Abstract:
:To further understand the molecular mechanism by which 1,25(OH)2-vitamin D3 [1,25(OH2D3] rapidly stimulates intestinal calcium transport (termed "transcaltachia"), the effect of the calcium channel agonist BAY K8644 was studied in vascularly perfused duodenal loops from normal, vitamin D-replete chicks. BAY K8644, 2 mu M, was found to stimulate 45Ca2+ transport from the lumen to the vascular effluent to the same extent as physiological levels of 1,25(OH)2D3. The sterol and the Ca2+ channel agonist both increased 45Ca2+ transport 70% above control values within 2 min and 200% after 30 min of vascular perfusion. The effect of the Ca2+ channel agonist was dose dependent. Also, 1,25(OH)2D3-enhanced transcaltachia was abolished by the calcium channel blocker nifedipine. Collectively, these results suggest the involvement of 1,25(OH)2D3 in the activation of basal lateral membrane Ca2+ channels as an early effect in the transcaltachic response.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
de Boland AR,Nemere I,Norman AWdoi
10.1016/0006-291x(90)91933-jsubject
Has Abstractpub_date
1990-01-15 00:00:00pages
217-22issue
1eissn
0006-291Xissn
1090-2104pii
0006-291X(90)91933-Jjournal_volume
166pub_type
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