E2F regulates DDB2: consequences for DNA repair in Rb-deficient cells.

Abstract:

:DDB2, a gene mutated in XPE patients, is involved in global genomic repair especially the repair of cyclobutane pyrimidine dimers (CPDs), and is regulated by p53 in human cells. We show that DDB2 is expressed in mouse tissues and demonstrate, using primary mouse epithelial cells, that mouse DDB2 is regulated by E2F transcription factors. Retinoblastoma (Rb), a tumor suppressor critical for the control of cell cycle progression, regulates E2F activity. Using Cre-Lox technology to delete Rb in primary mouse hepatocytes, we show that DDB2 gene expression increases, leading to elevated DDB2 protein levels. Furthermore, we show that endogenous E2F1 and E2F3 bind to DDB2 promoter and that treatment with E2F1-antisense or E2F1-small interfering RNA (siRNA) decreases DDB2 transcription, demonstrating that E2F1 is a transcriptional regulator for DDB2. This has consequences for global genomic repair: in Rb-null cells, where E2F activity is elevated, global DNA repair is increased and removal of CPDs is more efficient than in wild-type cells. Treatment with DDB2-siRNA decreases DDB2 expression and abolishes the repair phenotype of Rb-null cells. In summary, these results identify a new regulatory pathway for DDB2 by E2F, which does not require but is potentiated by p53, and demonstrate that DDB2 is involved in global repair in mouse epithelial cells.

journal_name

Oncogene

journal_title

Oncogene

authors

Prost S,Lu P,Caldwell H,Harrison D

doi

10.1038/sj.onc.1210151

subject

Has Abstract

pub_date

2007-05-24 00:00:00

pages

3572-81

issue

24

eissn

0950-9232

issn

1476-5594

pii

1210151

journal_volume

26

pub_type

杂志文章

相关文献

ONCOGENE文献大全
  • The human VE-cadherin promoter is subjected to organ-specific regulation and is activated in tumour angiogenesis.

    abstract::Vascular endothelial (VE)-cadherin is exclusively expressed at interendothelial junctions of normal and tumour vessels. In this report, we characterized the transcriptional activity of the human VE-cadherin promoter. Transient transfection assays revealed that sequences at positions --1135/-744 and -166/-5 base pairs ...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1208483

    authors: Prandini MH,Dreher I,Bouillot S,Benkerri S,Moll T,Huber P

    更新日期:2005-04-21 00:00:00

  • Constitutive activation of Stat3 by the Src and JAK tyrosine kinases participates in growth regulation of human breast carcinoma cells.

    abstract::Constitutive activation of signal transducer and activator of transcription (STAT) proteins has been detected in a wide variety of human primary tumor specimens and tumor cell lines including blood malignancies, head and neck cancer, and breast cancer. We have previously demonstrated a high frequency of Stat3 DNA-bind...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1204349

    authors: Garcia R,Bowman TL,Niu G,Yu H,Minton S,Muro-Cacho CA,Cox CE,Falcone R,Fairclough R,Parsons S,Laudano A,Gazit A,Levitzki A,Kraker A,Jove R

    更新日期:2001-05-03 00:00:00

  • CXCR7/CXCR4 heterodimer-induced histone demethylation: a new mechanism of colorectal tumorigenesis.

    abstract::Both chemokine receptors (CXCRs) 7 and 4 can facilitate immune cell migration and mediate a vast array of physiological and pathological events. Herein we report, in both human and animal studies, that these two CXCRs can form heterodimers in vivo and promote colorectal tumorigenesis through histone demethylation. Com...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/s41388-018-0519-2

    authors: Song ZY,Wang F,Cui SX,Gao ZH,Qu XJ

    更新日期:2019-02-01 00:00:00

  • Hexokinase II: cancer's double-edged sword acting as both facilitator and gatekeeper of malignancy when bound to mitochondria.

    abstract::A key hallmark of many cancers, particularly the most aggressive, is the capacity to metabolize glucose at an elevated rate, a phenotype detected clinically using positron emission tomography (PET). This phenotype provides cancer cells, including those that participate in metastasis, a distinct competitive edge over n...

