The dual actions of angiogenesis and anti-apoptosis induced by an isolated fraction from Geum japonicum repair muscle ischemia.

abstract：:Treatment of human pulmonary artery smooth muscle cells (HPASMCs) with the thromboxane A2 receptor antagonist, SQ29548 inhibited U46619 stimulation of phospholipase D (PLD) and NADPH oxidase activities in the cell membrane. Pretreatment with apocynin inhibited U46619 induced increase in NADPH oxidase activity. The cel...

journal_title：Archives of biochemistry and biophysics

authors： Chakraborti S,Sarkar J,Chowdhury A,Chakraborti T

更新日期：2017-11-01 00:00:00

abstract：:There is a current interest in dietary compounds (such as trans-resveratrol) that can inhibit or reverse oxidative stress, the common pathway for a variety of brain disorders, including Alzheimer's disease and stroke. The objective of the present study was to investigate the effects of resveratrol, under conditions of...

journal_title：Archives of biochemistry and biophysics

authors： de Almeida LM,Leite MC,Thomazi AP,Battu C,Nardin P,Tortorelli LS,Zanotto C,Posser T,Wofchuk ST,Leal RB,Gonçalves CA,Gottfried C

更新日期：2008-12-01 00:00:00

abstract：:A phospho-null Ala substitution at protein kinase C (PKC)-targeted cardiac troponin I (cTnI) S43/45 reduces myocyte and cardiac contractile function. The goal of the current study was to test whether cTnIS43/45N is an alternative, functionally conservative substitution in cardiac myocytes. Partial and more extensive e...

journal_title：Archives of biochemistry and biophysics

authors： Lang SE,Stevenson TK,Xu D,O'Connell R,Westfall MV

更新日期：2016-07-01 00:00:00

abstract：:The effects of extracellular ATP on phosphoinositide metabolism and intracellular Ca2+ homeostasis were studied in Ehrlich ascites tumor cells. Cytosolic [Ca2+] was measured using either quin 2 or the recently described indicator fura 2. Addition of 0.5-25 microM extracellular ATP to intact cells results in a rapid mo...

journal_title：Archives of biochemistry and biophysics

authors： Dubyak GR

更新日期：1986-02-15 00:00:00

abstract：:Calmodulin, a calcium-binding protein with no known enzymatic activity but multiple, in vitro effector activities, has been purified to apparent homogeneity from the unicellular green alga Chlamydomonas reinhardtii and compared to calmodulin from vertebrates and higher plants. Chlamydomonas calmodulin was characterize...

journal_title：Archives of biochemistry and biophysics

authors： Schleicher M,Lukas TJ,Watterson DM

更新日期：1984-02-15 00:00:00

abstract：:The severe acute respiratory syndrome coronavirus papain-like protease (SARS-CoV PLpro) carries out N-terminal processing of the viral replicase polyprotein, and also exhibits Lys48-linked polyubiquitin chain debranching and ISG15 precursor processing activities in vitro. Here, we used SDS-PAGE and fluorescence-based ...

journal_title：Archives of biochemistry and biophysics

authors： Lindner HA,Lytvyn V,Qi H,Lachance P,Ziomek E,Ménard R

更新日期：2007-10-01 00:00:00

abstract：:A green manganese desferrioxamine complex is rapidly formed at room temperature upon stirring freshly precipitated manganese dioxide in a solution of the ligand. Spectral studies and low-temperature ESR indicate that this compound, which has been previously described as a manganese(III) complex, is better characterize...

journal_title：Archives of biochemistry and biophysics

authors： Rush JD,Maskos Z,Koppenol WH

更新日期：1991-08-15 00:00:00

abstract：:The common notion in the protein world holds that proteins are synthesized as a linear polypeptide chain, followed by folding into a unique, functional 3D-structure. As outlined in many articles of this volume, this is in fact the case for a great proportion of the proteome. Many proteins and protein domains, however,...

journal_title：Archives of biochemistry and biophysics

authors： Kovacs D,Szabo B,Pancsa R,Tompa P

更新日期：2013-03-01 00:00:00

abstract：:Cytochrome P450 (P450) 2D6 is the classic human liver debrisoquine 4-hydroxylase, the first human P450 for which genetic polymorphism was clearly demonstrated. We prepared 11 different constructs of P450 2D6, with modification at the N-terminus, for expression in Escherichia coli with the vector pCW. These varied cons...

journal_title：Archives of biochemistry and biophysics

authors： Gillam EM,Guo Z,Martin MV,Jenkins CM,Guengerich FP

更新日期：1995-06-01 00:00:00

abstract：:An obligatory step in retroviral growth is the integration of a DNA copy of the viral RNA into the genomic DNA of the host. Recombinant human immunodeficiency virus type I (HIV-1) integrase (IN) expressed in Escherichia coli efficiently catalyzes the overall in vitro integration reaction, namely the processing of the ...

