Abstract:
:An efficient delivery system is required if antisense oligodeoxynucleotides (ODN) are to be utilized for gene therapy. We report herein on the development of a novel ODN delivery system, ODN-encapsulated nano particles (ODN-ENP) using an efficient and simple packaging method. The ODN-ENP consists of a condensed ODN particle and a lipid envelope, which can be equipped with various functional devices for the efficient delivery of ODN with a small diameter (150 nm). The encapsulation efficiency and ODN recovery of ODN-ENP were significantly higher than those of other packaging methods, such as a stabilized antisense-lipid particles method or a freeze-thaw method. Furthermore, the time required for the preparation of the ODN-ENP was shorter than the other methods. The method developed in this study is a simple and efficient packaging method for ODN with a condensed nano particle in lipid-envelope structure.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Yamada Y,Kogure K,Nakamura Y,Inoue K,Akita H,Nagatsugi F,Sasaki S,Suhara T,Harashima Hdoi
10.1248/bpb.28.1939subject
Has Abstractpub_date
2005-10-01 00:00:00pages
1939-42issue
10eissn
0918-6158issn
1347-5215pii
JST.JSTAGE/bpb/28.1939journal_volume
28pub_type
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