    journal_title:Oncogene

    pub_type: 杂志文章,评审

    doi:10.1038/sj.onc.1209603

    authors: Mathupala SP,Ko YH,Pedersen PL

    更新日期:2006-08-07 00:00:00

  • p53 represses SV40 transcription by preventing formation of transcription complexes.

    abstract::There is now much evidence to suggest that the p53 tumour suppressor protein functions to monitor the integrity of the genome. When DNA damage is detected, p53 suppresses cell growth to allow repair or directs the cell into apoptosis. The mechanism of action of p53 is as yet unclear but recent evidence has accumulated...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:

    authors: Perrem K,Rayner J,Voss T,Sturzbecher H,Jackson P,Braithwaite A

    更新日期:1995-10-05 00:00:00

  • The many faces of beta-TrCP E3 ubiquitin ligases: reflections in the magic mirror of cancer.

    abstract::Beta-transducin repeats-containing proteins (beta-TrCP) serve as the substrate recognition subunits for the SCFbeta-TrCP E3 ubiquitin ligases. These ligases ubiquitinate specifically phosphorylated substrates and play a pivotal role in the regulation of cell division and various signal transduction pathways, which, in...

    journal_title:Oncogene

    pub_type: 杂志文章,评审

    doi:10.1038/sj.onc.1207389

    authors: Fuchs SY,Spiegelman VS,Kumar KG

    更新日期:2004-03-15 00:00:00

  • The uric acid transporter SLC2A9 is a direct target gene of the tumor suppressor p53 contributing to antioxidant defense.

    abstract::Only humans and higher primates have high uric acid blood levels. Although high uric acid causes gout, it has been linked with human longevity because of its hypothetical antioxidant function. Recent studies reveal that p53 has significant roles in cellular metabolism. One example of this is an antioxidant function th...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/onc.2014.119

    authors: Itahana Y,Han R,Barbier S,Lei Z,Rozen S,Itahana K

    更新日期:2015-04-02 00:00:00

  • Oncogenic activation of c-Abl in non-small cell lung cancer cells lacking FUS1 expression: inhibition of c-Abl by the tumor suppressor gene product Fus1.

    abstract::In lung cancer, frequent loss of one allele of chromosome 3p is seen in both small cell lung cancer and non-small cell lung cancer (NSCLC), providing evidence of tumor suppressor genes (TSGs) in this chromosomal region. The mechanism of Fus1 tumor suppressor activity is unknown. We have found that a Fus1 peptide inhib...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1210500

    authors: Lin J,Sun T,Ji L,Deng W,Roth J,Minna J,Arlinghaus R

    更新日期:2007-10-25 00:00:00

  • Silibinin inhibits hypoxia-inducible factor-1alpha and mTOR/p70S6K/4E-BP1 signalling pathway in human cervical and hepatoma cancer cells: implications for anticancer therapy.

    abstract::The hypoxia-inducible factor 1 (HIF-1) plays a critical role for tumour adaptation to microenvironmental hypoxia, and represents an appealing chemotherapeutic target. Silibinin is a nontoxic flavonoid reported to exhibit anticancer properties. However, the mechanisms by which silibinin inhibits tumour growth are not f...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/onc.2008.398

    authors: García-Maceira P,Mateo J

    更新日期:2009-01-22 00:00:00

  • UVB-induced COX-2 expression requires histone H3 phosphorylation at Ser10 and Ser28.

    abstract::Cyclooxygenase-2 (COX-2) is an inducible enzyme that contributes to the generation of chronic inflammation in response to chemical carcinogens and environmental stresses, including ultraviolet B (UVB) irradiation. Although post-translational histone modifications are believed to have an important role in modulating tr...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/onc.2012.71

    authors: Keum YS,Kim HG,Bode AM,Surh YJ,Dong Z

    更新日期:2013-01-24 00:00:00

  • MEMO1, a new IRS1-interacting protein, induces epithelial-mesenchymal transition in mammary epithelial cells.

