Phosphorylation of integrin in differentiating ts-Rous sarcoma virus-infected myogenic cells.


:The differentiation of primary myogenic cultures requires the attachment of the cells to an extracellular matrix substrate using an integrin family receptor. These integrin receptors can be phosphorylated on both their alpha and beta chains, and it has been postulated that phosphorylation regulates the receptor function. Quail myogenic clones transformed with ts-LA24A differentiated into mature myotubes following a temperature shift to nonpermissive temperature which inactivates the viral src kinas. Phosphorylation of integrin beta-1 chain and of at least one alpha chain was detected on both serine and tyrosine. An additional alpha chain(s) with a mobility similar to alpha 5 was not phosphorylated at either temperature. Following the induction of differentiation by a temperature shift, there was a marked decrease in integrin phosphorylation of both alpha and beta integrin chains. This decrease was more prominent for serine than for tyrosine, suggesting that src could not be the only kinase involved. The drop in integrin phosphorylation correlated with the initiation of differentiation, suggesting that integrin phosphorylation could be at least part of the mechanism by which myogenic differentiation is blocked by v-src.






Aneskievich BJ,Haimovich B,Boettiger D


Has Abstract


1991-08-01 00:00:00














  • Antiangiogenic and tumour inhibitory effects of downregulating tumour endothelial FABP4.

    abstract::Fatty acid binding protein 4 (FABP4) is a fatty acid chaperone, which is induced during adipocyte differentiation. Previously we have shown that FABP4 in endothelial cells is induced by the NOTCH1 signalling pathway, the latter of which is involved in mechanisms of resistance to antiangiogenic tumour therapy. Here, we...


    pub_type: 杂志文章


    authors: Harjes U,Bridges E,Gharpure KM,Roxanis I,Sheldon H,Miranda F,Mangala LS,Pradeep S,Lopez-Berestein G,Ahmed A,Fielding B,Sood AK,Harris AL

    更新日期:2017-02-16 00:00:00

  • Post-transcriptional inactivation of p53 in immortalized murine embryo fibroblast cells.

    abstract::The steady-state levels of p53 mRNA and protein were barely detectable by Northern and Western blot analysis in spontaneously immortalized (10)3 and (10)7 murine embryo fibroblast (MEF) cells. But when cells were treated with cycloheximide (CHX) or emetine, expression levels were restored to those observed in primary ...


    pub_type: 杂志文章


    authors: Kim H,You S,Farris J,Foster LK,Foster DN

    更新日期:2001-05-31 00:00:00

  • E2F1 suppresses skin carcinogenesis via the ARF-p53 pathway.

    abstract::The E2F1 transcription factor, which is deregulated in most human cancers by mutations in the p16-cyclin D-Rb pathway, has both oncogenic and tumor-suppressive properties. This is dramatically illustrated by the phenotype of an E2F1 transgenic mouse model that spontaneously develops tumors in the skin and other epithe...


    pub_type: 杂志文章


    authors: Russell JL,Weaks RL,Berton TR,Johnson DG

    更新日期:2006-02-09 00:00:00

  • Genetic instability in the 9q22.3 region is a late event in the development of squamous cell carcinoma.

    abstract::Squamous cell carcinoma (SCC) of the skin represents a group of neoplasms which is associated with exposure to UV light. Recently, we obtained data suggesting that invasive skin cancer and its precursors derive from one original neoplastic clone. Here, the analysis were extended by loss of heterozygosity (LOH) analysi...


    pub_type: 杂志文章


    authors: Ahmadian A,Ren ZP,Williams C,Pontén F,Odeberg J,Pontén J,Uhlén M,Lundeberg J

    更新日期:1998-10-08 00:00:00

  • Lung-specific expression of human mutant p53-273H is associated with a high frequency of lung adenocarcinoma in transgenic mice.

