Abstract:
:A simple technique is described for fixing colony-containing layers of soft agar and drying them onto microscopic slides. The method is extrapolated from techniques used in immunology for permanent preservation of immunodiffusion or immunoelectrophoresis plates. Slides prepared in this fashion are eminently suitable for subsequent analysis with a variety of techniques including conventional Papanicolaou or other staining methods as well as histochemistry, immunofluorescence, and autoradiography. In addition to research applications, the technique may have diagnostic applications and should greatly enhance both qualitative and quantitative analysis of the biology of hematopoietic and tumor colony formation.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Salmon SE,Buick RNsubject
Has Abstractpub_date
1979-03-01 00:00:00pages
1133-6issue
3eissn
0008-5472issn
1538-7445journal_volume
39pub_type
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