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Differences in the active site environment of Aspergillus ficuum phytases.

Abstract：

:While Aspergillus ficuum phytaseA (phyA) was rapidly inactivated by 1,2-cyclohexanedione and phenylglyoxal, both specific modifiers of arginine, phytaseB (phyB) showed a markedly different behavior. First, phyB was totally insensitive to 1,2-cyclohexanedione even in the presence of 0.2 M guanidinium hydrochloride; second, the enzyme showed a great deal of resistance to inactivation by phenylglyoxal. Taken together, these results indicate that the chemical environment of the active site of phyB is very different from that of the active site of phyA. Despite sequence similarities of the active site region in these two proteins, their differential behavior to arginine modifiers indicates that other parts of the protein play a role in the active site formation. We expected some differences in the structure since the proteins have dissimilar kinetic parameters and pH optima.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Ullah AH,Sethumadhavan K

doi

10.1006/bbrc.1998.8117

subject

Has Abstract

pub_date

1998-02-13 00:00:00

pages

458-62

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(98)98117-7

journal_volume

243

pub_type

杂志文章

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