Real time measurement of nitric oxide released from cultured endothelial cells.

abstract：:The effect of fungichromin (FC) on the formation of Candida albicans biofilm was assessed using 2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction method, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Results showed that FC revealed an inhibitor...

journal_title：Biological & pharmaceutical bulletin

authors： An D,Wang X,Li J,Jiang S,Ma X,Zhang H,Shi H,Sun H,Ye L,Li J

更新日期：2016-12-01 00:00:00

abstract：:Inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) play a key role in the inflammatory processes. Improper overproduction of NO and prostaglandins by both enzymes are also believed to be involved in the pathogenesis of certain human cancers. Crude extracts of Selaginella tamariscina are used as an ori...

journal_title：Biological & pharmaceutical bulletin

authors： Woo ER,Pokharel YR,Yang JW,Lee SY,Kang KW

更新日期：2006-05-01 00:00:00

abstract：:We investigated a method for the simultaneous screening, identification, and quantitative determination of salicylic acid, acetaminophen, theophylline, barbiturates, and bromvalerylurea, drugs that frequently cause acute poisoning in Japan and therefore require rapid analysis for effective treatment in the clinical se...

journal_title：Biological & pharmaceutical bulletin

authors： Hori Y,Fujisawa M,Shimada K,Hirose Y,Yoshioka T

更新日期：2006-01-01 00:00:00

abstract：:The weights of prostate and seminal vesicle were decreased by treatment with 3,6-dimethylpyrazine-2-thiol(3,6-DMP-SH) but the weights of testes and the plasma testosterone levels were not changed. The recovery of the weight of prostate and seminal vesicles of testosterone-treated orchidectomized rats were inhibited by...

journal_title：Biological & pharmaceutical bulletin

authors： Yamada K,Komatsu H,Ohta A

更新日期：1996-03-01 00:00:00

abstract：:A traditional Chinese herbal medicine, Qizhu Tang (QZT) was studied for its in vitro antioxidant activity and the effect on cerebral oxidative damage after forebrain ischemia followed by reperfusion in rats. The QZT decoction was shown to have strong hydroxyl radical (*OH) scavenging activity (approx. 0.1 mM as Trolox...

journal_title：Biological & pharmaceutical bulletin

authors： Xuejiang W,Ichikawa H,Konishi T

更新日期：2001-05-01 00:00:00

abstract：:Corneal transplantation has been used to treat severe eye disease for decades, but the therapeutic effect of the operation is highly compromised by immunological allograft rejection. To improve the success rate of corneal transplantation, we studied the protective effects of cyclosporine nanomicelle eye drops (CNED) o...

journal_title：Biological & pharmaceutical bulletin

authors： Zhang H,Wang L,Zhang L

更新日期：2015-01-01 00:00:00

abstract：:Two known diarylheptanoids, oregonin (1), (5S)-1,7-bis-(3,4-dihydroxyphenyl)-heptane-3-one-5-O-beta-D-xylopyranosi de and hirsutanonol (2), (5S)-1,7-bis-(3,4-dihydroxyphenyl)-5-hydroxyheptane-3-one isolated from the bark of Alnus hirsuta var. sibirica, showed significant inhibitory effects on 12-O-tetradecanoylphorbol...

journal_title：Biological & pharmaceutical bulletin

authors： Lee MW,Kim JH,Jeong DW,Ahn KH,Toh SH,Surh YJ

更新日期：2000-04-01 00:00:00

abstract：:We examined the transport mechanisms of daunorubicin (DNR) and doxorubicin (ADR) in HL60 and HL60/THP cells which were the non-P-glycoprotein-mediated resistant clone of the parent HL60 cells and showed a low degree of resistance, and compared them with those of pirarubicin (THP). In both lines, it appeared that the u...

journal_title：Biological & pharmaceutical bulletin

authors： Nagasawa K,Natazuka T,Nomiyama M,Ohnishi N,Yokoyama T

更新日期：1996-01-01 00:00:00

abstract：:Nitric oxide (NO) and the partial pressure of oxygen (pO(2)) in the liver were simultaneously quantified in rats with partial hepatic ischemia/reperfusion injury (PHIRI). Real-time NO/pO(2) monitoring and immunohistochemical analysis for superoxide dismutase and inducible nitric oxide synthase (iNOS) and endothelial N...