    abstract::To investigate the tumorigenic potential of mutant p53 when selectively expressed in lung tissue, a transgenic mouse model was developed in which a mutant form of p53 (p53-273H) was placed under the transcriptional control of the lung-specific human surfactant protein C (SP-C) promoter. Two founder mice were identifie...


    pub_type: 杂志文章


    authors: Duan W,Ding H,Subler MA,Zhu WG,Zhang H,Stoner GD,Windle JJ,Otterson GA,Villalona-Calero MA

    更新日期:2002-11-07 00:00:00

  • Adipocyte-induced CD36 expression drives ovarian cancer progression and metastasis.

    abstract::Ovarian cancer (OvCa) is characterized by widespread and rapid metastasis in the peritoneal cavity. Visceral adipocytes promote this process by providing fatty acids (FAs) for tumour growth. However, the exact mechanism of FA transfer from adipocytes to cancer cells remains unknown. This study shows that OvCa cells co...


    pub_type: 杂志文章


    authors: Ladanyi A,Mukherjee A,Kenny HA,Johnson A,Mitra AK,Sundaresan S,Nieman KM,Pascual G,Benitah SA,Montag A,Yamada SD,Abumrad NA,Lengyel E

    更新日期:2018-04-01 00:00:00

  • Amplification of 11q13 DNA sequences in human breast cancer: D11S97 identifies a region tightly linked to BCL1 which can be amplified separately.

    abstract::In the course of our study on the amplification of 11q13 sequences in human breast cancer, we have investigated the amplification status of the anonymous DNA fragment D11S97 in a series of 125 mammary tumors. Our results indicate that, as with bladder carcinomas, D11S97 can be amplified separately from BCL1. In additi...


    pub_type: 杂志文章


    authors: Szepetowski P,Courseaux A,Carle GF,Theillet C,Gaudray P

    更新日期:1992-04-01 00:00:00

  • Human p53 binds DNA as a protein homodimer but monomeric variants retain full transcription transactivation activity.

    abstract::Wild-type human p53 protein is able to self-associate and consists predominantly of homotetramers in solution. In earlier work we identified the protein sequence motifs involved in p53 quaternary structure and showed that while monomeric p53 protein retains tumour suppressor function, monomeric tumour mutant p53 lacks...


    pub_type: 杂志文章


    authors: Tarunina M,Jenkins JR

    更新日期:1993-11-01 00:00:00

  • The p53 activation and apoptosis induced by DNA damage are reversibly inhibited by salicylate.

    abstract::Treatment of mouse (12)1/CA cells with adriamycin or irradiation with U.V.C. induces p53-dependent transcription of a beta-galactosidase reporter and the endogenous p21/Waf1/Cip1 gene. Despite the induction of Waf1, the cells arrest only transiently in G1 or G2, then resume growth and eventually undergo apoptosis. In ...


    pub_type: 杂志文章


    authors: Chernov MV,Stark GR

    更新日期:1997-05-29 00:00:00

  • MKL1 potentiates lung cancer cell migration and invasion by epigenetically activating MMP9 transcription.

    abstract::Malignant tumors are exemplified by excessive proliferation and aggressive migration/invasion contributing to increased mortality of cancer patients. Matrix metalloproteinase 9 (MMP9) expression is positively correlated with lung cancer malignancy. The mechanism underlying an elevated MMP9 expression is not clearly de...


    pub_type: 杂志文章


    authors: Cheng X,Yang Y,Fan Z,Yu L,Bai H,Zhou B,Wu X,Xu H,Fang M,Shen A,Chen Q,Xu Y

    更新日期:2015-10-29 00:00:00

  • Characterization of a third ras gene, rasB, that is expressed throughout the growth and development of Dictyostelium discoideum.

    abstract::Previous reports have indicated that the cellular slime mold Dictyostelium discoideum possesses two ras genes (rasG and rasD) and one rap gene (rap1). All three genes are developmentally regulated, with each showing a different pattern of transcription during the Dictyostelium life cycle. To establish whether there ar...


