Abstract:
:Basic helix-loop-helix (bHLH) transcription factors play a pivotal role in the regulation of tumorigenesis, and also in a wide range of other developmental processes in diverse species from yeast to humans. Here we demonstrate for the first time that Ret finger protein (RFP), a member of the TRIM family of proteins initially identified as a recombined transforming gene from a human lymphoma, is a novel interaction partner for four different bHLH proteins (SCL, E47, MyoD and mASH-1), but does not interact with GATA-1 or PU.1. Interaction with SCL required the B-box and first coiled-coil region of RFP together with the bHLH domain of SCL. RFP was able to repress transcriptional activation by E47, MyoD and mASH-1, but not by members of several other transcription factor families. Transcriptional repression by RFP was trichostatin A sensitive and did not involve an Id-like mechanism or ubiquitination with subsequent degradation of bHLH proteins. Instead, our results suggest that bHLH transcription factors are regulated by a previously undescribed interaction with RFP, which functions to recruit HDAC and/or Polycomb proteins and thus repress target genes of bHLH proteins. These results reveal an unexpected link between the bHLH and TRIM protein families.
journal_name
Oncogenejournal_title
Oncogeneauthors
Bloor AJ,Kotsopoulou E,Hayward P,Champion BR,Green ARdoi
10.1038/sj.onc.1208828subject
Has Abstractpub_date
2005-10-13 00:00:00pages
6729-36issue
45eissn
0950-9232issn
1476-5594pii
1208828journal_volume
24pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::The Ste20-like kinase, SLK, is involved in the control of cell motility through its effects on actin reorganization and focal adhesion turnover. Here we investigated the role of SLK in chemotaxis downstream of the tyrosine kinase receptor, HER2/ErbB2/Neu, which is frequently overexpressed in human breast cancers. Our ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2009.146
更新日期:2009-08-06 00:00:00
abstract::The p53 tumor suppressor protein plays a critical role in mediating cellular response to stress. Upon DNA damage, post-translational modifications stabilize and activate this nuclear phosphoprotein. To determine the effect of phosphorylation site mutants in the context of the whole p53 protein, we performed reporter a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207005
更新日期:2004-01-08 00:00:00
abstract::The oncoprotein c-Myc contains two dimerization motifs- the helix-loop-helix (HLH) and the leucine zipper (LZ) - through which c-Myc specifically dimerizes with Max. We substituted regions of the c-Myc HLH and LZ motifs with the corresponding regions of structurally related proteins that do not interact with Max. Spec...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Diminished expression of the metastasis suppressor protein RKIP was previously reported in a number of cancers. The underlying mechanism remains unknown. Here, we show that the expression of RKIP negatively correlates with that of Snail zinc-transcriptional repressor, a key modulator of normal and neoplastic epithelia...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210860
更新日期:2008-04-03 00:00:00
abstract::Constitutive activation of the signal transducer and activator of transcription 3 (Stat3) and mutation of the p53 are both commonly detected in human prostate cancer cells. We sought to investigate whether there is functional regulation of Stat3 by wild-type (wt) p53. Our results demonstrate that expression of wt p53 ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205426
更新日期:2002-05-02 00:00:00
abstract::The products of the proto-oncogenes c-jun and c-fos are known to form a complex in vivo. Complex formation appears to stabilize protein-DNA interactions and is thought to play an important functional role in transcriptional regulation. Here we show that the viral Jun oncoprotein, which differs structurally from cellul...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::Simian virus 40 (SV40) is a small DNA tumor virus whose early region gene product, large T antigen, is sufficient to immortalize primary rodent cells and transform established rodent cell lines. Three functional domains of large T antigen are required for transformation of the rat embryo fibroblast REF 52 cell line: t...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-12-07 00:00:00
abstract::The introduction of new therapies against particular genetic mutations in non-small-cell lung cancer is a promising avenue for improving patient survival, but the target population is small. There is a need to discover new potential actionable genetic lesions, to which end, non-conventional cancer pathways, such as RN...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2015.469
更新日期:2016-08-18 00:00:00
abstract::BAG-1 is a Hsp70/Hsc70-binding protein that interacts with Bcl-2, Raf-1, steroid hormone receptors, Siah-1, and hepatocyte growth factor (HGF) receptors, implying multiple functions for the BAG-1 protein. Here, we provide evidence that gene transfer-mediated overexpression of BAG-1 markedly enhances the motility of hu...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202661
更新日期:1999-05-27 00:00:00
abstract::Murine double minute (MDM2) binding protein (MTBP) has been implicated in cancer progression. Here, we demonstrate one mechanism by which MTBP inhibits cancer metastasis. Overexpression of MTBP in human osteosarcoma cell lines lacking wild-type p53 did not alter primary tumor growth in mice, but significantly inhibite...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.69
更新日期:2013-01-24 00:00:00
abstract::Human basal-like breast cancer (BLBC) is an enigmatic and aggressive malignancy with a poor prognosis. There is an urgent need to identify therapeutic targets for BLBC, because current treatment modalities are limited and not effective. The forkhead box transcription factor FOXC1 has recently been identified as a crit...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.635
更新日期:2012-11-08 00:00:00
abstract::Although cell invasion is a necessary early step in cancer metastasis, its regulation is not well understood. We have previously shown, in human prostate cancer, that transforming growth factor beta (TGFbeta)-mediated increases in cell invasion are dependent upon activation of the serine/threonine kinase, p38 MAP kina...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209337
更新日期:2006-05-18 00:00:00
abstract::Constitutive activation of the phosphoinositide 3-kinase (PI3K)-AKT pathway is observed in up to 70% of acute myelogenous leukemia. To investigate the relevance of an intrinsic PI3K-AKT pathway activation in hematopoietic malignancies, we analysed the effect of point mutations in the catalytic (p110alpha) and regulato...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2008.40
更新日期:2008-07-03 00:00:00
