Mitogenic activity and the induction of tumor necrosis factor by lipopeptide analogs of the N-terminal part of lipoprotein in the outer membrane of Escherichia coli.


:Mitogenicity and the production of tumor necrosis factor (TNF) by a chemically synthesized lipotetrapeptide analog (KAB-8), S-[2,3-bis(palmitoyloxy)-2R-propyl]-N-[(2,2,2)-trichloro- ethoxycarbonyl: Troc group]-(R)-cysteinyl-(S)-seryl-(S)-seryl-(S)-asparagine, the amino acid sequence of which corresponds to that of the lipopeptide part of lipoprotein in Escherichia coli, and several derivatives (KAB-30-41), which possessed the altered glycerocysteine moiety, were examined. A 1-cysteinyl glycerol skeleton-type compound (KAB-8), a propane-type compound (KAB-31), a homoglycerol-type (KAB-39 and 40) and a 2-cysteinyl glycerol-type (KAB-41) exhibited mitogenic activity on splenocytes from C3H/He mice at various concentrations ranging from 0.4 to 100 micrograms/ml. However, propane-type compounds, except KAB-31, and ethane-type compounds showed lower mitogenic activity than other types of compounds. Compounds KAB-8, 31, 40 and 41 induced the production of TNF in peritoneal exudated macrophages from C3H/He mice at concentrations of 25 and 50 micrograms/ml. The results indicate that the structural differences of the glycerol moiety in the synthetic lipopeptides affect the potency of its biological activities.


Biol Pharm Bull


Shimizu T,Iwamoto Y,Yanagihara Y,Kurimura M,Achiwa K




Has Abstract


1994-07-01 00:00:00












  • Characterization of partially purified alpha-glucosidase in the insoluble fraction of bovine crystalline lens.

    abstract::Two fractions of neutral alpha-glucosidase were partially purified from the insoluble fraction of bovine lens. This is the first report of such an event to the best of our knowledge. The apparent native molecular weights of these fractions were 121 kDa (fraction-I) and 254 kDa (fraction-II). Both fractions contained t...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kamei A,Fujiyama O

    更新日期:1995-08-01 00:00:00

  • A Novel Bongkrekic Acid Analog-Mediated Modulation of the Size of Lipid Droplets: Evidence for the Appearance of Smaller Adipocytes.

    abstract::Thiazolidinediones (TZDs) are known as peroxisome proliferator-activated receptor γ (PPARγ) activators, and are used in the treatment of diabetes. Although the usefulness of TZDs has been demonstrated, some of their side effects are becoming an obstacle to their clinical applicability; edema is known to be evoked by t...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Okazaki H,Takeda S,Ishii H,Takemoto Y,Fujita S,Suyama M,Matsumoto K,Shindo M,Aramaki H

    更新日期:2017-01-01 00:00:00

  • Chrysin Inhibits Lymphangiogenesis in Vitro.

    abstract::The induction of lymphangiogenesis is an important process to promote cancer growth and cancer metastasis via the lymphatic system. Identifying the compounds that can prevent lymphangiogenesis for cancer therapy is urgently required. Chrysin, 5,7-dihydroxyflavone, a natural flavone extracted from Thai propolis, was us...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Prangsaengtong O,Athikomkulchai S,Xu J,Koizumi K,Inujima A,Shibahara N,Shimada Y,Tadtong S,Awale S

    更新日期:2016-01-01 00:00:00

  • Immunomodulatory effect of Astragali Radix extract on murine TH1/TH2 cell lineage development.

    abstract::Astragali Radix (AR), is a popular herbal medicine used to treat allergic diseases in Korea, Japan and China. Our study examined the effect of an AR ethanol extract on both in vitro and in vivo murine CD4 T cells' differentiation into Th1 and Th2 subsets. CD4 T cells from Balb/c mice were activated with anti-CD3/anti-...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kang H,Ahn KS,Cho C,Bae HS

    更新日期:2004-12-01 00:00:00

  • Cytochalasin H, an active anti-angiogenic constituent of the ethanol extract of Gleditsia sinensis thorns.

    abstract::Angiogenesis, the process of new vessel formation from the pre-existing blood vasculature, is critical for continuous tumor growth and is considered to be a validated antitumor target. The results of our previous study demonstrate the anti-angiogenic potential of an extract of Gleditsia sinensis thorns, which has been...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Lee J,Yi JM,Kim H,Lee YJ,Park JS,Bang OS,Kim NS

    更新日期:2014-01-01 00:00:00

  • Gastric emptying-limited oral absorption of alpha-linolenic acid administered as a milk fat-globule membrane (MFGM) emulsion in rats.