    pub_type: 杂志文章


    authors: Daniel J,Spiegelman GB,Weeks G

    更新日期:1993-04-01 00:00:00

  • UDP-glucose 6-dehydrogenase regulates hyaluronic acid production and promotes breast cancer progression.

    abstract::An improved understanding of the biochemical alterations that accompany tumor progression and metastasis is necessary to inform the next generation of diagnostic tools and targeted therapies. Metabolic reprogramming is known to occur during the epithelial-mesenchymal transition (EMT), a process that promotes metastasi...


    pub_type: 杂志文章


    authors: Arnold JM,Gu F,Ambati CR,Rasaily U,Ramirez-Pena E,Joseph R,Manikkam M,San Martin R,Charles C,Pan Y,Chatterjee SS,Den Hollander P,Zhang W,Nagi C,Sikora AG,Rowley D,Putluri N,Zhang XH,Karanam B,Mani SA,Sreekumar A

    更新日期:2020-04-01 00:00:00

  • PMP24, a gene identified by MSRF, undergoes DNA hypermethylation-associated gene silencing during cancer progression in an LNCaP model.

    abstract::Transcriptional silencing of antitumor genes via CpG island methylation could be a mechanism mediating prostate cancer (PCa) progression from an androgen-sensitive (AS) to an androgen-insensitive (AI) state. We have used the methylation-sensitive restriction fingerprinting (MSRF) technique to identify novel CpG-rich s...


    pub_type: 杂志文章


    authors: Wu M,Ho SM

    更新日期:2004-01-08 00:00:00

  • High incidence of medulloblastoma following X-ray-irradiation of newborn Ptc1 heterozygous mice.

    abstract::Individuals affected with the Gorlin syndrome inherit a germ-line mutation of the patched (Ptc1) developmental gene and, analogously to Ptc1 heterozygous mice, show an increased susceptibility to spontaneous tumor development. Human and mouse Ptc1 heterozygotes (Ptc1(+/-)) are also hypersensitive to ionizing radiation...


    pub_type: 杂志文章


    authors: Pazzaglia S,Mancuso M,Atkinson MJ,Tanori M,Rebessi S,Majo VD,Covelli V,Hahn H,Saran A

    更新日期:2002-10-24 00:00:00

  • Increase in specific DNA binding by carboxyl truncation suggests a mechanism for activation of Myb.

    abstract::Oncogenic forms of the c-myb protein (Myb) often exhibit amino-terminal and/or carboxyl-terminal truncations. When the transcriptional activity of these proteins was examined it was found that carboxyl-truncated Myb is more effective as a transcriptional activator than full-length or amino-truncated Myb. In order to d...


    pub_type: 杂志文章


    authors: Ramsay RG,Ishii S,Gonda TJ

    更新日期:1991-10-01 00:00:00

  • Endoglin (CD105): a powerful therapeutic target on tumor-associated angiogenetic blood vessels.

    abstract::Among surface molecules expressed on endothelial cells, endoglin (CD105) is emerging as a prime vascular target for antiangiogenetic cancer therapy. CD105 is a cell membrane glycoprotein mainly expressed on endothelial cells and overexpressed on tumor-associated vascular endothelium, which functions as an accessory co...


    pub_type: 杂志文章,评审


    authors: Fonsatti E,Altomonte M,Nicotra MR,Natali PG,Maio M

    更新日期:2003-09-29 00:00:00

  • Characterization of a major colon cancer susceptibility locus (Ccs3) on mouse chromosome 3.

    abstract::Treatment of mice with the carcinogen azoxymethane (AOM) induces a number of lesions in the colon, including hyperplastic lesions, as well adenomas and carcinomas in situ. Inbred strains of mice show different responses to AOM-induced carcinogenesis. A/J mice are highly susceptible and develop a greater number of hype...


    pub_type: 杂志文章


    authors: Meunier C,Cai J,Fortin A,Kwan T,Marquis JF,Turbide C,Van Der Kraak L,Jothy S,Beauchemin N,Gros P