journal_title：Archives of biochemistry and biophysics

authors： Carteau S,Mouscadet JF,Goulaouic H,Subra F,Auclair C

更新日期：1993-09-01 00:00:00

abstract：:A chromatographic adsorbent (TED-Sepharose) capable of chelating various di- and trivalent metal ions was prepared by introducing tris(carboxymethyl)ethylenediamine into agarose. This gel was charged with Al3+, Ga3+, In3+, and Tl3+ to form immobilized-metal affinity adsorbents, and their adsorption behavior toward ser...

journal_title：Archives of biochemistry and biophysics

authors： Porath J,Olin B,Granstrand B

更新日期：1983-09-01 00:00:00

abstract：:A full-length recombinant mutant of diphtheria toxin containing serine in place of a crucial active-site glutamate has been purified and characterized. The serine substitution caused a minor structural alteration in the toxin as measured by trypsinolysis. ADP-ribosyltransferase activity and cytotoxicity of the mutant ...

journal_title：Archives of biochemistry and biophysics

authors： O'Keefe DO

更新日期：1992-08-01 00:00:00

abstract：:Substrate specificity of human mitochondrial low Km aldehyde dehydrogenase (EC 1.2.1.3) E2 isozyme has been investigated employing p-nitrophenyl esters of acyl groups of two to six carbon atoms and comparing with that of aldehydes of one to eight carbon atoms. The esterase reaction was studied under three conditions: ...

journal_title：Archives of biochemistry and biophysics

authors： Mukerjee N,Pietruszko R

更新日期：1992-11-15 00:00:00

abstract：:Linamarase (EC 3.2.1.21) was purified from cassava petiole, stem, and root cortex by ammonium sulfate precipitation, column chromatography on Sepharose 6B, and chromatofocusing. The last step resolved the enzyme from each source into three forms with pI values of 4.3, 3.3, and 2.9. Each form was found to be oligomeric...

journal_title：Archives of biochemistry and biophysics

authors： Eksittikul T,Chulavatnatol M

更新日期：1988-10-01 00:00:00

abstract：:In this study, we have analyzed interflavin electron transfer reactions from FAD to FMN in both the full-length inducible nitric oxide synthase (iNOS) and its reductase domain. Comparison is made with the interflavin electron transfer in NADPH-cytochrome P450 reductase (CPR). For the analysis of interflavin electron t...

journal_title：Archives of biochemistry and biophysics

authors： Yamamoto K,Kimura S,Shiro Y,Iyanagi T

更新日期：2005-08-01 00:00:00

abstract：:The most common form of muscular dystrophy is known as Myotonic dystrophy Type 1 (DM1) in adults. It was aimed to investigate the relationship between antioxidant imbalance and diaphragm thickness with pulmonary function test results in peripheral blood of Myotonic Dystrophy Type 1 patients. In the prospective study, ...

journal_title：Archives of biochemistry and biophysics

authors： Koc F,Atli G,Menziletoglu SY,Kose S

更新日期：2020-02-15 00:00:00

abstract：:There is evidence to suggest that extracellular matrix molecules, such as proteoglycans, are involved in the regulation of mineral deposition in calcifying tissues. One mineralizing system which is characterized by extremely rapid mineralization is the hen eggshell. This eggshell consists of a pair of nonmineralized e...

journal_title：Archives of biochemistry and biophysics

authors： Arias JL,Carrino DA,Fernández MS,Rodríguez JP,Dennis JE,Caplan AI

更新日期：1992-10-01 00:00:00

abstract：:The nuclear Ah receptor from mouse hepatoma (Hepa-1c1c9) cells is a 176-kDa multimeric protein which is stable under conditions of up to 1 M KCl. Under denaturing conditions, the Hepa-1 nuclear receptor can be dissociated into a ligand-binding subunit of Mr approximately 91,000. The identity of subunits that compose t...

journal_title：Archives of biochemistry and biophysics

authors： Prokipcak RD,Denison MS,Okey AB

更新日期：1990-12-01 00:00:00

abstract：:A novel serum protein inhibiting specifically the enzymatic activity of the basic phospholipase A(2) (PLA(2)) from the venom of the Chinese mamushi snake (Agkistrodon blomhoffii siniticus) was purified from a nonvenomous Colubridae snake, Elaphe quadrivirgata. The purified inhibitor was a 150-kDa glycoprotein having a...

journal_title：Archives of biochemistry and biophysics

authors： Okumura K,Inoue S,Ikeda K,Hayashi K

更新日期：2002-12-01 00:00:00

abstract：:The activity of thymidylate synthetase in the liver of the ddY strain male mouse increased transitorily according to the increase in tumor cell number at maximum 7-9 days after ip transplantation of Ehrlich ascites tumor. The enzyme was able to be purified from the tumor host mouse liver or from the normal mouse liver...

