Abstract：
:The abundance of circulating tumor cells (CTC) indicates patient prognosis. Molecular characterization of CTCs may add additional information about a patient's disease. However, currently available methods are limited by contamination with blood cells. We describe a study using a modified CTC-chip to capture CTCs from an orthotopic xenograft model. Using laser capture microscopy to collect CTCs from the chip, we compared transcripts from purified CTCs with those from primary and metastatic tissue. Transcriptional profiles showed strong concordance among primary, metastatic, and CTC sources. Moreover, cells captured on the chip were viable and could be expanded in culture. We conclude that the CTC-chip is a useful tool to further characterize animal models of cancer and that viable CTCs can be isolated and show transcriptional similarity to solid tumors.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Helzer KT,Barnes HE,Day L,Harvey J,Billings PR,Forsyth Adoi
10.1158/0008-5472.CAN-09-0801subject
Has Abstractpub_date
2009-10-01 00:00:00pages
7860-6issue
19eissn
0008-5472issn
1538-7445pii
0008-5472.CAN-09-0801journal_volume
69pub_type
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