Abstract:
:A possible modulatory role of kinases on voltage sensitive Na+ channels of presynaptic brain nerve endings was investigated by testing the effect of several kinase activators and inhibitors on the elevation of [Nai] induced by veratridine in mouse brain synaptosomes loaded with a selective Na+ indicator dye. Veratridine (20 microM) increases the basal [Nai] level (20 mM) more than twofold. This increase is independent of external Ca2+, but abolished by tetrodotoxin (1 microM). Activation of cAMP dependent protein kinase with forskolin or cAMP analogs, or of protein kinase C with diacylglycerol did not affect the veratridine-induced elevation in [Nai]. Drugs reported to inhibit calmodulin-dependent events, as well as the regulatory domain of protein kinase C, were potent and effective inhibitors of the increase in [Nai] induced by veratridine, as well as other veratridine induced responses, namely elevation of [Cai] (monitored with the Ca2+ indicator dye fura-2) and neurotransmitter (GABA) release. Drugs that inhibit kinases by binding to the catalytic site were ineffective, however, as was the phosphatase inhibitor, okadaic acid. A selective inhibitor of Ca2+ and calmodulin dependent protein kinase II also did not affect the elevation of [Nai] induced by veratridine, but markedly diminished the elevation of [Cai] induced by depolarization either with veratridine or with high K+ (15 and 30 mM). On the basis of these results it is concluded that, the dramatic inhibition exerted by some of the drugs tested on the elevation of [Nai] induced by veratridine is not due to their effects on kinases, but to a possible interaction of these compounds with an intracellular site of the Na+ channel. On the other hand, while Ca2+ and calmodulin dependent protein kinase II is unable to modulate brain presynaptic voltage sensitive Na+ channels, it facilitates the activation of brain presynaptic voltage sensitive Ca2+ channels.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Sitges M,Peña F,Chiu LM,Guarneros Adoi
10.1016/s0197-0186(97)00065-xsubject
Has Abstractpub_date
1998-02-01 00:00:00pages
177-90issue
2eissn
0197-0186issn
1872-9754pii
S019701869700065Xjournal_volume
32pub_type
杂志文章abstract::Rasagiline is a propargylamine and irreversible monoamine oxidase (MAO) B inhibitor used for the treatment of Parkinson's disease (PD). It has demonstrated neuroprotective properties in laboratory studies. Current concepts of PD aetiopathogenesis include the role of alpha-synuclein, protein aggregation, free radical m...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2010.06.017
更新日期:2010-11-01 00:00:00
abstract::Chronic ingestion of caffeine causes dependence and sleep disturbance in children and adolescents. In rodents, the administration of caffeine may produce behavioral cross-sensitization to some psychostimulants, such as dopaminergic psychoactive drugs. Methylphenidate (MPH; Ritalin) is a psychostimulant used in pediatr...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2009.03.015
更新日期:2009-09-01 00:00:00
abstract::Intracellular Ca2+ signals produced by the glutamate receptor agonist alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA; 5 microM) were measured in the somatic and dendritic regions of cerebellar Purkinje neurons in mature cerebellar control cultures (> or = 20 days in vitro) and cultures chronically treated...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(99)00058-3
更新日期:1999-08-01 00:00:00
abstract::Adenosine exerts two parallel modulatory roles in the CNS, acting as a homeostatic modulator and also as a neuromodulator at the synaptic level. We will present evidence to suggest that these two different modulatory roles are fulfilled by extracellular adenosine originated from different metabolic sources, and involv...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/s0197-0186(00)00034-6
更新日期:2001-02-01 00:00:00
abstract::Lipid peroxidation has many deleterious effects on cells, and in the nervous system is considered to be involved in the pathogenesis of neurodegenerative diseases. To suppress lipid peroxidation, cells have various defense systems such as glutathione and thioredoxin, and defects in these defense systems will result in...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2010.04.015
更新日期:2010-09-01 00:00:00
abstract::Peroxisome proliferator-activated receptors (PPARα, -β/δ and -γ) are lipid-activated transcription factors. Synthetic PPARα and PPARγ ligands have neuroprotective properties. Recently, PPARβ/δ activation emerged as the focus of a novel approach for the treatment of a wide range of neurodegenerative diseases. To fill t...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2013.06.012
更新日期:2013-10-01 00:00:00