    更新日期:2010-02-04 00:00:00

  • p53 overexpression is frequent in European hepatocellular carcinoma and largely independent of the codon 249 hot spot mutation.

    abstract::Mutations in the p53 tumour suppressor gene have been recently described in hepatocellular carcinomas (HCC) from high risk areas such as China and South Africa. Our study was designed to assess the importance of p53 aberrations in HCCs from Europe, where the major risk factors in hepatocarcinogenesis, aflatoxin exposu...


    pub_type: 杂志文章


    authors: Volkmann M,Hofmann WJ,Müller M,Räth U,Otto G,Zentgraf H,Galle PR

    更新日期:1994-01-01 00:00:00

  • The E2F5 repressor is an activator of E6/E7 transcription and of the S-phase entry in HPV18-associated cells.

    abstract::High-risk papillomavirus type 18 (HPV18) is one of the less represented HPV types in low-grade lesions of the anogenital tract, whereas it occupies the second place in cervical cancer, where it can be found in 16% of the cases worldwide, after HPV16 present in 54% of them. These epidemiological data indicate that HPV1...


    pub_type: 杂志文章


    authors: Teissier S,Pang CL,Thierry F

    更新日期:2010-09-09 00:00:00

  • Molecular characterization and modular analysis of human MyD88.

    abstract::MyD88 was first characterized as a myeloid differentiation primary response gene in mice, activated in M1 myeloleukemic cells following interleukin-6 (IL-6) induced growth arrest and terminal differentiation. Analysis of expressed sequence tags (ESTs) from activated dendritic cell libraries led to the indentification ...


    pub_type: 杂志文章


    authors: Hardiman G,Rock FL,Balasubramanian S,Kastelein RA,Bazan JF

    更新日期:1996-12-05 00:00:00

  • CXCR7/CXCR4 heterodimer-induced histone demethylation: a new mechanism of colorectal tumorigenesis.

    abstract::Both chemokine receptors (CXCRs) 7 and 4 can facilitate immune cell migration and mediate a vast array of physiological and pathological events. Herein we report, in both human and animal studies, that these two CXCRs can form heterodimers in vivo and promote colorectal tumorigenesis through histone demethylation. Com...


    pub_type: 杂志文章


    authors: Song ZY,Wang F,Cui SX,Gao ZH,Qu XJ

    更新日期:2019-02-01 00:00:00

  • Integrin-linked kinase: not so 'pseudo' after all.

    abstract::Integrin-linked kinase (ILK) is a highly evolutionarily conserved intracellular protein that was originally identified as an integrin-interacting protein, and extensive genetic and biochemical studies have shown that ILK expression is vital during both embryonic development and tissue homeostasis. At the cellular and ...


    pub_type: 杂志文章,评审


    authors: Hannigan GE,McDonald PC,Walsh MP,Dedhar S

    更新日期:2011-10-27 00:00:00

  • BCL11B functionally associates with the NuRD complex in T lymphocytes to repress targeted promoter.

    abstract::BCL11 genes play crucial roles in lymphopoiesis and have been associated with hematopoietic malignancies. Specifically, disruption of the BCL11B (B-cell chronic lymphocytic leukemia/lymphoma 11B) locus is linked to T-cell acute lymphoblastic leukemia, and the loss of heterozygosity in mice results in T-cell lymphoma. ...


    pub_type: 杂志文章


    authors: Cismasiu VB,Adamo K,Gecewicz J,Duque J,Lin Q,Avram D

    更新日期:2005-10-13 00:00:00

  • Apc and p53 interaction in DNA damage and genomic instability in hepatocytes.

    abstract::Disruption of Apc (adenomatous polyposis coli) within hepatocytes activates Wnt signalling, perturbs differentiation and ultimately leads to neoplasia. Apc negatively regulates Wnt signalling but is also involved in organizing the cytoskeleton and may have a role in chromosome segregation. In vitro studies have implic...