    abstract::MEMO1 (mediator of ErbB2-driven cell motility 1) regulates HER2-dependent cell migration. Increased MEMO1 expression is associated with cancer aggressiveness. Here, we found that MEMO1 is also involved in breast carcinogenesis via regulating insulin-like growth factor-I receptor-dependent signaling events. We showed t...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/onc.2012.327

    authors: Sorokin AV,Chen J

    更新日期:2013-06-27 00:00:00

  • Interleukin-7 inhibits apoptosis of mouse malignant T-lymphoma cells by both suppressing the CPP32-like protease activation and inducing the Bcl-2 expression.

    abstract::Mouse malignant T-lymphoma CS-21 cells grow in vitro in the presence of CA-12 lymph node stromal cells, but they undergo apoptotic cell death when separated from CA-12 stromal cells. In the course of examining the nursing effects of CA-12 stromal cells, we found that these cells provided some soluble factors that supp...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:

    authors: Lee SH,Fujita N,Mashima T,Tsuruo T

    更新日期:1996-11-21 00:00:00

  • Deletions of the p16 gene in pediatric leukemia and corresponding cell lines.

    abstract::The p16 gene (MTS1 or CDK4I) encoding an inhibitor of cyclin-dependent kinase 4 (cdk4), has been reported to be deleted in various tumor cell lines, including lines derived from leukemic cells. The reported frequency of p16 gene loss is much higher in established cell lines than in primary tumor specimens. We investig...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:

    authors: Kees UR,Ranford PR,Hatzis M

    更新日期:1996-05-16 00:00:00

  • Alternative splicing in cancer: implications for biology and therapy.

    abstract::Alternative splicing has critical roles in normal development and can promote growth and survival in cancer. Aberrant splicing, the production of noncanonical and cancer-specific mRNA transcripts, can lead to loss-of-function in tumor suppressors or activation of oncogenes and cancer pathways. Emerging data suggest th...

    journal_title:Oncogene

    pub_type: 杂志文章,评审

    doi:10.1038/onc.2013.570

    authors: Chen J,Weiss WA

    更新日期:2015-01-02 00:00:00

  • DNA double strand break repair and chromosomal translocation: lessons from animal models.

    abstract::The maintenance of genomic stability is one of the most important defenses against neoplastic transformation. This objective must be accomplished despite a constant barrage of spontaneous DNA double strand breaks. These dangerous lesions are corrected by two primary pathways of double strand break repair; non homologo...

    journal_title:Oncogene

    pub_type: 杂志文章,评审

    doi:10.1038/sj.onc.1204767

    authors: Ferguson DO,Alt FW

    更新日期:2001-09-10 00:00:00

  • The t(2;3)(q21;q27) translocation in non-Hodgkin's lymphoma displays BCL6 mutations in the 5' regulatory region and chromosomal breakpoints distant from the gene.

    abstract::The BCL6 gene, mapped at the chromosomal band 3q27, encodes a POZ/Zinc finger transcription repressor protein. It is frequently activated in Non-Hodgkin's lymphomas (NHL) by translocations with breakpoints clustering in the 5' major breakpoint region (MBR) as well as by mutations in the same region. The translocations...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1202098

    authors: Chen W,Butler M,Rao PH,Chaganti SR,Louie DC,Dalla-Favera R,Chaganti RS

    更新日期:1998-10-01 00:00:00

  • BARD1 induces apoptosis by catalysing phosphorylation of p53 by DNA-damage response kinase.

    abstract::The BRCA1-associated RING domain protein BARD1 acts with BRCA1 in double-strand break repair and ubiquitination. BARD1 plays a role as mediator of apoptosis by binding to and stabilizing p53, and BARD1-repressed cells are resistant to apoptosis. We therefore investigated the mechanism by which BARD1 induces p53 stabil...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1208491

    authors: Feki A,Jefford CE,Berardi P,Wu JY,Cartier L,Krause KH,Irminger-Finger I

    更新日期:2005-05-26 00:00:00

  • Therapeutic targeting of TP53-mutated acute myeloid leukemia by inhibiting HIF-1α with echinomycin.