abstract::Interleukin-1 beta (IL-1 beta) is a pro-inflammatory cytokine produced in the brain by endogenous microglial cells responding to injury. Levels of IL-1 beta are elevated in several neurodegenerative disorders, including Alzheimer's disease. IL-1 beta, which can act as a mitogen for astrocytes, also elicits the express...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(01)00042-0
更新日期:2001-11-01 00:00:00
abstract::Increased noradrenaline, induced by rapid eye movement (REM) sleep deprivation, stimulates Na-K ATPase activity in the rat brain. The brain contains neurons as well as glia and both possess Na-K ATPase, however, it was not known if REM sleep deprivation affects the enzyme in both types of cells identically. Rats were ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2008.10.006
更新日期:2009-01-01 00:00:00
abstract::The present study has been designed to establish the potential benefits from 1-methylnicotinamide (MNA) treatment on brain disorders associated with type 1 diabetes. All experiments were carried out after 6 weeks of streptozotocin-induced diabetes (60 mg/kg of body weight, i.p.) in male Wistar rats treated for 5 weeks...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2009.10.004
更新日期:2010-01-01 00:00:00
abstract::Using a consistent, reproducible and reliable cortical focal ischemia in rat (permanent unilateral occlusion of the left middle cerebral artery & the ipsilateral common carotid artery [MCAo + CCAo] with a 1 h temporary occlusion of the contralateral CCA), the levels of four major membrane fatty acids (palmitic, C16:0;...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(93)90094-l
更新日期:1993-08-01 00:00:00
abstract::The function of PMP70, one of the four ABC half-transporters of mammalian peroxisomes, encoded by ABCD3 gene, is still unclear. The finding that PMP70 over-expression partially corrected very long-chain fatty acid oxidation defects in fibroblasts of X-linked adrenoleukodystrophy patients, has unveiled its potential cl...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2007.11.007
更新日期:2008-05-01 00:00:00
abstract::Astrocytes, besides supporting metabolic and scaffolding functions, play a prominent role in the modulation of neuronal communication. In particular, they are responsible for clearing synaptically-released glutamate via highly specific transporters located on their plasma membrane. Since glutamate is the main excitato...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2003.07.005
更新日期:2004-07-01 00:00:00
abstract::Quantitative autoradiography analysis of neurotensin (NT) and somatostatin (SS) binding sites was performed on coronal sections of the medulla oblongata from 2 fetuses, 6 controls and 7 victims of Sudden Infant Death Syndrome (SIDS). Throughout the first postnatal year, mean SS binding site density was similar in cont...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(92)90134-d
更新日期:1992-01-01 00:00:00
abstract::This study aimed to elucidate locomotor activity changes in 6-hydroxydopamine (6-OHDA) induced Parkinson's disease (PD) and investigate the possible beneficial effects of melatonin on altered levels of locomotor activity, cyclooxygenase (COX), prostaglandin E2 (PGE2), nuclear factor kappa-B (NF-κB), nitrate/nitrite an...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2014.09.005
更新日期:2014-12-01 00:00:00
abstract::Previously we have demonstrated the presence of presynaptic nicotinic acetylcholine receptors on the terminals of myenteric neurons in Auerbach's plexus of guinea-pig ileum. During these studies we observed, that the presence of hemicholinium-3, an inhibitor of the high affinity choline uptake significantly influences...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2006.03.001
更新日期:2006-09-01 00:00:00
abstract:UNLABELLED:The aggregates of amyloid beta peptides (Aβs) are regarded as one of the main pathological hallmarks of Alzheimer's disease (AD). An imbalance between the rates of synthesis and clearance of Aβs is considered to be a possible cause for the onset of AD. Dipeptidyl peptidases II and IV (DPPII and DPPIV) are se...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2013.03.017
更新日期:2013-06-01 00:00:00
abstract::Antibodies to electrolectin, a lectin endogenous to embryonic skeletal muscle, have been used to study the distribution of lectin during myogenesis in L(6) cells by using immunohistofluorescence microscopy. Evidence for both surface and cytoplasmic forms of lectin is presented. Cytoplasmic lectin does not appear to be...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(80)90041-8
更新日期:1980-01-01 00:00:00
abstract::Cerebral edema is a potentially life-threatening complication shared by diseases of different etiology, such as diabetic ketoacidosis, acute liver failure, high altitude exposure, dialysis disequilibrium syndrome, and salicylate intoxication. Pulmonary edema is also habitually present in these disorders, indicating th...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2012.05.007
更新日期:2012-07-01 00:00:00
