Abstract：
:Human p29 is a newly identified nuclear protein whose function is largely undetermined. We found that p29 associated with chromatin, interacted with MCM3, and localized with proliferating cell nuclear antigen foci in the S phase. Silencing of p29 using small interfering RNA duplexes reduced DNA synthesis and increased the expression of p107, a member of the RB family, and of cyclin-dependent kinase inhibitor p21, accompanied with a decreased expression of DNA polymerase alpha. Lethal events consisting of premature chromatin condensation with a reduced Chk1 phosphorylation were observed in p29-depleted cells in response to UV irradiation. Intriguingly, the phosphorylation of ataxia telangectasia-mutated kinases at S1981 was suppressed in p29-depleted HeLa cells with UV irradiation, but not in hydroxyurea- and ionizing radiation-treated cells. Taken together, these results reveal a novel function of p29 in the regulation of DNA replication checkpoint responses.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Chu PC,Yang YC,Lu YT,Chen HT,Yu LC,Chang MSdoi
10.1158/0008-5472.CAN-05-3229subject
Has Abstractpub_date
2006-09-01 00:00:00pages
8484-91issue
17eissn
0008-5472issn
1538-7445pii
66/17/8484journal_volume
66pub_type
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