journal_title：Biological & pharmaceutical bulletin

authors： Hataji K,Watanabe T,Oowada S,Nagaya M,Kamibayashi M,Murakami E,Kawakami H,Ishiuchi A,Kumai T,Nakano H,Kobayashi S,Otsubo T

更新日期：2010-01-01 00:00:00

abstract：:The variable region of heavy chain [V(H)] of human rheumatoid factor (hRF) IgM was connected with the variable region of light chain [V(L)] with the peptide-linker (GGGSGGGSGGGS) by genetic engineering method and the single-chain Fv (scFv) was expressed in E. coli. On design, scFv and scFv (tag) were planned; the latt...

journal_title：Biological & pharmaceutical bulletin

authors： Hashimoto Y,Ikenaga T,Tanigawa K,Ueda T,Ezak I,Imoto T

更新日期：2000-08-01 00:00:00

abstract：:6-(10-Hydroxydecyl)-2,3-dimethoxy-5-methyl-1,4-benzoquinone (idebenone) has been found to have a membrane-stabilizing activity in studies using lysosomes. Using dimyristoyl L-alpha-phosphatidylcholine (DMPC) as a substrate, the effects of idebenone and related benzoquinones on phospholipid digestion by phospholipase A...

journal_title：Biological & pharmaceutical bulletin

authors： Amano T,Terao S,Imada I

更新日期：1995-05-01 00:00:00

abstract：:In the present study, we evaluated the effect of the novel acid pump antagonist 7-(4-fluorobenzyloxy)-2,3-dimethyl-1-{[(1S,2S)-2-methylcyclopropyl]methyl}-1H-pyrrolo[2,3-d]pyridazine (CS-526) on the intragastric acidity of cynomolgus monkeys. The study was performed in a crossover manner with five male animals. CS-526...

journal_title：Biological & pharmaceutical bulletin

authors： Ito K,Kinoshita K,Yamamura N,Tomizawa A,Inaba F,Morikawa-Inomata Y,Tabata K,Shibakawa N

更新日期：2009-12-01 00:00:00

abstract：:An increase in the number of glucocorticoid-induced tumor necrosis factor receptor-family related gene/protein (GITR)+CD25- (or fork-head box protein 3: Foxp3-) CD4+ T cells, after treating a mouse model of arthritis with fingolimod (FTY720), and a pathogenic antigen may play a key role in the establishment of immune ...

journal_title：Biological & pharmaceutical bulletin

authors： Yoshida Y,Mikami N,Nakanishi Y,Saimoto M,Nagaike A,Shimono H,Nakano S,Tsuji T,Kohno T

更新日期：2021-01-26 00:00:00

abstract：:This study investigated both morphologically and biochemically whether parsley (Petroselinum crispum), which is used as a folk remedy to decrease blood glucose, has any antidiabetic effect on pancreatic B cells of rats. Parsley extract was given to male diabetic rats. In the diabetic group given parsley extract, it wa...

journal_title：Biological & pharmaceutical bulletin

authors： Yanardağ R,Bolkent S,Tabakoğlu-Oğuz A,Ozsoy-Saçan O

更新日期：2003-08-01 00:00:00

abstract：:The inhibitory effects of tetrandrine (an alkaloid isolated from the Chinese medicine Stephania tetrandrae S. Moore) were investigated in terms of the angiogenesis in an adjuvant-induced chronic inflammation model of mouse and tube formation of rat vascular endothelial cells (EC). Tetrandrine (7.5-30 mg/kg) reduced th...

journal_title：Biological & pharmaceutical bulletin

authors： Kobayashi S,Inaba K,Kimura I,Kimura M

更新日期：1998-04-01 00:00:00

abstract：:The objective of this study was to evaluate the interactions between various drugs and aojiru (green juice), a popular health food in Japan, using a simple centrifugation method. The mixture of drug solution and aojiru suspension was gently shaken and centrifuged. The drug concentration in the supernatant fluid was th...

journal_title：Biological & pharmaceutical bulletin

authors： Iwao K,Kawai RT,Oda M,Saitoh H

更新日期：2017-01-01 00:00:00

abstract：:Apoptosis is the main form of β-cell death in diabetes. Ginseng has been used as an anti-diabetic herb for several thousand years in Asia with ginsenoside Rg1 and ginsenoside Rb1 as important active ingredients. In this study, we demonstrated ginsenoside Rg1 and Rb1 protect β-cells from high glucose/cytokine-induced p...