journal_title：Archives of biochemistry and biophysics

authors： Asanagi M,Moriyama Y,Fujimura S

更新日期：1988-12-01 00:00:00

abstract：:Oxygen free radicals and hydroperoxides have been postulated to play a causal role in the aging process, implying that antioxidant enzymes may act as longevity determinants. Catalase (H2O2:H2O2 oxidoreductase; EC1.11.1.6) is the sole enzyme involved in the elimination of H2O2 in Drosophila melanogaster; glutathione pe...

journal_title：Archives of biochemistry and biophysics

authors： Orr WC,Sohal RS

更新日期：1992-08-15 00:00:00

abstract：:The three-dimensional conformation of a protein provides a wealth of biochemical information and with the advent of cloning techniques that allow the preparation of proteins almost at will, a renewed interest has arisen in the crystallographic determination of protein structures. As in any research technique, however,...

journal_title：Archives of biochemistry and biophysics

authors： Holden HM,Rayment I

更新日期：1991-11-15 00:00:00

abstract：:The cardiac contraction-relaxation cycle is controlled by a sophisticated set of machinery. Of particular interest, is the revelation that allosteric networks transmit effects of binding at one site to influence troponin complex dynamics and structural-mediated signaling in often distal, functional sites in the myofil...

journal_title：Archives of biochemistry and biophysics

authors： Marques MA,Parvatiyar MS,Yang W,de Oliveira GAP,Pinto JR

更新日期：2019-03-15 00:00:00

abstract：:Osmolytes are small organic molecules accumulated by organisms under stress conditions to protect macromolecular structure and function. In the present study, we have investigated the effect of several binary osmolyte mixtures on the protein folding/stability and function of RNase-A. For this, we have measured ΔGD(o) ...

journal_title：Archives of biochemistry and biophysics

authors： Warepam M,Singh LR

更新日期：2015-05-01 00:00:00

abstract：:Several plasma membrane phospholipids have been studied for their ability to modulate the activity of the plasma membrane H(+)-ATPase of Saccharomyces cerevisiae. We show here that phosphatidylinositol phosphate (PIP), phosphatidylinositol bisphosphate (PIP2), and/or the phosphatidylinositol and PIP kinases are locali...

journal_title：Archives of biochemistry and biophysics

authors： Patton JL,Lester RL

更新日期：1992-01-01 00:00:00

abstract：:Human CXCR4 was expressed in Sf9 insect cells using the Bac-to-Bac baculovirus expression system. The recombinant receptor exhibited ligand binding activities with a K(d) value (3.3 nM) comparable to that of the native receptor. The role of four conserved cysteinyl residues was explored by site-directed mutagenesis. E...

journal_title：Archives of biochemistry and biophysics

authors： Zhou H,Tai HH

更新日期：2000-01-01 00:00:00

abstract：:Several 13C isotope effects of relevance to reactions involving carbamate and carbamoyl phosphate have been determined. The fractionation factor of carbamate relative to aqueous CO2 is 1.011; the equilibrium isotope effect on the reaction catalyzed by carbamate kinase is 0.9983. From these data we can calculate that t...

journal_title：Archives of biochemistry and biophysics

authors： Tipton PA,Cleland WW

更新日期：1988-01-01 00:00:00

abstract：:The relationship between ligand-protein affinity and the extent of protein stabilization induced by such interactions has been investigated using the binding of polyanions to acidic fibroblast growth factor (aFGF) as a model system. It was found that the experimentally observed unfolding rate constant of aFGF consists...

journal_title：Archives of biochemistry and biophysics

authors： Mach H,Middaugh CR

更新日期：1994-02-15 00:00:00

abstract：:Phenylalanine ammonia-lyase forms trans-cinnamate from L-phenylalanine, and thus stands at a gateway to secondary metabolism in higher plants. L-alpha-Amino-oxy-beta-phenylpropanoic acid (L-AOPP), a very effective competitive inhibitor of this enzyme, is most probably a transition-state analog for the elimination reac...

journal_title：Archives of biochemistry and biophysics

authors： Jones DH,Northcote DH

更新日期：1984-11-15 00:00:00

abstract：:The single-stranded, DNA-dependent ATPase activity of purified recA protein was found to be inhibited competitively by ribose-modified analogs of ATP, 3'-O-anthraniloyl-ATP (Ant-ATP), and 3'-O-(N-methylanthraniloyl)-ATP (Mant-ATP). The Ki values for Ant-ATP and Mant-ATP were around 7 and 3 microM at pH 7.5, respective...

journal_title：Archives of biochemistry and biophysics

authors： Karasaki Y,Higashi K

更新日期：1984-09-01 00:00:00