Abstract:
:A cloned line of S + L- mink lung cells (A clone), which exhibited a flat morphology, was superinfected with a novel dual-tropic virus (E1BX-MuLV) showing a broad host range and a B-tropism. These cells gave rise to transformed cells with two phenotypes: those which were still anchorage-dependent (AD), and those which readily detached spontaneously from the substratum and grew in suspension. A clone of these AD cells (B clone) was isolated and compared with a clone of the anchorage-independent suspension-cultured (AISC) cells (C clone). While the C clone exhibited a high oncogenicity and ability to metastasize in nude mice, the A and B clones were not tumorigenic. The integrated v-mos was greatly amplified in the C clone, and moderately increased in the B clone as compared with the A clone. The amounts of v-mos mRNA expressed by B and C clones paralleled those of v-mos sequence in their chromosomal DNA, whereas there was no detectable v-mos mRNA in the A clone. Thus, conversion of S + L- mink cells from an AD growth to an AISC phenotype accompanied by manifestation of oncogenicity and metastatic potential in nude mice is associated with amplification of integrated v-mos gene and its enhanced expression. Furthermore, a revertant (D clone) showing AD phenotype was derived from the C clone by selective growth in ouabain. This revertant exhibited a markedly decreased oncogenicity in nude mice, although the copy numbers of integrated v-mos gene and its mRNA did not differ from those of the parent C clone. While more p37mos protein was found in the C than in the D clone, it was not detectable in the A and B clone. The amounts of helper virus-related mRNA and infectious E1BX-MuLV were markedly higher in the B than in the C and D clones. It is concluded that v-mos gene amplification and overexpression is necessary for these cells to exhibit oncogenicity, but other factors associated with ouabain-resistance can modify or suppress its oncogenicity despite the v-mos amplification and mRNA overexpression.
journal_name
Oncogenejournal_title
Oncogeneauthors
Gao CL,Wang LC,Vass WC,Seth A,Chang KSsubject
Has Abstractpub_date
1988-09-01 00:00:00pages
267-73issue
3eissn
0950-9232issn
1476-5594journal_volume
3pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::A hallmark of human cancer is heterogeneity, reflecting the complex series of changes resulting in the activation of oncogenes coupled with inactivation of tumor suppressor genes. Breast cancer is no exception and indeed, many studies have revealed considerable complexity and heterogeneity in the population of primary...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.433
更新日期:2012-05-17 00:00:00
abstract::Silencing of the MLH1 gene by promoter hypermethylation is the mechanism underlying the microsatellite instability (MSI) phenotype in endometrial cancers. However, the profile of CpG methylation in a wide range of MLH1 promoters in endometrial cancers and in the normal endometrium is largely unknown. The present study...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206365
更新日期:2003-04-17 00:00:00
abstract::A dominant negative mutant of Ras, M17 Ras, was used to study the role of Ras in receptor coupling of Raf-1 and B-Raf protein serine/threonine kinases (PSKs). We found that mutant Ras blocks serum- and 12-O-tetradecanoyl phorbol 13-acetate-induced activation of Raf-1 kinase in NIH3T3 cells and Raf-1 as well as B-Raf P...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
abstract::Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that modulate key physiological processes ranging from neurotransmission to cancer signaling. These receptors are activated by the neurotransmitter, acetylcholine, and the tobacco alkaloid, nicotine. Recently, the gene cluster encoding the alpha3...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2010.256
更新日期:2010-09-02 00:00:00
abstract::Long-lived species Homo sapiens have evolved robust protection mechanisms against cancer by repressing telomerase and maintaining short telomeres, thereby delaying the onset of the majority of cancer types until post-reproductive age. Indeed, telomerase is silent in most differentiated human cells, predominantly due t...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/s41388-019-0872-9
更新日期:2019-08-01 00:00:00
abstract::Several small GTPases of the Ras superfamily have been shown to antagonize TGFbeta signaling in human tumor cell lines. Some of these GTPases are post-translationally modified by farnesylation, a lipid modification catalyzed by farnesyltransferase and required for the proteins to attach to membranes and to function. I...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203920
更新日期:2000-11-16 00:00:00
