Abstract:
:The bifunctional enzyme dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) catalyzes the dehydration of dehydroquinate to dehydroshikimate and the reduction of dehydroshikimate to shikimate in the shikimate pathway. We report the first crystal structure of Arabidopsis DHQ-SDH with shikimate bound at the SDH site and tartrate at the DHQ site. The interactions observed in the DHQ-tartrate complex reveal a conserved mode for substrate binding between the plant and microbial DHQ dehydratase family of enzymes. The SDH-shikimate complex provides the first direct evidence of the role of active site residues in the catalytic mechanism. Site-directed mutagenesis and mechanistic analysis revealed that Asp 423 and Lys 385 are key catalytic groups and Ser 336 is a key binding group. The arrangement of the two functional domains reveals that the control of metabolic flux through the shikimate pathway is achieved by increasing the effective concentration of dehydroshikimate through the proximity of the two sites.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Singh SA,Christendat Ddoi
10.1021/bi060366+subject
Has Abstractpub_date
2006-06-27 00:00:00pages
7787-96issue
25eissn
0006-2960issn
1520-4995journal_volume
45pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Herpesvirus saimiri encodes a tyrosine kinase interacting protein (Tip) that binds to T-cell-specific tyrosine kinase Lck via multiple sequence motifs and controls its activity. The regulation of Lck by Tip represents a key mechanism in the transformation of human T-lymphocytes during herpesviral infection. In this st...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0485068
更新日期:2004-11-30 00:00:00
abstract::Several different monoclonal antibodies (mAbs) have been actively developed in the field of Alzheimer's disease (AD) for basic science and clinical applications; however, the binding kinetics of many of the mAbs with the beta-amyloid peptides (Abeta) are poorly understood. A panel of mAbs with different Abeta recognit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900523q
更新日期:2009-11-03 00:00:00
abstract::To study the function of exon 21 of the insulin receptor, a mutant human insulin receptor lacking this domain was constructed. The mutant HIR delta E21 cDNA was transfected into Rat-1 fibroblasts and stable cell lines were selected. The HIR delta E21 receptors were expressed on the cell surface, and they bound insulin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00212a021
更新日期:1993-12-14 00:00:00
abstract::The nuclear tRNA 3' processing activity from wheat has been characterized and partially purified. Several characteristics of the wheat nuclear 3' processing enzyme now allow this activity to be distinguished from its mitochondrial counterpart. The nuclear enzyme is an endonuclease, which we termed nuclear RNase Z. The...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992253e
更新日期:2000-02-29 00:00:00
abstract::The pre-steady-state kinetics of the trichodiene synthase reaction were investigated by rapid chemical quench methods. The single-turnover rate was found to be 3.5-3.8 s-1, a rate 40 times faster than the steady-state catalytic rate (kcat = 0.09 s-1) for trichodiene synthase-catalyzed conversion of farnesyl diphosphat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi963018o
更新日期:1997-07-08 00:00:00
abstract::The crystal structure of a human carbonic anhydrase II (CAII) variant, cis-proline-202-->alanine (P202A), has been determined at 1.7-A resolution, indicating that the wild-type geometry, including the cis-peptidyl linkage, is retained upon substitution of proline by alanine. The CO2 hydrase activity and affinity for s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00092a003
更新日期:1993-10-19 00:00:00
abstract::The side chain of the antifungal polyketide ansatrienin A produced by Streptomyces collinus contains a cyclohexanecarboxylic acid (CHC) derived moiety. This CHC in the coenzyme A activated form (CHC-CoA) is derived from shikimic acid via a pathway in which the penultimate step is the isomerization of 2-cyclohexenylcar...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0005714
更新日期:2000-06-27 00:00:00
abstract::The surface immobilization methods that allowed single-molecule motility experiments with native kinesin have not worked with the ncd motor protein and other kinesin-related motors. To solve this problem, a surfactant (Pluronic F108) was chemically modified with the metal-chelating group nitrilotriacetic acid (NTA) to...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9829175