    abstract::As a study to assess the potential application of milk fat-globule membrane (MFGM), which is of natural origin and expected to be a safer alternative to synthetic emulsifiers, to pharmaceutical dosage forms, the oral absorption of alpha-linolenic acid was evaluated by the analysis of gastrointestinal disposition after...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yuasa H,Sekiya M,Ozeki S,Watanabe J

    更新日期:1994-09-01 00:00:00

  • Antioxidant Activity of Ge-132, a Synthetic Organic Germanium, on Cultured Mammalian Cells.

    abstract::Ge-132 is a synthetic organic germanium that is used as a dietary supplement. The antioxidant activity of Ge-132 on cultured mammalian cells was investigated in this study. First, Ge-132 cytotoxicity on mammalian cultured cells was determined by measuring lactate dehydrogenase (LDH) levels. Ge-132 had no cytotoxic eff...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Wada T,Hanyu T,Nozaki K,Kataoka K,Kawatani T,Asahi T,Sawamura N

    更新日期:2018-05-01 00:00:00

  • Expression and characterization of human rheumatoid factor single-chain Fv.

    abstract::The variable region of heavy chain [V(H)] of human rheumatoid factor (hRF) IgM was connected with the variable region of light chain [V(L)] with the peptide-linker (GGGSGGGSGGGS) by genetic engineering method and the single-chain Fv (scFv) was expressed in E. coli. On design, scFv and scFv (tag) were planned; the latt...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Hashimoto Y,Ikenaga T,Tanigawa K,Ueda T,Ezak I,Imoto T

    更新日期:2000-08-01 00:00:00

  • Impacts of diversification of cytochrome P450 on plant metabolism.

    abstract::Cytochrome P450 monooxygenases (P450s) catalyze a wide variety of monooxygenation reactions in primary and secondary metabolism in plants. The share of P450 genes in each plant genome is estimated to be up to 1%. This implies that the diversification of P450 has made a significant contribution to the ability to acquir...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章,评审


    authors: Mizutani M

    更新日期:2012-01-01 00:00:00

  • Inhibitory effects of a nitric oxide synthase inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME), on 2-deoxy-D-glucose-induced hyperglycemia in rats.

    abstract::The inhibitory effects of a potent nitric oxide (NO) synthase inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME), on 2-deoxy-D-glucose (2-DG)-induced hyperglycemia were investigated in rats. L-NAME significantly inhibited 2-DG-induced hyperglycemia, although N(G)-nitro-D-arginine methyl ester (D-NAME) did not affe...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Sugimoto Y,Yamada J,Yoshikawa T,Horisaka K

    更新日期:1997-12-01 00:00:00

  • Protective effects of tea melanin against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced toxicity: antioxidant activity and aryl hydrocarbon receptor suppressive effect.

    abstract::We examined the protective ability of tea melanin against 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced toxicity in C57BL6J mice. Reduced tea melanin (RTM) and non-reduced tea melanin (NRTM) were incorporated to distinguish anti-oxidant activity from alternative pathways. The mice were given a single oral dose of...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Hung YC,Huang GS,Sava VM,Blagodarsky VA,Hong MY

    更新日期:2006-11-01 00:00:00

  • Purification and characterization of ginsenoside Ra-hydrolyzing beta-D-xylosidase from Bifidobacterium breve K-110, a human intestinal anaerobic bacterium.

    abstract::Beta-D-Xylosidase (EC has been purified from ginsenoside Ra-metabolizing Bifidobacterium breve K-110, which was isolated from human intestinal microflora. beta-D-Xylosidase was purified to apparent homogeneity by a combination of ammonium sulfate precipitation, QAE-cellulose, butyl-toyopearl, hydroxyapatit a...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Shin HY,Lee JH,Lee JY,Han YO,Han MJ,Kim DH

    更新日期:2003-08-01 00:00:00

  • Ninety-Day Subchronic Oral Toxicity Study of Senecio scandens Extract in Rats.

    abstract::The present study assessed the safety/toxicity of Senecio scandens, a well-known Chinese herb that is used as an anti-inflammatory, antibiosis, and antipyretic drug. A 90-d subchronic oral toxicity study of S. scandens was performed in Wistar rats. The extract of S. scandens was administered orally to male and female ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Wang XK,Zhao Y,Liu T,Yi Y,Li CY,Wang HJ,Wang CH,Wang ZT,Ye ZG,Liang AH

    更新日期:2015-01-01 00:00:00

  • Apoptosis inducing activity of fucoidan in HCT-15 colon carcinoma cells.

    abstract::The antitumor activity of fucoidan from Fucus vesiculosus was investigated in human colon carcinoma cells. The crude fucoidan, a polysaccharide composed predominantly of sulfated fucose, markedly inhibited the growth of HCT-15 cells (human colon carcinoma cells). After HCT-15 cells were treated with fucoidan, several ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Hyun JH,Kim SC,Kang JI,Kim MK,Boo HJ,Kwon JM,Koh YS,Hyun JW,Park DB,Yoo ES,Kang HK