    pub_type: 杂志文章


    authors: Méniel V,Megges M,Young MA,Cole A,Sansom OJ,Clarke AR

    更新日期:2015-07-30 00:00:00

  • PTBP3 splicing factor promotes hepatocellular carcinoma by destroying the splicing balance of NEAT1 and pre-miR-612.

    abstract::Nuclear-enriched RNA-binding proteins (RBPs) are mainly involved in transcriptional regulation, which is a critical checkpoint to tune gene diversity and expression levels. We analyzed nuclear RBPs in human HCC tissues and matched normal control tissues. Based on the gene expression levels, PTBP3 was identified as top...


    pub_type: 杂志文章


    authors: Yang X,Qu S,Wang L,Zhang H,Yang Z,Wang J,Dai B,Tao K,Shang R,Liu Z,Li X,Zhang Z,Xia C,Ma B,Liu W,Li H,Dou K

    更新日期:2018-12-01 00:00:00

  • Mechanism of constitutive activation of FLT3 with internal tandem duplication in the juxtamembrane domain.

    abstract::Internal tandem duplication (ITD) of the juxtamembrane (JM) domain of FLT3 is the most frequent mutation in human acute myeloid leukemia, and is significantly associated with leukocytosis and a poor prognosis. Previously we reported that FLT3 with ITD (FLT3/ITD) formed a homodimer and was autophosphorylated on tyrosin...


    pub_type: 杂志文章


    authors: Kiyoi H,Ohno R,Ueda R,Saito H,Naoe T

    更新日期:2002-04-11 00:00:00

  • Expression of alpha-catenin in alpha-catenin-deficient cells results in a reduced proliferation in three-dimensional multicellular spheroids but not in two-dimensional monolayer cultures.

    abstract::alpha-Catenin is an intracellular protein that associates with the carboxy-terminal region of cadherin, a cell adhesion molecule, via beta-catenin or gamma-catenin (plakoglobin). Linkage of cadherin to the cytoskeleton by catenins is required for full cadherin activity. Following transfection of an alpha-catenin-defic...


    pub_type: 杂志文章


    authors: Matsubara S,Ozawa M

    更新日期:2004-04-08 00:00:00

  • ts BCR-ABL kinase activation confers increased resistance to genotoxic damage via cell cycle block.

    abstract::Using a temperature-sensitive mutant of the p210 BCR-ABL gene, transfected into a growth factor-dependent cell line (BaF3), we show that transient BCR-ABL kinase expression increases single cell and clonogenic resistance to apoptosis arising from genotoxic damage induced by ionizing radiation and VP-16/etoposide. This...


    pub_type: 杂志文章


    authors: Nishii K,Kabarowski JH,Gibbons DL,Griffiths SD,Titley I,Wiedemann LM,Greaves MF

    更新日期:1996-11-21 00:00:00

  • Ras regulates kinesin 13 family members to control cell migration pathways in transformed human bronchial epithelial cells.

    abstract::We show that expression of the microtubule depolymerizing kinesin KIF2C is induced by transformation of immortalized human bronchial epithelial cells (HBEC) by expression of K-Ras(G12V) and knockdown of p53. Further investigation demonstrates that this is due to the K-Ras/ERK1/2 MAPK pathway, as loss of p53 had little...


    pub_type: 杂志文章


    authors: Zaganjor E,Osborne JK,Weil LM,Diaz-Martinez LA,Gonzales JX,Singel SM,Larsen JE,Girard L,Minna JD,Cobb MH

    更新日期:2014-11-20 00:00:00

  • DNA adduct burden and tobacco carcinogenesis.

    abstract::DNA adducts associated with tobacco smoking could provide a marker of biologically effective dose of tobacco carcinogens and improve individual cancer risk prediction. A significant number of clinical and epidemiologic studies have reported associations of increased DNA adduct levels with the occurrence of the prevale...


    pub_type: 杂志文章,评审


    authors: Wiencke JK

    更新日期:2002-10-21 00:00:00