更新日期:1999-04-20 00:00:00
abstract::We report the use of electrospray ionization (ESI) mass spectrometry (MS) in conjunction with online rapid mixing to monitor the kinetics of acid-induced ferrihemoglobin denaturation. Under equilibrium conditions, the hemoglobin mass spectrum is dominated by the intact heterotetramer. Dimeric and monomeric species are...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048501a
更新日期:2004-11-23 00:00:00
abstract::(+)-Delta-cadinene synthase (DCS) from Gossypium arboreum (tree cotton) is a sesquiterpene cyclase that catalyzes the cyclization of farnesyl diphosphate in the first committed step of the biosynthesis of gossypol, a phytoalexin that defends the plant from bacterial and fungal pathogens. Here, we report the X-ray crys...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900483b
更新日期:2009-07-07 00:00:00
abstract::The circular dichroism (CD) of Pfl filamentous virus has been examined over the temperature range 0-40 degrees C, in the absence and presence of Hg(II), Ag(I), and Cu(II). Thermal difference CD spectra were obtained by subtraction of spectra recorded above and below a thermally induced structure transition near 12 deg...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00410a010
更新日期:1988-05-17 00:00:00
abstract::P-glycoprotein (P-gp) is the most well-known ATP-binding cassette (ABC) transporter involved in unidirectional substrate translocation across the membrane lipid bilayer, thereby causing the typical multidrug resistance (MDR) phenotype expressed in many cancers. We observed that in human CEM acute lymphoblastic leukemi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048669w
更新日期:2005-03-22 00:00:00
abstract::The interaction of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, 4,5-dideoxyshikimate 3-phosphate (ddS3P), and [2-13C]-and [3-13C]phosphoenolpyruvate (PEP) has been examined by 13C NMR spectroscopy. Although no resonances due to a dead-end intermediate complex could be detected, an enzyme active site specific fo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00446a003
更新日期:1989-10-03 00:00:00
abstract::The effect of both antimitotic drugs and nucleotide analogues on the magnesium-induced self-association of purified tubulin into 42S double rings has been examined by sedimentation velocity. In the absence of magnesium, all complexes sedimented as the 5.8S species. The binding of colchicine to tubulin led to a small b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00149a044
更新日期:1992-09-01 00:00:00
abstract::Fluorescent derivatives of short oligonucleotides of defined sequence were prepared by the incorporation of 5-(propylamino)uridine via current phosphoramidite chemistry, followed by derivatization of the propylamine function with mansyl chloride. These oligomers, annealed to complementary oligomers, yielded short dupl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00437a014
更新日期:1989-05-30 00:00:00
abstract::The human immunodeficiency virus utilizes its gp41 fusion protein to mediate virus-cell membrane fusion. The conserved disulfide loop region in the gp41 hairpin conformation reverses the protein chain, such that the N-terminal heptad repeat and the C-terminal heptad repeat regions interact to form the six-helix bundle...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300868f
更新日期:2012-09-04 00:00:00
abstract::The role of pyridoxal 5'-phosphate (PLP) in the radical-mediated amino group migration catalyzed by lysine 2,3-aminomutase from Clostridia SB4 has been investigated by electron spin echo envelope modulation (ESEEM) spectroscopy. This pulsed electron paramagnetic resonance (EPR) method was used to estimate the distance...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00031a033
更新日期:1995-08-08 00:00:00
abstract::Amyloid deposition of human islet amyloid polypeptide (hIAPP) in the islets of Langerhans is closely associated with the pathogenesis of type II diabetes mellitus. Despite substantial evidence linking amyloidogenic hIAPP to loss of β-cell mass and decreased pancreatic function, the molecular mechanism of hIAPP cytotox...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300542g
更新日期:2012-09-04 00:00:00
abstract::The molecular structure of ammonium deoxycytidylyl-(3'-5')-deoxyguanosine, crystallized from aqueous acetone near pH 4, was determined for X-ray diffraction data. The crystals were tetragonal, space group P43212 with a = b = 11.078 (1) A and c = 45.826 (4) A. The structure was solved by tangent expansion of phases bas...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00277a014
