Abstract:
:The precise function of activated microglia and their secretory products remains controversial. In order to assess the role of microglial secretion products, we established an in vitro model of an inflammatory reaction in the brain by co-culturing microglial and neuronal cell lines. Upon stimulation with interferon-gamma and lipopolysaccharides, the microglial cells adopted an activated phenotype and secreted tumor necrosis factor-alpha (TNF-alpha), prostaglandin E(2) and nitric oxide (NO). Neuronal degeneration was quantified by measuring the concentrations of microtubule associated protein tau and neuron specific enolase, which are also used as diagnostic tool in Alzheimer's disease, in supernatants. In activated contact co-cultures, the levels of these neuronal markers were significantly raised compared to non-activated co-cultures. NO-synthase inhibitors significantly diminished the rise of tau in activated co-cultures, while indomethacin, superoxide dismutase, or a neutralizing TNF-alpha antibody did not. When a chemical NO-donor or TNF-alpha were added to pure neuronal cultures, cell viability was significantly reduced. TNF-alpha increased neuronal sensitivity towards NO. There were indications that a part of the cells died by apoptosis. This model demonstrates a neurotoxic role for NO in microglia-induced neurodegeneration and provides a valuable in vitro tool for the study of microglia-neuron interactions during inflammation in the brain.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Hemmer K,Fransen L,Vanderstichele H,Vanmechelen E,Heuschling Pdoi
10.1016/s0197-0186(00)00119-4subject
Has Abstractpub_date
2001-06-01 00:00:00pages
557-65issue
7eissn
0197-0186issn
1872-9754pii
S0197018600001194journal_volume
38pub_type
杂志文章abstract::Recent data indicate that BMY 7378 demonstrates high affinity, selectivity and low intrinsic activity at hippocampal 5-HT(1A) receptors, suggesting that BMY 7378 may represent the first selective 5-HT(1A) functional antagonist. The present study examined the agonist and antagonist properties of BMY 7378 at spinal cord...
journal_title:Neurochemistry international
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abstract::Thyrotropin releasing hormone (TRH) present in several brain areas has been proposed as a neuromodulator. Its administration produces opposite effects to those observed with acute ethanol consumption. Opioid peptides, in contrast, have been proposed to mediate some of the effects of alcohol intoxication. We measured T...
journal_title:Neurochemistry international
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journal_title:Neurochemistry international
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abstract::A significant increase in the number of measurable [3H]Ro 5-4864 receptors was found in forebrain membranes of chicks submitted to 15 min of acute swim stress compared to non-stressed chicks. In addition, low subsolubilizing concentrations of Triton X-100 caused a significant increase in the measurable [3H]Ro 5-4864 r...
journal_title:Neurochemistry international
pub_type: 杂志文章
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
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abstract::Neurosteroids are modulators of several receptors and ion channels and are implicated in the pathophysiology of several neuropsychiatric diseases including hepatic encephalopathy (HE). The neurosteroid, allopregnanolone, a positive allosteric modulator of GABA(A) receptors, accumulates in the brains of HE patients whe...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2005.12.006
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abstract::The multidrug transporter P-glycoprotein (P-gp) is expressed in the blood-brain barrier endothelium where it effluxes a range of drug substrates, preventing their accumulation within the brain. P-gp has been studied extensively for 40 years because of its crucial role in the absorption, distribution, metabolism and el...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
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journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(86)90031-8
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journal_title:Neurochemistry international
pub_type: 杂志文章
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journal_title:Neurochemistry international
pub_type: 杂志文章,评审
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更新日期:1995-01-01 00:00:00
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journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(02)00197-3
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journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(93)90094-l
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abstract::The transmitter dopamine and the protein acetylcholinesterase are released within the substantia nigra from the dendrites of nigrostriatal neurons. These phenomena do not correspond to the familiar events involved in information transfer at the classic axons synapse. It is possible that both dopamine and acetylcholine...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(85)90136-6
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abstract::Diabetic neuropathy (DN) is a common form of peripheral neuropathy, yet the mechanisms responsible for chronic pain in this disease are poorly understood. The up-regulation of the expression and function of voltage-gated sodium channel Nav1.7 has been implicated in DN, however, the exact mechanism is unclear. In the p...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2014.05.012
更新日期:2014-09-01 00:00:00
abstract::Ketone bodies serve as a source of energy and as precursors for lipid synthesis in developing brain. Neurons, astrocytes and oligodendroglia all have the necessary enzymes for metabolizing ketone bodies. In this in vivo study it was found that all three cell types were able to use ketone bodies as precursors for the s...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(91)90040-k
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abstract::Synaptic degeneration is one of the earliest hallmarks of Alzheimer disease (AD) and results in loss of cognitive function. One of the causative agents for the synaptic degeneration is the amyloid β-peptide (Aβ), which is formed from its precursor protein by two sequential cleavages mediated by β- and γ-secretase. We ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2012.03.016
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abstract::Phosphodiesterase 7B (PDE7B) inhibition has been considered as a therapeutic target for the treatment of several neurological disorders. Currently, there are no radio-labeled tracers available to determine receptor occupancy (RO) of this target. Developing such a tracer could greatly facilitate the identification of v...