abstract::The cyclin-dependent kinase inhibitor p27 is a key regulator of cell-cycle progression. Its expression and localization are altered in several types of malignancies, which has prognostic significance in cancers such as renal cell carcinoma (RCC). S-phase kinase-associated protein 2 (SKP-2) is an F-box protein that is ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.226
更新日期:2013-04-18 00:00:00
abstract::Malignant peripheral nerve sheath tumors (MPNSTs) are soft-tissue sarcomas that frequently arise in patients with neurofibromatosis type 1 (NF1). Most of these tumors are unresectable at diagnosis and minimally responsive to conventional treatment, lending urgency to the identification of new pathway dependencies and ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-019-0965-5
更新日期:2019-09-01 00:00:00
abstract::Eukaryotic translation can be initiated either by a cap-dependent mechanism or by internal ribosome entry, a process by which ribosomes are directly recruited to structured regions of mRNA upstream of the initiation codon. Here we report the finding of an internal ribosome entry site (IRES) in the untranslated region ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206149
更新日期:2003-01-30 00:00:00
abstract::Replacement of leucine 301 in the human colony stimulating factor 1 receptor (CSF-1R) by serine, threonine, glutamic acid, or proline induced ligand-independent transforming activity in mouse NIH3T3 cells, whereas substitution by phenylalanine, methionine, cysteine, or lysine did not. Serine, glutamic acid, and prolin...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::In order to assess the functional contribution of the human c-myc promoter region in the expression of the c-myc gene, transgenic mouse lines containing a bacterial lac Z gene encoding beta-galactosidase under the control of the human c-myc protooncogene promoter were generated. Transgenic mouse embryos heterozygous f...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-06-15 00:00:00
abstract::Leukemic lymphoblasts within different immunophenotypic populations possess stem cell properties. However, whether or not the self-renewal program is retained from stem cells or conferred on progenitors by leukemogenic molecules remains unknown. We have addressed the issue in the context of TEL-AML1-associated acute l...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.148
更新日期:2015-04-16 00:00:00
abstract::Antimicrobial peptides, such as the cathelicidin LL-37/hCAP-18 and its mouse homolog cathelicidin-related antimicrobial peptide (CRAMP), are important effectors of the innate immune system with direct anti-bacterial activity. Cathelicidin is possibly involved in the regulation of tumor cell growth. The aim of this stu...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.248
更新日期:2014-05-22 00:00:00
abstract::Immunoreceptor tyrosine-based activation motifs (ITAMs) are involved in the transduction of signals necessary for activation, differentiation, and survival in hematopoietic cells. Several viruses have been shown to encode ITAM-containing transmembrane proteins. Although expression of these viral proteins has in some c...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209296
更新日期:2006-05-04 00:00:00
abstract::Proliferating cell nuclear antigen (PCNA) has no intrinsic enzymatic function, but functions as a sliding platform to mediate protein interactions with the DNA strand. Many proteins interact with PCNA through a small conserved motif with consensus QxxLxxFF. This work uses Schizosaccharomyces pombe and human cells to a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209320
更新日期:2006-05-11 00:00:00
abstract::Caspase-8 is a key effector of death-receptor-triggered apoptosis. In a previous study, we demonstrated, however, that caspase-8 can also be activated in a death receptor-independent manner via the mitochondrial apoptosis pathway, downstream of caspase-3. Here, we show that caspases-3 and -8 mediate a mitochondrial am...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206280
更新日期:2003-04-17 00:00:00
abstract::Overexpression of hepatocyte growth factor (HGF), also called scatter factor (SF), and its receptor c-Met are associated with poor prognosis for cancer patients. In particular, breast cancer cells can produce HGF that acts in a paracrine as well as in an autocrine manner. Therefore, HGF and c-Met are putative targets ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207063
更新日期:2003-11-20 00:00:00
abstract::To investigate the possible role of the product of the retinoblastoma susceptibility gene, pRB, in leukemogenesis, we examined fresh leukemia cells from 56 cases of primary leukemia (AML, 32; ALL, 12; CML-BC, 9; CLL, 3) for expression of pRB by using an immunoblotting assay with anti-pRB monoclonal antibodies PMG 3-24...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::Mel-18 has been implicated in several processes in tumor progression, in which the Akt pathway is involved as an important key molecular event. However, the function of Mel-18 in human cancers has not been fully established yet. Here, we examined the effect of Mel-18 on tumor angiogenesis in human breast cancer, and f...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.174
