Starvation of a clonal osteoblast-like cell line, MOB 3-4-F2, down-regulates prostaglandin E2 receptors but increases cAMP response to prostaglandin E2.


:Prostaglandin E2 (PGE2) stimulated cAMP production in the MOB 3-4-F2 cell line, a subclone of the osteoblast-like MOB 3-4 cell line. After being cultured in alpha-minimum essential medium supplemented with 10% heat-inactivated foetal calf serum (HIFCS), cells responded to PGE2 (greater than or equal to 50 ng/ml) with a small, but significant, increase in cAMP production. This response did not vary with duration of culture. In 2% HIFCS-containing medium, despite their lower basal cAMP level, cells responded to PGE2 (greater than or equal to 5 ng/ml) with strikingly increased cAMP production. In addition, prolonged culture in this serum-deficient medium enhanced this response. On the other hand, culture of cells in 2% HIFCS-containing medium decreased the apparent number of PGE2 receptors, which was also enhanced by prolonged culture, without effect on their apparent affinity. Their number in 10% HIFCS-containing medium, more than that in 2% HIFCS-containing medium, was almost constant, independent of the culture period. Starvation of MOB 3-4-F2 cells in serum-deficient medium, therefore, appeared to down-regulate PGE2 receptors but increase the cAMP response to PGE2. Moreover, prolonged starvation of cells appeared to facilitate these phenomena. Our findings suggest that cAMP response to PGE2 does not always reflect the number of available PGE2 receptors in the cells.


Cell Signal


Cellular signalling


Kawase T,Orikasa M,Suzuki A




Has Abstract


1991-01-01 00:00:00














  • The upstream stimulatory factor USF1 is regulated by protein kinase CK2 phosphorylation.

    abstract::The upstream stimulatory factors 1 (USF1) and 2 (USF2) are transcription factors which bind to E-box motifs of various promoters regulating a variety of different cellular processes. Only little is known about the regulation of USFs. Here, we identified protein kinase CK2 as an enzyme that phosphorylates USF1 but not ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Lupp S,Götz C,Khadouma S,Horbach T,Dimova EY,Bohrer AM,Kietzmann T,Montenarh M

    更新日期:2014-12-01 00:00:00

  • Monocyte chemotactic protein-induced protein (MCPIP) promotes inflammatory angiogenesis via sequential induction of oxidative stress, endoplasmic reticulum stress and autophagy.

    abstract::Major diseases such as cardiovascular diseases, rheumatoid arthritis, diabetes, obesity and tumor growth are known to involve inflammation. Inflammatory molecules such as MCP-1, TNF-α, IL-1β and IL-8 are known to promote angiogenesis. MCP-induced protein (MCPIP), originally discovered as a novel zinc finger protein in...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Roy A,Kolattukudy PE

    更新日期:2012-11-01 00:00:00

  • Overexpression of dJmj differentially affects intestinal stem cells and differentiated enterocytes.

    abstract::Jumonji (Jmj)/Jarid2 is a DNA-binding transcriptional repressor mediated via histone methylation. Nevertheless, the well-known function of Jmj is as a scaffold for the recruitment of various complexes including Polycomb repressive complex 2 (PRC2), and required for mouse embryonic stem cell development. However, PRC2 ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Suong DNA,Shimaji K,Pyo JH,Park JS,Yoshida H,Yoo MA,Yamaguchi M

    更新日期:2018-01-01 00:00:00

  • Curcumin represses mTORC1 signaling in Caco-2 cells by a two-sided mechanism involving the loss of IRS-1 and activation of AMPK.

    abstract::The mechanistic target of rapamycin complex 1 (mTORC1) is a central modulator of inflammation and tumorigenesis in the gastrointestinal tract. Growth factors upregulate mTORC1 via the PI3K/AKT and/or Ras/MAPK signal pathways. Curcumin (CUR), a polyphenol found in turmeric roots (Curcuma longa) can repress mTORC1 kinas...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Kaur H,Moreau R

    更新日期:2021-02-01 00:00:00

  • Long non-coding RNA DANCR promotes colorectal tumor growth by binding to lysine acetyltransferase 6A.

    abstract::Recent studies have demonstrated that long non-coding RNAs (lncRNAs) play critical roles in cancer development and progression. However, the mechanism by which lncRNAs contribute to colorectal cancer remains unclear. In this study, we identified the lncRNA, DANCR, which was upregulated in colorectal cancer. The upregu...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Lian J,Zhang H,Wei F,Li Q,Lu Y,Yu B,Yu L,Liang X,Wen Y,Jin K,Tang J,Xie W

    更新日期:2020-03-01 00:00:00

  • Regulation of endothelial migration and proliferation by ephrin-A1.

