The effects of catalase gene overexpression on life span and resistance to oxidative stress in transgenic Drosophila melanogaster.

abstract：:Insulin has pleiotropic effects on sensitive cells, including the regulation of specific mRNA accumulation initiated by the binding of insulin to its plasma membrane receptor. Lectins, such as wheat germ agglutinin (WGA) and concanavalin A (Con A), are known to be insulin mimetic. It is thought that WGA and Con A inte...

journal_title：Archives of biochemistry and biophysics

authors： Messina JL,Hamlin J,Larner J

更新日期：1987-04-01 00:00:00

abstract：:Lysosomes were isolated from Chinese hamster ovary cells by fractionation of a postnuclear supernatant in consecutive density gradients. By marker enzyme analysis, the preparation was 63-fold enriched for lysosomes compared to the homogenate and contained at most trace amounts of marker activities for plasma membrane,...

journal_title：Archives of biochemistry and biophysics

authors： Madden EA,Wirt JB,Storrie B

更新日期：1987-08-15 00:00:00

abstract：:Mitochondrial carbonic anhydrase has previously been quantitated in liver mitochondria; it was not detected in guinea pig kidney cortical mitochondria. Evidence of this enzyme in rat kidney cortical mitochondria is reported. Electron microscopy showed that intact mitochondria were free of other intracellular organelle...

journal_title：Archives of biochemistry and biophysics

authors： Dodgson SJ,Contino LC

更新日期：1988-01-01 00:00:00

abstract：:Free and endoplasmic reticulum-bound polyribosomes from rat heart were examined for their ferritin mRNA content. A procedure for separation and purification of the two ribosome populations that produced good yields of homogeneous mono- and polyribosomes with no contaminating ultrastructures and gave distinctive sedime...

journal_title：Archives of biochemistry and biophysics

authors： Campbell CH,Ismail R,Linder MC

更新日期：1989-08-15 00:00:00

abstract：:A congenital diaphragmatic hernia (CDH) is an anomaly caused by defects in the diaphragm; the resulting limited thorax cavity in turn restricts lung growth (pulmonary hypoplasia). This condition is related to pulmonary hypertension. Despite advances in neonatal CDH therapy, the mortality for severe pulmonary hypoplasi...

journal_title：Archives of biochemistry and biophysics

authors： Ito Y,Tsuda H,Imai K,Miki R,Miura M,Tachi A,Tano S,Hirako-Takamura S,Moriyama Y,Ushida T,Kobayashi T,Sumigama S,Kajiyama H,Kikkawa F,Kotani T

更新日期：2021-01-20 00:00:00

abstract：:Conversion of a steroid receptor complex from the 8 S to the 4S form results in new interactions between the steroid and the receptor and/or formation of new intra-protein bonds within the receptor molecule itself. These bonds must be broken before the steroid is released. In order to localize these newly formed inter...

journal_title：Archives of biochemistry and biophysics

authors： Wolfson AJ,Ryu ML,Baghdady R

更新日期：1991-02-01 00:00:00

abstract：:One- and two-dimensional 1H NMR experiments were used to determine the chemical shifts of resonances arising from 29 residues of RNase A in H2O at 17 pH values ranging from 1.2 to 7.9 Nearly all resonances displaying pH-induced changes in chemical shift greater than 0.1 ppm were monitored. Individual plots of the chem...

journal_title：Archives of biochemistry and biophysics

authors： Baker WR,Kintanar A

更新日期：1996-03-01 00:00:00

abstract：:Dark incubation of envelope vesicles derived from a strain of Halobacterium halobium that lacks bacteriorhodopsin but contains halorhodopsin and a third rhodopsin-like pigment caused a decrease in the flash yield [the amplitude of a transient absorbance change of flash reactive component(s) by flash] of halorhodopsin ...

journal_title：Archives of biochemistry and biophysics

authors： Kamo N,Hazemoto N,Kobatake Y,Mukohata Y

更新日期：1985-04-01 00:00:00

abstract：:The effect of ligating the alpha2-macroglobulin signaling receptor (alpha2MSR) with receptor-recognized forms of alpha2M (alpha2M*) was studied with respect to phospholipase D (PLD) activity in murine macrophages, their plasma membranes, and nuclei. PLD activity in plasma membranes and nuclei increased linearly up to ...

journal_title：Archives of biochemistry and biophysics

authors： Misra UK,Pizzo SV

更新日期：1999-03-01 00:00:00

abstract：:The susceptibility of 40S and 60S ribosomal subunits from Saccharomyces cerevisiae to digestion with varying concentrations of trypsin was studied by two-dimensional electrophoresis and quantitative measurements of the protein remaining with the ribosomal particles after trypsin treatment. Proteins from both subunits ...

journal_title：Archives of biochemistry and biophysics

authors： Lee JC,Haley WT Jr

更新日期：1983-07-01 00:00:00

abstract：:The enzyme catalase catalyzes the breakdown of hydrogen peroxide into oxygen and water. It is the main regulator of hydrogen peroxide metabolism. Hydrogen peroxide is a highly reactive small molecule formed as a natural byproducts of energy metabolism. Excessive concentrations may cause significant damages to protein,...