journal_title:Neurochemistry international
pub_type: 杂志文章
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
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abstract::Changes in the level of ornithine decarboxylase mRNA after partial mesodiencephalic hemitransection were evaluated in various regions of the rat brain by means of in situ hybridization histochemistry coupled with computer-assisted image analysis. On days 1 and 2 after the lesion, increased accumulation of ornithine de...
journal_title:Neurochemistry international
pub_type: 杂志文章
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abstract::Epilepsy is a neurological disease recognized as the consequence of excessive neuronal excitability. Endocannabinoid system, the critical regulator of synaptic inhibition in brain, was supposed to be closely involved in epilepsy. Cannabinoid receptors mostly locate on presynaptic terminals of both excitatory and inhib...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
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abstract::A simple procedure for isolating a fraction enriched in synaptic plasma membrane (SPM) vesicles from sheep brain cortex is described and is used in studying uptake and release of [(3)H]GABA. The crude synaptosomal fraction was subjected to osmotic shock and the lysate was subfractioned in a discontinuous density gradi...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(90)90022-l
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abstract::Vinpocetine (ethyl apovincaminate), a synthetic derivative of the Vinca minor alkaloid vincamine, is widely used for the treatment of cerebrovascular-related diseases. One of the proposed mechanisms underlying its action is to protect against the cytotoxic effects of glutamate overexposure. Glutamate excitotoxicity le...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2008.08.003
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abstract::This study aimed to elucidate locomotor activity changes in 6-hydroxydopamine (6-OHDA) induced Parkinson's disease (PD) and investigate the possible beneficial effects of melatonin on altered levels of locomotor activity, cyclooxygenase (COX), prostaglandin E2 (PGE2), nuclear factor kappa-B (NF-κB), nitrate/nitrite an...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2014.09.005
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abstract::Inflammation which is an indispensable participant in tumor progression is intricately linked with redox modulation. The pro-inflammatory cytokine Tumor Necrosis Factor (TNFalpha) elevates reactive oxygen species (ROS) in glioblastoma multiforme (GBM). As both TNFalpha and oxidative stress independently play role in r...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2009.10.003
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abstract::The activity of histochemically demonstrable aspartate aminotransferase was measured histophotometrically in the hippocampal formation, in dorsal root ganglia, and in superior cervical ganglia during postnatal development of the rat. The enzyme activity showed a marked (up to 15 times) increase in the dendritic layers...
journal_title:Neurochemistry international
pub_type: 杂志文章
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abstract::The objectives of this study are to identify the mechanism of mitochondrial dysfunction during cerebral ischemic/reperfusion (I/R) injury and the therapeutic potential of tetrahydrocurcumin (THC) to mitigate mitochondrial dysfunction in experimental stroke model. In our study, 8-10 weeks old male C57BL/6 wild-type mic...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2018.11.015
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abstract::Striatal quinolinic acid lesions have been used as a model for the pathology seen in Huntington's disease. Seven days following a unilateral injection of 100 nmol of quinolinic acid into the rat caudate-putamen (striatum), a large increase in the binding of [3H]WIN 35,428 to the lesioned caudate-putamen was seen when ...
journal_title:Neurochemistry international
pub_type: 杂志文章
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