test to determine if the placenta in baboons remains functional following fetectomy. A bolus iv injection of 100 mg DHA was given to 4 baboons prior to and at various intervals following fetectomy at midgestation. Blood samples were withdrawn over a 4...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-2-843
更新日期:1985-02-01 00:00:00
abstract::There is evidence that the IGF system plays an important role in the growth and function of the thyroid gland. Proteolysis is an important posttranslational process that modulates the affinity of IGF binding proteins (IGFBPs) to IGFs, thus regulating their activity. IGFBP-3 has been shown to be cleaved by members of t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1378
更新日期:2004-03-01 00:00:00
abstract::To evaluate the relative contribution of the GH/IGF axis to the development of peak bone mineral density (BMD), we measured skeletal changes in IGF-I knockout (KO), IGF-II KO, and GH-deficient lit/lit mice and their corresponding control mice at d 23 (prepubertal), 31 (pubertal), and 56 (postpubertal) in the entire fe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220948
更新日期:2003-03-01 00:00:00
abstract::Insulin and protein kinase B (or Akt) play critical roles in cardiomyocytic growth and survival. High concentrations of glucocorticoids antagonize insulin's action. To examine whether endogenous glucocorticoids modulate insulin's effect on Akt signaling in the protein and glycogen synthetic pathways in myocardium, we ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1429
更新日期:2004-03-01 00:00:00
abstract::Circulating angiotensin II (ANGII) elicits water intake and activates the hypothalamic-pituitary-adrenal (HPA) axis by stimulating angiotensin type 1 receptors (AT1Rs) within circumventricular organs. The subfornical organ (SFO) and the organum vasculosum of the lamina terminalis (OVLT) are circumventricular organs th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0477
更新日期:2008-12-01 00:00:00
abstract::The extensive colocalization of insulin receptor (IR) and insulin-like growth factor-I receptor (IGFR) messenger RNAs during central nervous system development, together with the effects of insulin and IGF-I in neurogenesis, raises the question of how stage- and factor-specific signaling occurs. Thus, it is necessary ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.1.6393
更新日期:1999-01-01 00:00:00
abstract::Prolonged exposure to elevated FFA levels has been shown to induce peripheral insulin resistance and to alter the beta-cell secretory response to glucose. To investigate the effects of FFAs on preproinsulin gene expression, we measured insulin release, cell content, and messenger RNA (mRNA) levels in rat islets after ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.9.6953
更新日期:1999-09-01 00:00:00
abstract::Invasion of human trophoblasts is promoted through activation of wingless (Wnt) signaling, suggesting a role of the pathway in placental development and morphogenesis. However, details on the process such as involvement of canonical and/or noncanonical Wnt signaling cascades as well as their target genes are largely u...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0557
更新日期:2010-01-01 00:00:00
abstract::The aim of this study was to determine whether bisphenol A (BPA) has adverse effects on cardiovascular functions in CD-1 mice and define sex-specific modes of BPA action in the heart. Dams and analyzed progeny were maintained on a defined diet containing BPA (0.03, 0.3, 3, 30, or 300 ppm) that resulted in BPA exposure...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1847
更新日期:2015-03-01 00:00:00
abstract::Analysis of GH gene structure in the mouse permits evolutionary comparisons with GH gene structure in the rat and provides information about the mechanism responsible for heritable deficiencies of anterior pituitary hormones. The little mutation in mice is analogous to autosomal recessive, isolated, partial deficiency...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-5-1672
更新日期:1982-05-01 00:00:00
abstract::GH receptor (GHR) mediates the anabolic and metabolic effects of GH. We previously characterized a monoclonal antibody (anti-GHR(ext-mAb)) that reacts with subdomain 2 of the rabbit GHR extracellular domain (ECD) and is a conformation-specific inhibitor of GH signaling in cells bearing rabbit or human GHR. Notably, th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1336
更新日期:2011-12-01 00:00:00
abstract::Serum LH and FSH were measured at 60, 30, and 0 min before, at 5, 15, and 30 min during, and at 10, 45, and 90 min after bilateral electrical stimulation (ES) of various hypothalamic regions in 12 unanesthetized ovariectomized rhesus monkeys. ES of the arcuate-ventromedial nuclei (medial basal hypothalamus; MBH) induc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-2-314
更新日期:1977-02-01 00:00:00
abstract::Actions of protein products resulting from alternative splicing of the Igf1 gene have received increasing attention in recent years. However, the significance and functional relevance of these observations remain poorly defined. To address functions of IGF-I splice variants, we examined the impact of loss of IGF-IEa a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1279