    abstract::Endothelial migration and proliferation are fundamental processes in angiogenesis and wound healing of injured or inflamed vessels. The present study aimed to investigate the regulation of the Eph/ephrin-system during endothelial proliferation and the impact of the ligand ephrin-A1 on proliferation and migration of hu...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Wiedemann E,Jellinghaus S,Ende G,Augstein A,Sczech R,Wielockx B,Weinert S,Strasser RH,Poitz DM

    更新日期:2017-01-01 00:00:00

  • Examination of the expanding pathways for the regulation of p21 expression and activity.

    abstract::p21(Waf1/Cip1/Sdi1) was originally identified as an inhibitor of cyclin-dependent kinases, a mediator of p53 in growth suppression and a marker of cellular senescence. p21 is required for proper cell cycle progression and plays a role in cell death, DNA repair, senescence and aging, and induced pluripotent stem cell r...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Jung YS,Qian Y,Chen X

    更新日期:2010-07-01 00:00:00

  • Actinomycin D and nutlin-3a synergistically promote phosphorylation of p53 on serine 46 in cancer cell lines of different origin.

    abstract::The p53 tumor suppressor protein is a transcription factor activated by phosphorylation of its N-terminus. MDM2, encoded by a p53-activated gene, acts as a negative-feedback regulator of p53 by promoting p53 degradation. Moreover, MDM2 inhibits p53 by binding to and concealing its N-terminal transcription-activating d...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Zajkowicz A,Gdowicz-Kłosok A,Krześniak M,Ścieglińska D,Rusin M

    更新日期:2015-09-01 00:00:00

  • The last five amino acid residues at the C-terminus of PRK1/PKN is essential for full lipid responsiveness.

    abstract::PRK1/PKN is a member of the protein kinase C (PKC) superfamily of serine/threonine protein kinases. Despite its important role as a RhoA effector, limited information is available regarding how this kinase is regulated. We show here that the last seven amino acid residues at the C-terminus is dispensable for the catal...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Lim WG,Zhu Y,Wang CH,Tan BJ,Armstrong JS,Dokland T,Yang H,Zhu YZ,Teo TS,Duan W

    更新日期:2005-09-01 00:00:00

  • Activin induces hepatocyte cell growth arrest through induction of the cyclin-dependent kinase inhibitor p15INK4B and Sp1.

    abstract::In this report, we examined the role of activin in the regulation of cell growth inhibition of human hepatocarcinoma cells. Using RNase protection assay for various cell cycle regulators and Western blotting experiments, we show that activin treatment of HepG2 cells leads to increased gene expression of the cyclin-dep...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Ho J,de Guise C,Kim C,Lemay S,Wang XF,Lebrun JJ

    更新日期:2004-06-01 00:00:00

  • Guanine nucleotide exchange factors for RhoGTPases: good therapeutic targets for cancer therapy?

    abstract::Rho guanosine triphosphatases (GTPases) are a family of small proteins which function as molecular switches in a variety of signaling pathways following stimulation of cell surface receptors. RhoGTPases regulate numerous cellular processes including cytoskeleton organization, gene transcription, cell proliferation, mi...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Lazer G,Katzav S

    更新日期:2011-06-01 00:00:00

  • Role of nitric oxide produced by iNOS through NF-κB pathway in migration of cerebellar granule neurons induced by Lipopolysaccharide.

    abstract::Inflammatory stimulus during development increases the risk for adverse neurologic outcome. One possible mechanism is disrupting neuronal migration. Using lipopolysaccharide (LPS)-treatment to assess inflammatory stimulus on neuronal migration of cerebellar granule neurons, we previously found that LPS-activation incr...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Arias-Salvatierra D,Silbergeld EK,Acosta-Saavedra LC,Calderon-Aranda ES

    更新日期:2011-02-01 00:00:00

  • Sphingosine kinase/sphingosine 1-phosphate signalling in central nervous system.

    abstract::Sphingolipids were once regarded as inert structural components of cell membranes. Now these metabolites are generally believed to be important bioactive molecules that control a wide repertoire of cellular processes such as proliferation and survival of cells. Along with these ubiquitous cell functions observed in ma...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Okada T,Kajimoto T,Jahangeer S,Nakamura S

    更新日期:2009-01-01 00:00:00

  • UNC119 inhibits dynamin and dynamin-dependent endocytic processes.

    abstract::Unc119 is an adapter signaling molecule, which regulates activation of tyrosine kinases in T cells, eosinophils and fibroblasts. It plays an important role in the photoreceptor synapses of the retina. Recently, we have shown that it inhibits bacterial uptake through macropinocytosis. In this paper we demonstrate a rol...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Karim Z,Vepachedu R,Gorska M,Alam R