    abstract::TP53 mutation in acute myeloid leukemia (AML) is associated with poor prognosis. Since no targeted therapy is available to restore p53 function, it is of great interest to test whether other pathways activated by TP53 mutations can be therapeutically targeted. Here, we showed HIF-1α target genes are enriched in TP53-m...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/s41388-020-1201-z

    authors: Wang Y,Liu Y,Bailey C,Zhang H,He M,Sun D,Zhang P,Parkin B,Baer MR,Zheng P,Malek SN,Liu Y

    更新日期:2020-04-01 00:00:00

  • Involvement of matrix metalloproteinase-13 in stromal-cell-derived factor 1 alpha-directed invasion of human basal cell carcinoma cells.

    abstract::Basal cell carcinoma (BCC) is one of the most common skin neoplasms in humans and is usually characterized by local aggressiveness with little metastatic potential, although deep invasion, recurrence, and regional and distant metastases may occur. Here, we studied the mechanism of BCC invasion. We found that human BCC...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1210040

    authors: Chu CY,Cha ST,Chang CC,Hsiao CH,Tan CT,Lu YC,Jee SH,Kuo ML

    更新日期:2007-04-12 00:00:00

  • The p53 activation and apoptosis induced by DNA damage are reversibly inhibited by salicylate.

    abstract::Treatment of mouse (12)1/CA cells with adriamycin or irradiation with U.V.C. induces p53-dependent transcription of a beta-galactosidase reporter and the endogenous p21/Waf1/Cip1 gene. Despite the induction of Waf1, the cells arrest only transiently in G1 or G2, then resume growth and eventually undergo apoptosis. In ...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1201104

    authors: Chernov MV,Stark GR

    更新日期:1997-05-29 00:00:00

  • Stimulation by hydrogen peroxide of DNA synthesis, competence family gene expression and phosphorylation of a specific protein in quiescent Balb/3T3 cells.

    abstract::Treatment of quiescent Balb/3T3 clone A31-1-1 cells with 0.1-0.2 mM H2O2 in the presence of 1 microM insulin induced DNA synthesis 20-24 h later at almost the same level as that in cells treated with 10% serum. Treatment with 0.1-0.2 mM H2O2 alone did not induce DNA synthesis and was not toxic to the cells. Cell cycle...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:

    authors: Shibanuma M,Kuroki T,Nose K

    更新日期:1990-07-01 00:00:00

  • SASP mediates chemoresistance and tumor-initiating-activity of mesothelioma cells.

    abstract::Here we show that pemetrexed-treated mesothelioma cells undergo accelerated senescence. This is characterized by the secretion of proinflammatory and mitogenic cytokines, reminiscent of an SASP (senescence-associated secretory phenotype). Conditioned media from senescent MPM (malignant pleural mesothelioma) cells trig...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/onc.2011.485

    authors: Canino C,Mori F,Cambria A,Diamantini A,Germoni S,Alessandrini G,Borsellino G,Galati R,Battistini L,Blandino R,Facciolo F,Citro G,Strano S,Muti P,Blandino G,Cioce M

    更新日期:2012-06-28 00:00:00

  • Characterization of changes in gene expression associated with malignant transformation by the NF-kappaB family member, v-Rel.

    abstract::In this study, alterations in gene expression patterns have been examined in v-Rel-transformed avian bone marrow cells. Using a conditional v-Rel estrogen receptor chimera (v-RelER) which transforms cells in an estrogen-dependent manner, we constructed subtraction cDNA libraries from v-RelER-transformed bone marrow ce...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1201334

    authors: Petrenko O,Ischenko I,Enrietto PJ

    更新日期:1997-10-02 00:00:00

  • Adipocyte-induced CD36 expression drives ovarian cancer progression and metastasis.

    abstract::Ovarian cancer (OvCa) is characterized by widespread and rapid metastasis in the peritoneal cavity. Visceral adipocytes promote this process by providing fatty acids (FAs) for tumour growth. However, the exact mechanism of FA transfer from adipocytes to cancer cells remains unknown. This study shows that OvCa cells co...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/s41388-017-0093-z

    authors: Ladanyi A,Mukherjee A,Kenny HA,Johnson A,Mitra AK,Sundaresan S,Nieman KM,Pascual G,Benitah SA,Montag A,Yamada SD,Abumrad NA,Lengyel E

