Abstract:
:The estimation of metallothionein (MT) levels in a biological sample is required to study the physiological role or the induction of MT, which is a multifunctional protein. MT contains 61 amino acids with 20 homologous cysteines. The high selectivity, sensitivity and stability of N-[4-(dimethylamino-2-benzofuranyl)phenyl]maleimide (DBPM) for the determination of SH groups was very useful for the determination of MT. The detection limit of MT by this method was 10 ng/0.5 ml. This method made it possible to measure MT levels in normal tissues in combination with gel filtration chromatography. The age-dependent change in MT levels in normal rat tissues was also investigated by the present method. MT levels in normal rat tissues vary with age. It is thought that this fact is correlated with the biological roles of MT.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Shinogi M,Nishinaga K,Yokoyama Idoi
10.1248/bpb.19.911subject
Has Abstractpub_date
1996-07-01 00:00:00pages
911-4issue
7eissn
0918-6158issn
1347-5215journal_volume
19pub_type
杂志文章abstract::We performed experiments on the cis-inhibition and trans-stimulation effect on pirarubicin uptake in order to clarify the involvement of a carrier in the pirarubicin, daunorubicin and/or doxorubicin transport systems in rat polymorphonuclear leukocytes. The uptake of daunorubicin and doxorubicin was a saturable concen...
journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1248/bpb.18.1694
更新日期:1995-12-01 00:00:00
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更新日期:2000-04-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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journal_title:Biological & pharmaceutical bulletin
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更新日期:1996-02-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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journal_title:Biological & pharmaceutical bulletin
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更新日期:2005-08-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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journal_title:Biological & pharmaceutical bulletin
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journal_title:Biological & pharmaceutical bulletin
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章,评审
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更新日期:2012-01-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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更新日期:2017-01-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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更新日期:1996-04-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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journal_title:Biological & pharmaceutical bulletin
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journal_title:Biological & pharmaceutical bulletin
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更新日期:1995-11-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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journal_title:Biological & pharmaceutical bulletin
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:1995-05-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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journal_title:Biological & pharmaceutical bulletin
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更新日期:2018-12-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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