更新日期:2011-05-01 00:00:00
abstract::Antisera against proopiomelanocortin (POMC)-derived peptides have previously been employed to demonstrate immunostainable materials in the male reproductive tract and in the corpus luteum of rat ovary. The present study was designed to determine how the distribution of such stainable materials varies in mouse ovary as...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-1-378
更新日期:1984-07-01 00:00:00
abstract::There is growing international interest in how environmental conditions experienced during development can shape adult phenotypes and the extent to which such induced changes are adaptive. One physiological system that links an individual to changes in environmental circumstances during development is the hypothalamic...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1471
更新日期:2009-04-01 00:00:00
abstract::In this study we tested the hypothesis that receptor-mediated transport of urocortin across the blood-brain barrier (BBB) undergoes developmental changes. Urocortin is a peptide produced by both selective brain regions and peripheral organs, and it is involved in feeding, memory, mood, cardiovascular functions, and im...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1039
更新日期:2010-03-01 00:00:00
abstract::A soluble form of the amino-terminal extracellular (ecto-) domain of the human TSH receptor was generated. This protein was capable of binding TSH and autoimmune antibodies found in Graves' patients. A deletion mutant of the ectodomain lacking nine amino acids in the C-terminal region lost its ability to interact with...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.2.5748
更新日期:1998-02-01 00:00:00
abstract::Notch signaling is an evolutionarily conserved pathway, which regulates cell proliferation, differentiation, and apoptosis. It has been reported that the members of Notch signaling are expressed in mammalian ovaries, but the exact functions of this pathway in follicle development is still unclear. In this study, prima...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1182
更新日期:2011-06-01 00:00:00
abstract::A method to prepare suspension cultures of highly viable pinealocytes is described. Pineal glands of neonatal or adult rats can be used; the best yield is obtained from neonatal glands. Morphological examination of the cells indicates that they have a normal, healthy appearance and retain some structures typical of ne...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-4-1483
更新日期:1978-10-01 00:00:00
abstract::We have previously reported that neurotensin (NT) is released from the small intestine and elevated in the hepatic-portal circulation in response to the perfusion of the small intestine with a micellar solution of oleic acid. In order to determine the minimum acyl chain length and whether the presence of a carboxylic ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-3-1133
更新日期:1985-03-01 00:00:00
abstract::The Pax6 transcription factor is crucial for pancreatic α-cells. Indeed, Pax6-deficient mouse models are characterized by markedly altered α-cell differentiation. Our objective was to investigate the role of Pax6 in glucagon secretion process. We used a Pax6-deficient model in rat primary enriched-α cells with specifi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1425
更新日期:2012-09-01 00:00:00
abstract::Tuberoinfundibular peptide is a recently discovered agonist for the PTH receptor-2; the latter has a wide distribution including the external zone of the median eminence of the hypothalamus, suggesting a role in neuroendocrine function. We have investigated the effects of tuberoinfundibular peptide on the hypothalamo-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.8.8308
更新日期:2001-08-01 00:00:00
abstract::The perinatal rat exhibits low serum TSH levels in the presence of very low concentrations of T3 and T4. We had reported previously that this animal does not require endogenous TRH for TSH release. We investigated here whether the inhibitory hormone somatostatin (SS) has a role in maintaining the low TSH secretory set...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1683
更新日期:1985-10-01 00:00:00
abstract::In rat pancreatic islets, the effect of old age (24-month-old) on [125I]insulin binding, glucose-induced insulin release and inhibition of insulin secretion by exogenous insulin were studied. The results were compared with corresponding data obtained from young (3-month-old) rats. Specifically bound [125I]insulin in i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-6-2147
更新日期:1983-06-01 00:00:00
abstract::Bone marrow-derived cells are major cellular components of human decidua, with macrophages comprising about 30% of the cells in term tissue. Because cytokines released by bone marrow-derived cells are known to affect hormone release in many tissues, we examined whether cytokines affect the release of PRL from human de...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.1.8275950
更新日期:1994-01-01 00:00:00