    更新日期:2010-01-01 00:00:00

  • Calcium-calmodulin-dependent kinase II (CaMKII) mediates insulin-stimulated proliferation and glucose uptake.

    abstract::Cellular growth and glucose uptake are regulated by multiple signals generated by the insulin receptor. The mechanisms of individual modulation of these signals remain somewhat elusive. We investigated the role of CaMKII in insulin signalling in a rat skeletal muscle cell line, demonstrating that CaMKII modulates the ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Illario M,Monaco S,Cavallo AL,Esposito I,Formisano P,D'Andrea L,Cipolletta E,Trimarco B,Fenzi G,Rossi G,Vitale M

    更新日期:2009-05-01 00:00:00

  • HBP induces the expression of monocyte chemoattractant protein-1 via the FAK/PI3K/AKT and p38 MAPK/NF-κB pathways in vascular endothelial cells.

    abstract::Inflammation is characterized by early influx of polymorphonuclear neutrophils (PMNs), followed by a second wave of monocyte recruitment. PMNs mediate monocyte recruitment via their release of heparin binding protein (HBP), which activates CCR2 (CC-chemokine receptor 2) on monocytes. However, the pathways for such sig...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Chang M,Guo F,Zhou Z,Huang X,Yi L,Dou Y,Huan J

    更新日期:2018-03-01 00:00:00

  • Negative regulation of Jak2 by its auto-phosphorylation at tyrosine 913 via the Epo signaling pathway.

    abstract::Janus kinase 2 (Jak2) has a pivotal role in erythropoietin (Epo) signaling pathway, including erythrocyte differentiation and Stat5 activation. In the course of screening for critical phosphorylation of tyrosine residues in Jak2, we identified tyrosine 913 (Y(913)) as a novel and functional phosphorylation site, which...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Funakoshi-Tago M,Tago K,Kasahara T,Parganas E,Ihle JN

    更新日期:2008-11-01 00:00:00

  • A steroid alkaloid derivative 02F04 upregulates thymic stromal lymphopoietin expression slowly and continuously through a novel Gq/11-ROCK-ERK1/2 signaling pathway in mouse keratinocytes.

    abstract::Thymic stromal lymphopoietin (TSLP), a master switch of allergic inflammation, plays an important role in the pathogenesis of allergic diseases. Although many compounds upregulate TSLP expression in vivo or in vitro, most of them are pollutants or toxicants. In the previous study, for the first time, we found that a s...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Weng Y,Wang J,Yang Z,Xi M,Duan J,Guo C,Yin Y,Segawa R,Moriya T,Yonezawa T,Cha BY,Woo JT,Wen A,Hirasawa N

    更新日期:2019-05-01 00:00:00

  • The Na+, K(+)-ATPase inhibitor ouabain and the Na+ ionophore monensin have opposite effects upon carbachol-stimulated inositol phospholipid breakdown in rat cerebral cortical miniprisms.

    abstract::The effects of ouabain and monensin upon basal and carbachol-stimulated inositol phospholipid breakdown in rat cerebral cortical miniprisms have been investigated. Basal inositol phospholipid breakdown was increased by both compounds at both 6 and 18 mM K+. Enhancement of the carbachol response at 6 mM, but not at 18 ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Tiger G,Fowler CJ

    更新日期:1991-01-01 00:00:00

  • Synergistic effect of endothelin-1 and cyclic AMP on glucose transport in 3T3-L1 adipocytes.

    abstract::We have demonstrated previously that chronic exposure to endothlin-1 enhances glucose transport in 3T3-L1 adipocytes via augmented GLUT1 mRNA and protein accumulation. In the present study, we further examined the combined effect of endothelin-1 (ET-1) and cAMP on glucose transport. In cells pretreated with ET-1 and 8...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Fong JC,Kao YS,Tsai HY,Chiou YY,Chiou GY

    更新日期:2004-07-01 00:00:00

  • Negative regulation of interferon-γ/STAT1 signaling through cell adhesion and cell density-dependent STAT1 dephosphorylation.

    abstract::Signal transducer and activator of transcription 1 (STAT1) is an important mediator for cytokine signal transduction, particularly IFN-γ. Following IFN-γ stimulation, STAT1 is activated through tyrosine phosphorylation. Little is known about the function and regulation of STAT1 dephosphorylation after activation. We s...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Chen Z,Ma X,Zhang H,Sun X,Shen S,Li Y,Gu Y,Wang Y,Yan S,Yu Q

    更新日期:2011-08-01 00:00:00

  • Function of phosducin-like proteins in G protein signaling and chaperone-assisted protein folding.