更新日期:2011-11-10 00:00:00
abstract::To investigate the tumorigenic potential of mutant p53 when selectively expressed in lung tissue, a transgenic mouse model was developed in which a mutant form of p53 (p53-273H) was placed under the transcriptional control of the lung-specific human surfactant protein C (SP-C) promoter. Two founder mice were identifie...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205909
更新日期:2002-11-07 00:00:00
abstract::Non small cell lung cancer (NSCLC) is the leading cause of cancer deaths in the United States and worldwide. Unfortunately, standard therapies remain inadequate. An increased understanding of the molecular biology of lung cancer biology is required to develop more effective new therapies. In this report, we show that ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206817
更新日期:2003-10-16 00:00:00
abstract::Stimulator of interferon genes (STING) is a cellular sensor that controls cytosolic DNA-activated innate immune signaling. We have previously demonstrated that STING-deficient mice are resistant to carcinogen-induced skin cancer, similar to myeloid differentiation primary response gene 88 (MyD88) deficient mice, since...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.457
更新日期:2015-10-08 00:00:00
abstract::Upregulated gene 19 (U19)/ELL-associated factor 2 (Eaf2) is a potential human tumor suppressor that exhibits frequent allelic loss and downregulation in high-grade prostate cancer. U19/Eaf2, along with its homolog Eaf1, has been reported to regulate transcriptional elongation via interaction with the eleven-nineteen l...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210786
更新日期:2008-03-06 00:00:00
abstract::The NF-kappaB signaling pathway has particular relevance to several liver diseases including hepatitis (liver infection by Helicobacter, viral hepatitis induced by HBV and HCV), liver fibrosis and cirrhosis and hepatocellular carcinoma. Furthermore, the NF-kappaB signaling pathway is a potential target for development...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2008.300
更新日期:2008-10-20 00:00:00
abstract::Radicicol, an inhibitor of Src-family protein-tyrosine kinases, causes morphological reversion of v-src- and v-Ha-ras-transformed fibroblasts and arrest of the cell cycle at both the G1 and the G2 phases. Radicicol was found to inhibit the growth of several other oncogene-transformed cell lines and human carcinoma cel...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201443
更新日期:1997-11-20 00:00:00
abstract::Chromosome 8 loss of heterozygosity (LOH) in cancer of the urinary bladder is associated with high tumour grade and stage. We have screened 193 cases of transitional cell carcinoma (TCC) of the bladder using 30 microsatellite polymorphisms on chromosome 8. Forty three cases (22%) showed LOH on 8p, the majority of whic...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1996-03-07 00:00:00
abstract::Resistance to detachment-induced apoptosis, a process commonly referred as anoikis, is emerging as a hallmark of metastatic malignancies, mainly because it can ensure anchorage-independent growth and survival during organ colonization. Besides, a sustained oxidative stress has been associated with several steps of car...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2009.77
更新日期:2009-05-21 00:00:00
abstract::The phosphatase Cdc25A was shown to be a target of the transcription factor c-Myc. Myc-induced apoptosis appeared dependent on Cdc25A expression and Cdc25A over-expression could substitute for Myc-triggered apoptosis. These findings suggested that an important downstream component of Myc-mediated apoptosis was identif...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204499
更新日期:2001-07-27 00:00:00
abstract::Previous studies have shown that the adenovirus E1A oncoprotein can bind to and inactivate the retinoblastoma tumor suppressor protein (pRb) and the transcriptional coactivators CBP/p300. In this study, wild-type E1A12S or two deletion mutants (delN, which binds pRb but not CBP/p300; delCR2, which binds to CBP/p300 bu...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205050
更新日期:2002-02-07 00:00:00
abstract::Malignant pleural mesothelioma (MPM) is a highly aggressive tumor that is related to asbestos exposure. MPM is characterized by rapid and diffuse local growth in the thoracic cavity, and it has a poor prognosis because it is often refractory to conventional therapy. Although MPM is an extraordinarily challenging disea...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2009.415
更新日期:2010-02-25 00:00:00