journal_title：Archives of biochemistry and biophysics

authors： Góth L,Nagy T

更新日期：2012-09-15 00:00:00

abstract：:Two enzymatically modified derivatives of tobramycin have been prepared by gentamicin nucleotidyl transferase-catalyzed adenylylation of tobramycin, using ATP and (Sp)-ATP alpha S as adenylylation substrates. (EC 2.7.7.46). The 1H, 13C, and 31P NMR spectra have been assigned for tobramycin, 2''-(adenosine-5'-phosphory...

journal_title：Archives of biochemistry and biophysics

authors： Van Pelt JE,Mooberry ES,Frey PA

更新日期：1990-08-01 00:00:00

abstract：:Recombinant glutathione S-transferase P (GST-P) was purified in a homogeneous state. Fatty acid analysis of the enzyme revealed that the final enzyme preparation endogenously bound fatty acids, mostly palmitic acid or stearic acid, which were difficult to dissociate from the complex. Temperature-dependent analysis by ...

journal_title：Archives of biochemistry and biophysics

authors： Nishihira J,Ishibashi T,Sakai M,Tsuda S,Hikichi K

更新日期：1993-04-01 00:00:00

abstract：:Mice were supplemented with low and high doses of natural and synthetic vitamin E, T cells from the spleen isolated and stimulated with plate-bound anti-CD3 and soluble anti-CD28, and gene expression changes assessed by gene array experiments. The data obtained indicate significant qualitative and quantitative differe...

journal_title：Archives of biochemistry and biophysics

authors： Han SN,Pang E,Zingg JM,Meydani SN,Meydani M,Azzi A

更新日期：2010-03-01 00:00:00

abstract：:Human soluble epoxide hydrolase (hsEH) has been shown to play a role in regulating blood pressure and inflammation. HsEH consists of an N-terminal phosphatase and a C-terminal epoxide hydrolase domain. In the present study, we examined the effects of polymorphisms in the hsEH gene on phosphatase activity, enzyme stabi...

journal_title：Archives of biochemistry and biophysics

authors： Srivastava PK,Sharma VK,Kalonia DS,Grant DF

更新日期：2004-07-15 00:00:00

abstract：:Histone-DNA templates for bacteriophage T7 RNA polymerase were assembled from a plasmid containing a promoter and a terminator for this polymerase, (H3 x H4)2 tetramers deprived of their tail domains, and H2A x H2B dimers. Histone (H3 x H4)2 tetramers lacking their terminal domains were obtained from trypsin-digested ...

journal_title：Archives of biochemistry and biophysics

authors： Hernández F,López-Alarcón L,Puerta C,Palacián E

更新日期：1998-10-01 00:00:00

abstract：:Phorbol esters induce rapid downregulation of PAF receptors on human neutrophils. The molecular mechanism responsible for this observation has not been elucidated. In the present study, treatment of neutrophils with PMA (10(-7) M) for 10 min caused a complete loss of PAF receptors from the cell surface as determined b...

journal_title：Archives of biochemistry and biophysics

authors： Zhou W,Olson MS

更新日期：1994-08-15 00:00:00

abstract：:Dideoxyosones (DDOs) are intermediates in the synthesis of advanced glycation endproducts (AGEs), such as pentosidine and glucosepane. Although the formation of pentosidine and glucosepane in the human lens has been firmly established, the formation of DDOs has not been demonstrated. The aim of this study was to devel...

journal_title：Archives of biochemistry and biophysics

authors： Linetsky M,Kaid Johar SR,Meltretter J,Padmanabha S,Parmar T,Vasavada AR,Pischetsrieder M,Nagaraj RH

更新日期：2011-10-01 00:00:00

abstract：:The single cysteine residue of human serum albumin (HSA-SH) is the most abundant plasma thiol. HSA transports fatty acids (FA), a cargo that increases under conditions of diabetes, exercise or adrenergic stimulation. The stearic acid-HSA (5/1) complex reacted sixfold faster than FA-free HSA at pH 7.4 with the disulfid...

journal_title：Archives of biochemistry and biophysics

authors： Torres MJ,Turell L,Botti H,Antmann L,Carballal S,Ferrer-Sueta G,Radi R,Alvarez B

更新日期：2012-05-01 00:00:00

abstract：:Glycosphingolipids were extracted from human semen and purified. Based on the fluorometric assay of sphingosine, in spermatozoa a content of 4.4 +/- 0.9 nmol/10(8) cells of gangliosides and 22.1 +/- 1.7 nmol/10(8) cells of neutral glycosphingolipids was determined. Seminal plasma contained 4.1 +/- 0.6 nmol ganglioside...