    abstract::Members of the phosducin gene family were initially proposed to act as down-regulators of G protein signaling by binding G protein betagamma dimers (Gbetagamma) and inhibiting their ability to interact with G protein alpha subunits (Galpha) and effectors. However, recent findings have over-turned this hypothesis by sh...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Willardson BM,Howlett AC

    更新日期:2007-12-01 00:00:00

  • GATA3-driven expression of miR-503 inhibits prostate cancer progression by repressing ZNF217 expression.

    abstract::Although increasing evidence demonstrated that deregulation of mircoRNA-503 (miRNA-503) contributes to tumorigenesis, little is known about the biological role and intrinsic regulatory mechanisms of miR-503 in prostate cancer (PCa). In present study, we found that miR-503 was significantly downregulated in advanced PC...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Jiang X,Chen Y,Du E,Yang K,Zhang Z,Qi S,Xu Y

    更新日期:2016-09-01 00:00:00

  • Constitutive serum response factor activation by the viral chemokine receptor homologue pUS28 is differentially regulated by Galpha(q/11) and Galpha(16).

    abstract::Expression of the human cytomegalovirus (HCMV)-encoded chemokine receptor homologue pUS28 in mammalian cells results in ligand-dependent and -independent changes in the activity of multiple cellular signal transduction pathways. The ligand-dependent signalling activity of pUS28 has been shown to be predominantly media...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Moepps B,Tulone C,Kern C,Minisini R,Michels G,Vatter P,Wieland T,Gierschik P

    更新日期:2008-08-01 00:00:00

  • Different sensitivities of neutrophil responses to a selective protein kinase C inhibitor Ro 31-8425; redundancy in signal transduction.

    abstract::Previous studies implicating a role for protein kinase C (PKC) in mediating stimulation of cellular responses by physiological agonists have relied on use of non-specific inhibitors or direct stimulation of PKC by phorbol esters. However, much of this evidence is questionable. Here, we have investigated the effects of...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Merritt JE,Sullivan JA,Tse J,Wilkinson Se,Nixon JS

    更新日期:1997-01-01 00:00:00

  • Bradykinin-stimulated p42/p44 MAPK activation associated with cell proliferation in corneal keratocytes.

    abstract::Bradykinin (BK) is released into the tear-film in ocular allergic patients. BK has been shown to exert mitogenic effects on several cell types. However, the mechanisms underlying its action on corneal keratocytes (CKs) were largely unknown. This study was to investigate the mitogenic effect of BK on rabbit CKs linked ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Cheng CY,Huang SC,Hsiao LD,Sun CC,Jou MJ,Yang CM

    更新日期:2004-05-01 00:00:00

  • Tamoxifen blocks both proliferation and voltage-dependent K+ channels of neuroblastoma cells.

    abstract::The effects of tamoxifen (TAM) on cell proliferation and voltage-dependent K+ channels were studied on the mouse neuroblastoma cells NG 108-15. TAM inhibited cell proliferation with an effective dose inducing a half maximum effect (ED50) of 2 microM and was cytotoxic from and beyond 2.5 microM. TAM accelerated the app...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Rouzaire-Dubois B,Dubois JM

    更新日期:1990-01-01 00:00:00

  • Transforming growth factor beta 1 potently activates CPP32-like proteases in human hepatoma cells.

    abstract::Induction of apoptosis in Hep3B hepatoma cells by transforming growth factor beta 1 (TGF-beta 1) was accompanied by the activation of interleukin-1-beta-converting-enzyme-like proteases, which have recently been renamed as caspases. The caspase inhibitor ZVAD-FMK, which has a broader specificity for caspase family pro...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Hung WC,Chang HC,Chuang LY

    更新日期:1998-07-01 00:00:00

  • Bacterial ligand of TLR2 signals Stat activation via induction of IRF1/2 and interferon-alpha production.

    abstract::Both type I interferons (IFNs) and interferon regulatory factors (IRFs) are well characterized in viral infections, whereas they are far less studied in bacterially activated toll-like receptor (TLR) pathways. Here, we studied the involvement of IRF1 and IRF2 in TLR2-mediated responses. In mouse macrophages, IRF2 was ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Liljeroos M,Vuolteenaho R,Rounioja S,Henriques-Normark B,Hallman M,Ojaniemi M

    更新日期:2008-10-01 00:00:00

  • Refining the minimal sequence required for ERK1/2-dependent poly-ubiquitination and proteasome-dependent turnover of BIM.

    abstract::The pro-apoptotic protein BIM(EL) is phosphorylated by ERK1/2 and this targets the protein for poly-ubiquitination and degradation by the proteasome as a survival mechanism. To define in greater detail the sequence determinants required for BIM(EL) turnover we have compared various BIM splice variants and truncation m...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Wiggins CM,Johnson M,Cook SJ

    更新日期:2010-05-01 00:00:00