Abstract：
:Previously we have demonstrated the presence of presynaptic nicotinic acetylcholine receptors on the terminals of myenteric neurons in Auerbach's plexus of guinea-pig ileum. During these studies we observed, that the presence of hemicholinium-3, an inhibitor of the high affinity choline uptake significantly influences the contraction of the longitudinal muscle strip preparation. Our aim was to investigate the neurochemical background of this effect and quantitatively characterize the action of HC-3. We studied the effect of HC-3 on epibatidine- and electrical stimulation-evoked contraction and release of [3H]acetylcholine from the guinea-pig longitudinal muscle strip preparation. We found that in the presence of tetrodotoxin, when the contribution of somatodendritic nicotinic acetylcholine receptors to the response was prevented due to the inhibition of axonal conduction, HC-3 inhibited the epibatidine-evoked contraction and [3H]acetylcholine release in the submicromolar range (IC50 = 897 nM and IC50 = 693 nM, respectively), whereas the electrical stimulation-evoked contraction was not affected by HC-3, and the release of [3H]acetylcholine was apparently enhanced. Our data indicate that HC-3 inhibits the presynaptic nicotinic acetylcholine receptors of myenteric neurons. Since these receptors play an important role in the regulation of cholinergic neurotransmission in the enteric nervous system, the use of HC-3 in [3H]acetylcholine release experiments might bias the interpretation of data.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Mandl P,Kiss JPdoi
10.1016/j.neuint.2006.03.001subject
Has Abstractpub_date
2006-09-01 00:00:00pages
327-33issue
4eissn
0197-0186issn
1872-9754pii
S0197-0186(06)00124-0journal_volume
49pub_type
杂志文章abstract：:Glycine can be substrate for two transporters: GlyT1, largely expressed by astrocytes but also by some non-glycinergic neurons, and GlyT2, most frequently present in glycine-storing nerve endings. In morphological studies, GlyT2 expression had been found to be restricted to caudal regions, being almost undetectable in...
journal_title：Neurochemistry international
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journal_title：Neurochemistry international
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journal_title：Neurochemistry international
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journal_title：Neurochemistry international
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journal_title：Neurochemistry international
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journal_title：Neurochemistry international
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(87)90180-x
更新日期：1987-01-01 00:00:00
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journal_title：Neurochemistry international
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journal_title：Neurochemistry international
pub_type： 杂志文章
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更新日期：2013-11-01 00:00:00
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journal_title：Neurochemistry international
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更新日期：1998-02-01 00:00:00
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journal_title：Neurochemistry international
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journal_title：Neurochemistry international
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journal_title：Neurochemistry international
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journal_title：Neurochemistry international
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journal_title：Neurochemistry international
pub_type： 杂志文章,评审
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journal_title：Neurochemistry international
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journal_title：Neurochemistry international
pub_type： 杂志文章
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2009.10.010
更新日期：2010-01-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章,评审
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更新日期：1995-01-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
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更新日期：1999-12-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
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更新日期：2001-06-01 00:00:00