更新日期:1983-04-12 00:00:00
abstract::Binding of mitochondrial cyclophilin (CyP) to the inner mitochondrial membrane is induced by treatment of mitochondria with thiol reagents or oxidative stress and correlates with a sensitization to [Ca2+] of the cyclosporin A-sensitive mitochondrial permeability transition pore (MTP) [Connern, C. P., & Halestrap, A. P...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9525177
更新日期:1996-06-25 00:00:00
abstract::Carbohydrate-binding module (CBM) family 13 includes the "R-type" or "ricin superfamily" beta-trefoil lectins. The C-terminal CBM, CBM13, of xylanase 10A from Streptomyces lividans is a family 13 CBM that is not only structurally similar to the "R-type" lectins but also somewhat functionally similar. The primary funct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015865j
更新日期:2002-04-02 00:00:00
abstract::Nuclear magnetic resonance (NMR) has been used to monitor the conformation and dynamics of the d(C1-G2-A3-T4-T5-A6-T6-A5-A4-T3-C2-G1) self-complementary dodecanucleotide duplex (henceforth called Pribnow 12-mer), which contains a TATAAT Pribnow box and a central core of eight dA X dT base pairs. The exchangeable imino...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00325a019
更新日期:1985-02-12 00:00:00
abstract::Vinca site agents are antimicrotubule compounds that bind to the same site on tubulin as do the vinca alkaloids. These include agents that induce the formation of nonmicrotubule oligomers of tubulin (vinblastine and vincristine) and agents that do not (maytansine and rhizoxin). All of these quench the fluorescence of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00021a012
更新日期:1995-05-30 00:00:00
abstract::The temperature dependence of the hydrogen-tritium and deuterium-hydrogen exchange reactions in poly(DL-alanine) has been reexamined. The results indicate a significant contribution to the observed exchange rates from the water-catalyzed reaction at pD values near pDmin. The activation enthalpy for water-catalyzed deu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00273a031
更新日期:1983-02-15 00:00:00
abstract::Cibacron Blue F3GA, the polycyclic blue chromophore of Blue Dextran 2000, inhibits the catalytic subunit of bovine brain protein kinase. The rate of inactivation exhibits a hyperbolic dependence on the dye concentration. This suggests that an enzyme-dye complex forms prior to inactivation. Protein and peptide substrat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00542a022
更新日期:1980-01-08 00:00:00
abstract::Dynamic conformations of two distinct immunoglobulin (Ig) isotypes, murine IgE and human IgG1, were examined with fluorescence resonance energy transfer measurements. The IgE mutant epsilon/C gamma 3* and the IgG1 mutant gamma/C gamma 3* each bind [5-(dimethylamino)naphthalen-1-yl]sulfonyl (DNS) in two identical antig...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00148a004
更新日期:1992-08-25 00:00:00
abstract::Hirudin is the most potent and specific thrombin inhibitor from medicinal leech with a Ki value of 2.2 x 10(-14) M. It consists of an active site inhibitor segment, hirudin1-48, a fibrinogen-recognition exosite inhibitor segment, hirudin55-65, and a linker, hirudin49-54, connecting these inhibitor segments. The role o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00027a021
更新日期:1995-07-11 00:00:00
abstract::Human immunoglobulin E (IgE) consists of 14 domains, each with the characteristic immunoglobulin fold structure. Compared with the 12-domain structure of immunoglobulin G (IgG), IgE has an additional pair of domains (C epsilon 2) in the Fc region in place of the hinge of IgG. The crystal structure of the 4-domain Fc f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00044a023
更新日期:1995-11-07 00:00:00
abstract::Mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol (Chol) have been used to examine the effects of cholesterol on the chain crystallization transitions and thermomechanical properties in phospholipid bilayer membranes. The mechanical properties--elastic moduli and level of tension at membrane rupture--w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00413a013
更新日期:1988-06-28 00:00:00
abstract::GlgE (EC 2.4.99.16) is an α-maltose 1-phosphate:(1→4)-α-d-glucan 4-α-d-maltosyltransferase of the CAZy glycoside hydrolase 13_3 family. It is the defining enzyme of a bacterial α-glucan biosynthetic pathway and is a genetically validated anti-tuberculosis target. It catalyzes the α-retaining transfer of maltosyl units...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500183c
更新日期:2014-04-22 00:00:00