    更新日期:2018-04-01 00:00:00

  • hPMC2 is required for recruiting an ERbeta coactivator complex to mediate transcriptional upregulation of NQO1 and protection against oxidative DNA damage by tamoxifen.

    abstract::In the presence of ERbeta, trans-hydroxytamoxifen (TOT) protects cells against 17beta-estradiol (E(2))-induced oxidative DNA damage (ODD) and this correlates with increased expression of the antioxidative enzyme quinone reductase (QR). Here, we investigate the molecular mechanism responsible for ERbeta-mediated protec...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/onc.2008.235

    authors: Sripathy SP,Chaplin LJ,Gaikwad NW,Rogan EG,Montano MM

    更新日期:2008-10-23 00:00:00

  • MicroRNA-330 acts as tumor suppressor and induces apoptosis of prostate cancer cells through E2F1-mediated suppression of Akt phosphorylation.

    abstract::MicroRNAs (miRNAs) make up a novel class of gene regulators; they function as oncogenes or tumor suppressors by targeting tumor-suppressor genes or oncogenes. A recent study that analysed a large number of human cancer cell lines showed that miR-330 is a potential tumor-suppressor gene. However, the function and molec...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/onc.2009.192

    authors: Lee KH,Chen YL,Yeh SD,Hsiao M,Lin JT,Goan YG,Lu PJ

    更新日期:2009-09-24 00:00:00

  • A peptide that inhibits function of Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) reduces lung cancer metastasis.

    abstract::Myristoylated Alanine-Rich C Kinase Substrate (MARCKS), a substrate of protein kinase C, is a key regulatory molecule controlling mucus granule secretion by airway epithelial cells as well as directed migration of leukocytes, stem cells and fibroblasts. Phosphorylation of MARKCS may be involved in these responses. How...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/onc.2013.336

    authors: Chen CH,Thai P,Yoneda K,Adler KB,Yang PC,Wu R

    更新日期:2014-07-10 00:00:00

  • Rearranged NFKB2 gene in the HUT78 T-lymphoma cell line codes for a constitutively nuclear factor lacking transcriptional repressor functions.

    abstract::Rearrangements of the NFKB2 gene are associated with lymphoid malignancies, but the functional significance of these alterations is not known. Here we characterize structurally and functionally a rearranged NFKB2 gene identified at the T cell lymphoma line, HUT78. The rearrangement has truncated NFKB2 sequences within...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:

    authors: Zhang J,Chang CC,Lombardi L,Dalla-Favera R

    更新日期:1994-07-01 00:00:00

  • Redistribution of CD95, DR4 and DR5 in rafts accounts for the synergistic toxicity of resveratrol and death receptor ligands in colon carcinoma cells.

    abstract::The natural phytoalexin resveratrol (3, 5, 4'-trihydroxystilbene) exhibits both chemopreventive and antitumor activities through a variety of mechanisms. We have shown previously that resveratrol-induced apoptosis of a human colon cancer cell line involved the redistribution of CD95 (Fas/Apo-1) into lipid rafts. Here,...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1208086

    authors: Delmas D,Rébé C,Micheau O,Athias A,Gambert P,Grazide S,Laurent G,Latruffe N,Solary E

    更新日期:2004-11-25 00:00:00

  • Mechanism of constitutive activation of FLT3 with internal tandem duplication in the juxtamembrane domain.

    abstract::Internal tandem duplication (ITD) of the juxtamembrane (JM) domain of FLT3 is the most frequent mutation in human acute myeloid leukemia, and is significantly associated with leukocytosis and a poor prognosis. Previously we reported that FLT3 with ITD (FLT3/ITD) formed a homodimer and was autophosphorylated on tyrosin...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1205332

    authors: Kiyoi H,Ohno R,Ueda R,Saito H,Naoe T

    更新日期:2002-04-11 00:00:00