Abstract：
BACKGROUND:Although Leishmania virulence may be modulated by environmental and genetic factors of their mammalian hosts and sand fly vectors, molecular determinants of Leishmania sp. are the key elements. This work evidences that Leishmania amazonensis axenic amastigotes produce comparatively more NO than infective promastigotes. METHODS:A soluble NOS was purified from L. amazonensis axenic amastigotes by affinity chromatography (2',5'-ADP-agarose), and on SDS-PAGE the enzyme migrates as a single protein band. RESULTS:The presence of a constitutive NOS was detected through immunofluorescence using antibody against neuronal NOS (nNOS) and in NADPH consumption assays. CONCLUSIONS:The present data show that NOS is prominent in axenic amastigote preparations, suggesting an association with the infectivity and/or an escaping mechanism of the parasite. The relationship between the NO-generating systems in the parasite and in their host cell warrants further investigation.
journal_name
Arch Med Resjournal_title
Archives of medical researchauthors
Genestra M,Guedes-Silva D,Souza WJ,Cysne-Finkelstein L,Soares-Bezerra RJ,Monteiro FP,Leon LLdoi
10.1016/j.arcmed.2005.07.011subject
Has Abstractpub_date
2006-04-01 00:00:00pages
328-33issue
3eissn
0188-4409issn
1873-5487pii
S0188-4409(05)00311-5journal_volume
37pub_type
杂志文章abstract：BACKGROUND:A recent revelation about increased susceptibility to HIV by use of nonoxynol-9 (N-9) has called for identification of novel molecules with potent sperm-attenuating activity and lower side-effect profile, as suitable alternatives. The present study was designed to investigate spermicidal activity in Bohadsch...
journal_title：Archives of medical research
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abstract：BACKGROUND AND AIMS:In previous studies, the Pro12Ala polymorphism in the peroxisome proliferator-activated receptor gamma 2 (PPAR-γ2) was shown to be associated with both lipid metabolism and longevity. We examined whether the polymorphism continued to be associated with abnormal levels of serum lipid/lipoprotein amon...
journal_title：Archives of medical research
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journal_title：Archives of medical research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0188-4409(01)00315-0
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abstract：:Hepatitis C represents more than 35% of liver transplant candidates worldwide. Meanwhile, hepatitis B continues to be an important cause of end-stage liver disease and hepatocellular carcinoma in Asia and Africa. Recurrent viral liver disease is a significant event after liver transplantation and continues to be one o...
journal_title：Archives of medical research
pub_type： 杂志文章,评审
doi：10.1016/j.arcmed.2006.09.003
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abstract：BACKGROUND:Associations between hyperuricemia, metabolic syndrome, cardiovascular disease and diabetes have been reported. Limited information, however, is available concerning the prevalence and correlates of hyperuricemia among Thai men and women. We sought to estimate the prevalence of hyperuricemia among a populati...
journal_title：Archives of medical research
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abstract：BACKGROUND AND AIMS:Jagged1/Notch3 signaling pathway plays a key role in angiogenesis of breast cancer, but little is known in TNBC. This study was designed to investigate the expression of Jagged1/Notch3 mRNA and protein in TNBC, analyze their correlations with clinicopathological characteristics and prognosis. Moreov...
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doi：
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abstract：BACKGROUND:Patients with certain types of stroke need urgent anticoagulation and it is extremely important for them to achieve fast and stable anticoagulant effect and receive individualized treatment during the initiation of warfarin therapy. METHODS:We conducted a prospective study among 210 acute stroke patients wh...
journal_title：Archives of medical research
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journal_title：Archives of medical research
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journal_title：Archives of medical research
pub_type： 杂志文章,评审
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journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2007.02.005
更新日期：2007-07-01 00:00:00
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journal_title：Archives of medical research
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
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journal_title：Archives of medical research
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doi：
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journal_title：Archives of medical research
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abstract：:The identification of human IgG immunoglobulins on the surface of Vibrio cholerae O1, and Haemophilus influenzae type b microorganisms was assessed via a flow cytometric technique. A group of 31 healthy mother-full term newborn duo sera from a non-endemic cholera area was assayed. The sera of mothers and full-term new...
journal_title：Archives of medical research
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doi：
更新日期：1996-04-01 00:00:00
abstract：:The fundamental principle of stem cell biology is that cells with the potential for both self-renewal and terminal differentiation into one or more cell types may be found in a given tissue. The corollary of this principle is that the stem cells give rise to tissues in which they reside, the so-called expected tissues...
journal_title：Archives of medical research
pub_type： 杂志文章,评审
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journal_title：Archives of medical research
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doi：
更新日期：1997-04-01 00:00:00
abstract：BACKGROUND:This study investigated the association of working conditions and lifestyle with the development of increased serum gamma-glutamyltransferase (GGT) in Japanese workers. METHODS:A follow-up study was carried out on workers of a telecommunication enterprise who received their first annual health check-up betw...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2005.03.027
更新日期：2005-09-01 00:00:00
abstract：:During the process of energy production in aerobic respiration, vascular cells produce reactive oxygen species (ROS). A growing body of evidence indicates that oxidative stress refers to a condition in which cells are subjected to excessive levels of ROS. Overall vascular function is dependent upon a fine balance of o...
journal_title：Archives of medical research
pub_type： 杂志文章,评审
doi：10.1016/j.arcmed.2005.11.012
更新日期：2006-05-01 00:00:00
abstract：BACKGROUNDS:Rheumatoid arthritis (RA) is a systemic autoimmune disease that confers one of the strongest risks for cardiovascular disease (CVD) morbidity and mortality than in general population. Pulse wave velocity (PWV) and augmentation index (AIx) are composite measures of arterial stiffness (AS) and associated with...
journal_title：Archives of medical research
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doi：10.1016/j.arcmed.2019.10.010
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abstract：:As an initial step of investigation into the regulatory mechanisms of encystation in Entamoeba, we compared the protein profiles of newly formed cysts and trophozoites of E. invadens using high resolution two-dimensional PAGE and digitized video image analysis of silver stained gels. A total of 155 proteins unique to ...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND AND AIMS:To compare the prevalence of lactose malabsorption (LM) in children by analyzing hydrogen content in expired air vs. the inclusion of methane excretion. METHODS:A total of 138 children (4-17 years old) attending different boarding schools in Mexico were included in this study. To measure H₂ and CH₄...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2013.04.005
更新日期：2013-05-01 00:00:00