    更新日期:2009-10-01 00:00:00

  • Properties and human origin of two angiotensin-I-converting enzyme inhibitory peptides isolated from a tryptic hydrolysate of human serum albumin.

    abstract::Two angiotensin-I-converting enzyme (ACE) inhibitory peptides were isolated from a tryptic hydrolysate of human serum albumin (HSA). The peptides were identified by sequencing and other analyses as Ala-Trp and the nonapeptide Ala-Phe-Lys-Ala-Trp-Ala-Val-Ala-Arg (human albutensin A), corresponding to f(213-214) and f(2...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Nakagomi K,Ebisu H,Sadakane Y,Fujii N,Akizawa T,Tanimura T

    更新日期:2000-07-01 00:00:00

  • Albumin-Based Nitric Oxide Traffic System for the Treatment of Intractable Cancers.

    abstract::Biomacromolecules (>40 kDa) have been developed as drug delivery system (DDS) carriers of low-molecular weight drugs to promote these drugs' uptake by cancer tissues via enhanced permeability and retention (EPR) effects. Human serum albumin (HSA) has been found to accumulate in cancer tissues via this EPR effect. HSA ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章,评审


    authors: Ishima Y

    更新日期:2017-01-01 00:00:00

  • Effects of β-Naphthoflavone on Ugt1a6 and Ugt1a7 Expression in Rat Brain.

    abstract::Uridine 5'-diphosphate-glucuronosyltransferase (UGT) catalyzes a major phase II reaction in a drug-metabolizing enzyme system. Although the UGT1A subfamily is expressed mainly in the liver, it is also expressed in the brain. The purpose of the present study was to elucidate the effect of β-naphthoflavone (BNF), one of...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Sakakibara Y,Katoh M,Kondo Y,Nadai M

    更新日期:2016-01-01 00:00:00

  • Pharmacological characteristics of Ryokan-kyomi-shinge-nin-to, an antiallergic Kampo medicine.

    abstract::The pharmacological characteristics of Ryokan-kyomi-shinge-nin-to (RKS), a traditional oriental herbal (Kampo) medicine which has been used for the treatment of allergic asthma and rhinitis, were investigated. The number of sneezes by actively sensitized mice after a topical antigen challenge was significantly reduced...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Sakaguchi M,Ikeda Y,Kido T,Yuzurihara M,Kase Y,Yamamoto M,Ishige A,Sasaki H

    更新日期:2002-12-01 00:00:00

  • Inhibition of double-stranded RNA-induced inducible nitric oxide synthase expression by fraxinellone and sauchinone in murine microglia.

    abstract::Fraxinellone and sauchinone, isolated from natural substance, are known to have an anti-inflammatory effect in inflammatory conditions. However, the anti-inflammatory actions of these compounds have been insufficiently demonstrated in viral-induced neuroinflammation. A viral component (double-stranded (ds)RNA) trigger...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Lee CS,Won C,Yoo H,Yi EH,Cho Y,Maeng JW,Sung SH,Ye SK,Chung MH

    更新日期:2009-11-01 00:00:00

  • Reductive metabolism of stilbene oxide and styrene oxide to the olefins in rats.

    abstract::The present study provides the first evidence that stilbene oxide and styrene oxide are reductively metabolized to the corresponding olefins in rats. When cis- or trans-stilbene oxide was given orally to rats, both cis- and trans-stilbene were isolated from the urine and feces. Styrene was also isolated from the urine...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kitamura S,Shiraishi Y,Mita M,Sueki H,Maeda C,Tatsumi K

    更新日期:2000-02-01 00:00:00

  • Involvement of Ca2+/calmodulin-dependent protein kinase II in heparin-stimulated release of hepatic lipase activity from rat hepatocytes.

    abstract::The release of hepatic lipase (HTGL), which is responsible for the hydrolysis of lipoprotein triacylglyceride, produced by heparin from the isolated rat hepatocytes in primary culture has been examined. Tyrosine kinase (TK) inhibitors (ST-638 and biochanin A) inhibited the heparin-stimulated release of HTGL activity. ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Tagashira H,Nakahigashi S,Kerakawati R,Motoyashiki T,Morita T

    更新日期:2005-03-01 00:00:00

  • Low Viability of Cholera Toxin-Producing Vibrio cholerae O1 in the Artificial Low Ionic Strength Aquatic Solution.

    abstract::It has been well known that Vibrio cholerae inhabit in environmental water. As many patients infected with cholera toxin-producing V. cholerae O1 (toxigenic V. cholerae O1) emerge in Kolkata, India, it has been thought that toxigenic V. cholerae O1 is easily detected in environmental water in Kolkata. However, we coul...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Paul SS,Takahashi E,Chowdhury G,Miyoshi SI,Mizuno T,Mukhopadhyay AK,Dutta S,Okamoto K