abstract::Cerebral ischemia is one of leading causes of death and long-term disability worldwide. Stem cell-based therapy is promising some valuable strategies for the structural and functional recovery after ischemic insult. The inhibition of phosphodiesterases (PDEs) has wide spectrum neuroprotective properties by stimulating...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2019.104630
更新日期:2020-02-01 00:00:00
abstract::Tyrosine hydroxylase (TH) was purified from bovine brain and enzymatically phosphorylated in vitro. Radioactively phosphorylated TH was dephosphorylated by rat tissue extracts. Of tissues examined, rat corpus striatal extracts were highest in specific activity in the TH dephosphorylating assay. Phosphorylated histone ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(83)90016-5
更新日期:1983-01-01 00:00:00
abstract::An investigation was made into the effects of hyperammonemia on the metabolism of brain serotonin (5-HT). The animal model used was the sparse fur (spf) mouse, which possesses an inborn error of the urea cycle, i.e. an abnormal form of ornithine transcarbamylase. Several indoles were measured in brain and plasma using...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(85)90019-1
更新日期:1985-01-01 00:00:00
abstract::The opening of pannexin-1 (Px1) hemichannels is regulated by the activity of P2X(7) receptors (P2X(7)Rs). At present, however, little is known about how extracellular ATP-sensitive P2X(7)Rs regulates the opening and closure of Px1 hemichannels. Several lines of evidence suggest that P2X(7)Rs are activated under pathol...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2010.12.013
更新日期:2011-02-01 00:00:00
abstract::In the present study, we investigated the neuroprotective role of p38 inhibition on experimental stroke in rats. p38 inhibition treatment alleviated the brain infarction volume and neurological deficits following ischemia, promoted the activation of Extracellular signal-regulated kinases (ERK1/2), suppressed the activ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2015.06.010
更新日期:2015-11-01 00:00:00
abstract::The saturable and specific high-affinity uptake of [(3)H]serotonin ([(3)H]5HT) (5 x 10(?8) M) was studied in slices from the hippocampus, parietal cortex, septum-preoptic area, and hypothalamus of male 2, 6, 12 and 24-32 month old C57BL/6N mice. Hippocampal [(3)H]5-HT uptake showed a significant biphasic relationship ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(83)90006-2
更新日期:1983-01-01 00:00:00
abstract::Therapeutic hypothermia as a physical method to lower the brain temperature of patients has been widely used in clinics as an effective and necessary step during the treatment of acute brain injury or edema. However, due to limitations of the ocular structure, the application of hypothermia in retinal neuroprotection ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2019.03.006
更新日期:2019-06-01 00:00:00
abstract::Neurons differentiated in vitro from embryonic stem cells (ESCs) have the potential to serve both as models of disease states and in drug discovery programs. In this study, we use sonic hedgehog (SHH) and fibroblast growth factor 8 (FGF-8) to enrich for forebrain and midbrain phenotypes from mouse ESCs. We then invest...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2011.02.016
更新日期:2011-09-01 00:00:00
abstract::Maple syrup urine disease (MSUD) is an inherited neurometabolic disorder biochemically characterized by the accumulation of the branched-chain alpha-keto acids (BCKA) alpha-ketoisocaproic (KIC), alpha-keto-beta-methylvaleric (KMV) and alpha-ketoisovaleric (KIV) and their respective branched-chain alpha-amino acids in ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2006.05.007
更新日期:2006-12-01 00:00:00
abstract::Zellweger syndrome (ZS) is a neonatal-lethal genetic disease that affects all tissues, and features neuropathology that involves primary developmental defects as well as neurodegeneration. Neuropathological changes include abnormal neuronal migration affecting the cerebral hemispheres, cerebellum and inferior olivary ...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2014.02.007
更新日期:2014-04-01 00:00:00
abstract::Recent in vivo studies have established astrocytes as a major target for locus coeruleus activation (Bekar et al., 2008), renewing interest in cell culture studies on noradrenergic effects on astrocytes in primary cultures and calling for additional information about the expression of adrenoceptor subtypes on differen...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2010.03.019
更新日期:2010-11-01 00:00:00
abstract::Precise anatomical distribution of 5HT1 binding sites has been investigated in the nuclei raphe dorsalis, raphe centralis and locus caeruleus of the rat brain. An original pattern of distribution was observed in the raphe nuclei, closely correlated to the already known distribution of 5HT containing elements. This pat...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(85)90156-1
更新日期:1985-01-01 00:00:00