abstract::The initiation, magnitude and duration of an immune response against antigens are a tightly regulated process involving a dynamic, orchestrated balance of pro- and anti-inflammatory pathways in immune cells. Such a delicate balance is critical for allowing efficient immune response against foreign antigens while preve...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2009.334
更新日期:2010-01-14 00:00:00
abstract::The tumor suppressor gene p53 is a potent transcriptional regulator of genes which are involved in many cellular activities including cell cycle arrest, apoptosis, and angiogenesis. Recent studies have demonstrated that the activation of the transcriptional factor nuclear factor kappaB (NF-kappaB) plays an essential r...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203383
更新日期:2000-02-10 00:00:00
abstract::We compared the mitogenic and signaling pathways of three Fibroblast Growth Factor Receptors (FGFRs), FGFR1, KGFR and FGFR4 in the same cell line. Each receptor was expressed in L6E9 rat myoblasts that do not normally express detectable levels of FGFRs and clones that express comparable levels of each receptor were se...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-04-20 00:00:00
abstract::The Ets family of transcription factors is one of a growing number of master regulators of development. This family was originally defined by the presence of a conserved DNA binding domain, the Ets domain. To date, nearly 30 members of this family have been identified and implicated in a wide range of physiological an...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1204038
更新日期:2000-12-18 00:00:00
abstract::Transcription factors (TFs) coordinate the on-and-off states of gene expression typically in a combinatorial fashion. Studies from embryonic stem cells and other cell types have revealed that a clique of self-regulated core TFs control cell identity and cell state. These core TFs form interconnected feed-forward trans...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/s41388-020-01459-w
更新日期:2020-10-01 00:00:00
abstract::The human ETS2 gene is a member of the ets gene family (ETS1, ETS2, ERG, ELK1 and ELK2) with amino acid similarity to the v-ets oncogene of the avian leukemia virus, E26. The ETS2 gene is composed of 10 exons, nine of which contribute to the open reading frame encoding 469 amino acids. The ETS2 gene directs the synthe...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::A selective switch from expression of Shc1 gene to Shc3 occurs with maturation of neuronal precursors into postmitotic neurons. Previous studies showed that in the embryo, Shc1 is maximally expressed in dividing CNS stem cells while it is silenced in mature neurons, where it is replaced by Shc3. Under normal condition...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208708
更新日期:2005-08-04 00:00:00
abstract::Epithelial-to-mesenchymal transition (EMT) promotes cell motility, which is important for the metastasis of malignant cells, and blocks CD95-mediated apoptotic signaling triggered by immune cells and chemotherapeutic regimens. CD95L, the cognate ligand of CD95, can be cleaved by metalloproteases and released as a solu...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.55
更新日期:2015-02-19 00:00:00
abstract::Pretreatment of cells with 0.5 mM sodium arsenite (but not other activators of stress-activated MAP kinase cascades) prevents the activation of p21Ras and strongly suppresses the activation of c-Raf and the MAP kinase cascade by a variety of growth factors. Arsenite appears to exert its effect by preventing the guanin...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202168
更新日期:1998-07-09 00:00:00
abstract::Primary neonatal Balb/c keratinocyte (NEK) cultures grown, using 3T3 feeder cell support in high calcium, serum supplemented medium, were transfected with EJ-Ha-ras or v-fos DNA sequences and pSV2 neo. Several neo resistant clones were isolated and several established cell lines expressing the transfected gene product...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::Amplified in breast cancer 1 (AIB1) is a transcriptional coactivator for nuclear receptors and other transcription factors. AIB1 has an important role in malignancy of several cancers such as breast and prostate cancers. However, its involvement in human hepatocellular carcinoma (HCC) progression remains unclear. Here...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.90
更新日期:2010-06-10 00:00:00
abstract::Guanine nucleotide exchange factors from the Dbl family are proto-oncogenic proteins that activate small GTPases of the Rho family. Here we report the characterization of GEF720, a novel Dbl-like protein related to p115Rho-GEF. GEF720 activated RhoA both in our recently developed Yeast Exchange Assay and in biochemica...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204921
更新日期:2001-11-01 00:00:00
abstract::The PI3K/AKT signaling pathway is aberrant in a wide variety of cancers. Downstream effectors of AKT are involved in survival, growth and metabolic-related pathways. In contrast, contradictory data relating to AKT effects on cell motility and invasion, crucial prometastatic processes, have been reported pointing to a ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.421
更新日期:2011-01-27 00:00:00
abstract::A single heat shock factor (HSF), mediating the heat shock response, exists from yeast to Drosophila, whereas several related HSFs have been found in mammals. This raises the question of the specific or redundant functions of the different members of the HSF family and in particular of HSF1 and HSF2, which are both ub...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.171
更新日期:2010-07-22 00:00:00
abstract::In this study we have investigated DNA-protein interactions at an AP1-like motif of the neuropeptide tyrosine (NPY) promoter during in vitro differentiation of human neuroblastoma cells SH-SY5Y to mature nonproliferative sympathetic neuron-like cells. These neuroblast-like cells originate from the parental cell line S...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::We previously reported that YY-1, a versatile transcription factor, regulates expression of glycophorin gene by binding to its locus control region-like region (Gp-LCR) in combination with E-box binding protein during murine erythroleukemia (MEL) cell differentiation. In the present work, we demonstrated that YY-1 and...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202026
更新日期:1998-08-27 00:00:00
abstract::We have established conditions for the immortalization of human fibroblasts by the large T antigen of the rodent virus polyoma. This allows the mechanism of immortalization to be studied, without interference by transformation events, in cells with relatively stable chromosomes. Large T antigen could immortalize human...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00