    更新日期:2020-08-01 00:00:00

  • Extract from Nandina domestica inhibits lipopolysaccharide-induced cyclooxygenase-2 expression in human pulmonary epithelial A549 cells.

    abstract::Extract from fruits of Nandina domestica THUNBERG (NDE) has been used to improve cough and breathing difficulty in Japan for many years. To explore whether NDE may alleviate respiratory inflammation, we investigated its effect on expression of cyclooxygenase-2 (COX-2) and production of prostaglandin E₂ (PGE₂) in human...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ueki T,Akaishi T,Okumura H,Abe K

    更新日期:2012-01-01 00:00:00

  • Investigation of the safety of topical metronidazole from a pharmacokinetic perspective.

    abstract::Metronidazole (MTZ) ointment has been used widely as a hospital preparation against cancerous malodor. Although cancerous tissue with ulcer-like symptoms is likely to have a higher capacity to absorb drugs than normal skin, the extent to which MTZ is absorbed when a topical preparation is applied to cancerous tissue r...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Iida J,Kudo T,Shimada K,Yatsuno Y,Yamagishi S,Hasegawa S,Ike H,Sato T,Kagaya H,Ito K

    更新日期:2013-01-01 00:00:00

  • Macelignan attenuates activations of mitogen-activated protein kinases and nuclear factor kappa B induced by lipopolysaccharide in microglial cells.

    abstract::A previous study showed that macelignan extracted from Myristica fragrans has anti-inflammatory properties using hippocampal neuronal and primary microglial cells. Subsequently, a study using animals with chronic lipopolysaccharide (LPS) infusion into the brain showed that oral treatments of macelignan reduced the hip...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ma J,Hwang YK,Cho WH,Han SH,Hwang JK,Han JS

    更新日期:2009-06-01 00:00:00

  • Inhibitory effects of 5-S-GAD on phosphorylation of V-SRC and BCR-ABL tyrosine kinase.

    abstract::We examined the inhibitory effects of N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), a novel antibacterial substance from the immunized adult Sarcophaga peregrina (flesh fly), on protein phosphorylation using immune complexes of protein tyrosine kinases (PTKs) with anti-PTKs monoclonal antibody. ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Leem JY,Park HY,Fukazawa H,Uehara Y,Natori S

    更新日期:1998-07-01 00:00:00

  • Antinociceptive activities of 70% methanol extract of evodiae fructus (fruit of Evodia rutaecarpa var. bodinieri) and its alkaloidal components.

    abstract::The effects of 70% methanol extract (EA-ext) from Evodiae Fructus (EA) consisting of dried fruits of Evodia rutaecarpa var. bodinieri (Rutaceae) on nociceptive responses were investigated. Oral administration of 50 or 200 mg/kg EA-ext had the same antinociceptive effect on writhing responses as induced by acetic acid....

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Matsuda H,Wu JX,Tanaka T,Iinuma M,Kubo M

    更新日期:1997-03-01 00:00:00

  • Lower Squalene Epoxidase and Higher Scavenger Receptor Class B Type 1 Protein Levels Are Involved in Reduced Serum Cholesterol Levels in Stroke-Prone Spontaneously Hypertensive Rats.

    abstract::A lower serum cholesterol level was recently shown to be one of the causes of stroke in an epidemiological study. Spontaneously hypertensive rats stroke-prone (SHRSP) have lower serum cholesterol levels than normotensive Wistar-Kyoto rats (WKY). To elucidate the mechanisms responsible for the lower serum cholesterol l...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Michihara A,Mido M,Matsuoka H,Mizutani Y

    更新日期:2015-01-01 00:00:00

  • Advances in CRISPR/Cas9 Technology for in Vivo Translation.

    abstract::Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology has revolutionized therapeutic gene editing by providing researchers with a new method to study and cure diseases previously considered untreatable. While the full range and power of CRISPR technology for t...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章,评审


    authors: Çiçek YA,Luther DC,Kretzmann JA,Rotello VM

    更新日期:2019-01-01 00:00:00

  • Tissue-specific mRNA expression profiles of human carbohydrate sulfotransferase and tyrosylprotein sulfotransferase.

    abstract::Pairs of forward and reverse primers and TaqMan probes specific to each of 15 human sulfotransferases were prepared. The mRNA expression level of each target enzyme was analyzed in total RNA from single and pooled specimens of various human tissues (adrenal gland, bone marrow, brain, colon, heart, kidney, liver, lung,...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Nishimura M,Naito S

    更新日期:2007-04-01 00:00:00