journal_title：Archives of biochemistry and biophysics

authors： Ritter G,Krause W,Geyer R,Stirm S,Wiegandt H

更新日期：1987-09-01 00:00:00

abstract：:The lysosomal cysteine proteinases, cathepsins B, H, and L, were all shown to bind to alpha 2-macroglobulin. The bound enzymes remained active against low-molecular-weight synthetic substrates and bound the active-site-directed inhibitor, benzyloxycarbonyl-125I-Tyr-Ala-diazomethane. Binding of the radiolabeled inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Mason RW

更新日期：1989-09-01 00:00:00

abstract：:A type II ribosome-inactivating protein (RIP) was isolated from the bark tissue of Japanese elderberry (Sambucus sieboldiana) and named sieboldin-b. Sieboldin-b is a heterodimeric protein consisting of 27- and 33-kDa subunits and showed strong ribosome-inactivating activity in vitro but did not show in vivo toxicity. ...

journal_title：Archives of biochemistry and biophysics

authors： Rojo MA,Yato M,Ishii-Minami N,Minami E,Kaku H,Citores L,Girbés T,Shibuya N

更新日期：1997-04-15 00:00:00

abstract：:The properties of porcine platelet acyltransferases which catalyze the incorporation of unsaturated fatty acids into the 2 positions of phospholipids were compared with those of porcine liver microsomes and rat liver microsomes. There were significant differences in the relative rates of incorporation of acyl groups i...

journal_title：Archives of biochemistry and biophysics

authors： Inoue M,Murase S,Okuyama H

更新日期：1984-05-15 00:00:00

abstract：:The carbonyl cofactor of bovine plasma amine oxidase (EC 1.4.3.6), recently shown to be 6-hydroxydopa (also known as topa), has been spin labeled to the extent of one label per enzyme dimer molecule, using 4-amino-2,2,6,6-tetramethylpiperidine-N-oxyl (4-amino-TEMPO) and 4-hydrazino-TEMPO followed by reduction with bor...

journal_title：Archives of biochemistry and biophysics

authors： Greenaway FT,O'Gara CY,Marchena JM,Poku JW,Urtiaga JG,Zou Y

更新日期：1991-03-01 00:00:00

abstract：:Incubation of rat hepatocytes with 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), an activator of the 5'-AMP-activated protein kinase (AMPK), produced a twofold stimulation of palmitate oxidation and of the activity of carnitine palmitoyltransferase I (CPT-I), together with a profound decrease of the activity ...

journal_title：Archives of biochemistry and biophysics

authors： Velasco G,Geelen MJ,Guzmán M

更新日期：1997-01-15 00:00:00

abstract：:A 42-kDa plant outer mitochondrial membrane protein, MOM42, has been identified as an essential component of the plant mitochondrial precursor protein translocation apparatus. Immunological cross-reactivity has been detected between antibodies raised against both Neurospora and yeast mitochondrial outer membrane prote...

journal_title：Archives of biochemistry and biophysics

authors： Perryman RA,Mooney B,Harmey MA

更新日期：1995-02-01 00:00:00

abstract：:Two isoforms of regulatory (R) subunit of cAMP-dependent protein kinase (PKA), named R(myt1) and R(myt2), were identified so far in the sea mussel Mytilus galloprovincialis. Out of them, only R(myt2) was phosphorylated in vitro by casein kinase 2 (CK2) using GTP as phosphate donor. CK2 catalytic subunit (CK2alpha) its...

journal_title：Archives of biochemistry and biophysics

authors： Bardales JR,Hellman U,Villamarín JA

更新日期：2007-05-01 00:00:00

abstract：:A thymidine-diphospho-4-keto-6-deoxy-D-glucose epimerase was purified from Saccharopolyspora erythraea, the producer of the macrolide antibiotic erythromycin, by a high resolution chromatographic method that exploited the difference in behavior of the protein on ion exchange columns at pH 7.5 and 5.5. By this procedur...

journal_title：Archives of biochemistry and biophysics

authors： Jarvis BW,Hutchinson CR

更新日期：1994-01-01 00:00:00

abstract：:Hypertension is one of the major causes of mortality. Though a host of drugs are available for the treatment of hypertension, majority have been linked to adverse side effects, necessitating the need for research into natural compounds with fewer side effects. Kaempferol-7-O-α-L-rhamnopyroside (KR) is a glycosylated f...

journal_title：Archives of biochemistry and biophysics

authors： Tettey CO,Yang IJ,Shin HM

更新日期：2019-05-30 00:00:00

abstract：:The hnRNP A/B family contains abundant nuclear proteins with major roles in alternative splicing and the ability for nucleo-cytoplasmic shuttling. Compared to the best known members of this family (hnRNP A1, A2/B1), hnRNP A3 is a relatively less known protein. We report herein immunochemical studies with the hnRNP A3 ...

journal_title：Archives of biochemistry and biophysics

authors： Papadopoulou C,Boukakis G,Ganou V,Patrinou-Georgoula M,Guialis A

更新日期：2012-07-15 00:00:00