journal_title：Biological & pharmaceutical bulletin

authors： Chen F,Chen Y,Kang X,Zhou Z,Zhang Z,Liu D

更新日期：2012-01-01 00:00:00

abstract：:Salazosulfapyridine (SASP) is widely used orally and rectally in the treatment of ulcerative colitis. SASP is mainly metabolized by hydrolysis and the main active metabolite, 5-aminosalicylic acid (5-ASA), has an antiinflammatory effect. In the present study, we prepared suppositories containing 6.5 mmol of SASP and a...

journal_title：Biological & pharmaceutical bulletin

authors： Tokui K,Asai Y,Arakawa T,Matsumoto T,Nabeshima T

更新日期：2002-02-01 00:00:00

abstract：:Cationic lipid-mediated transfer of DNA is promising in gene therapy. However, one disadvantage with this approach is the induction of an inflammatory response, which may decrease transgene expression. Recently, we found that plasmid DNA containing N6-methyladenine (N6-MeA), a bacterium-specific modified base, induced...

journal_title：Biological & pharmaceutical bulletin

authors： Ochiai H,Harashima H,Kamiya H

更新日期：2005-10-01 00:00:00

abstract：:Medicinal plants with reported anti-inflammatory activity could have the potential use as anti-allergens and inhibitors of allergic contact dermatitis reactions produced by allergens and chemicals. Some species from the genus Artocarpus were reported to have anti-inflammatory activity. In the Philippines one local sou...

journal_title：Biological & pharmaceutical bulletin

authors： Salonga RB,Hisaka S,Nose M

更新日期：2014-01-01 00:00:00

abstract：:The photodynamic therapy (PDT) depends on the presence of molecular oxygen. Thus, the efficiency of PDT is limited in anoxic regions of tumor tissue and vascular shutdown. It is reported the use of hyperbaric oxygen (HBO) may enhance the efficiency of PDT. However, there are rarely studies about utilizing HBO plus PDT...

journal_title：Biological & pharmaceutical bulletin

authors： Mei LH,Yang G,Fang F

更新日期：2019-03-01 00:00:00

abstract：:This study aimed to investigate the mechanism underlying the protective effects of manganese complexes of curcumin (Cp-Mn) and diacetylcurcumin (DiAc-Cp-Mn) on kainic acid (KA)-induced excitotoxicity in the rat hippocampus. Systemic injection of KA (10 mg/kg, i.p.) caused seizures and increased the expression of neuro...

journal_title：Biological & pharmaceutical bulletin

authors： Sumanont Y,Murakami Y,Tohda M,Vajragupta O,Watanabe H,Matsumoto K

更新日期：2007-09-01 00:00:00

abstract：:There are many important considerations in the interactions among the herbal constituents in a prescription of traditional Chinese medicine (TCM). Ephedra Herb [Chinese characters: see text] (Eph) is described a warm and acrid agent in TCM. The combination of Eph and Gypsum [Chinese characters: see text] (Eph-Gyp) sho...

journal_title：Biological & pharmaceutical bulletin

authors： Yuan D,Sunouchi H,Sakurai T,Saito K,Kano Y

更新日期：2002-07-01 00:00:00

abstract：:To elucidate molecular mechanisms of adipocyte differentiation, we previously isolated TC10-like/TC10betaLong (TCL/TC10betaL), regulators of G protein signaling 2 (RGS2), factor for adipocyte differentiation (fad) 104 and fad158, which were transiently expressed in the early phase of adipogenesis. These four genes see...

journal_title：Biological & pharmaceutical bulletin

authors： Kawaji A,Nishizuka M,Osada S,Imagawa M

更新日期：2010-01-01 00:00:00

abstract：:Apocynum venetum LINN. is an important Chinese crude drug, and its sibling species A. pictum SCHRENK is a confusable herb which is similar to it. The purpose of this study is to develop DNA molecular markers to distinguish A. venetum from A. pictum through the combinative technologies of bulked segregate analysis (BSA...

journal_title：Biological & pharmaceutical bulletin

authors： Lu C,Zhang W,Peng X,Gu G,Chen M,Tang Z

更新日期：2010-01-01 00:00:00

abstract：:Preparation of the fatty alcohol propylene glycol (FAPG) ointment base plays an important role in controlling the physicochemical properties of ointments. These essay investigates the effects of preparative conditions such as cooling rate and stirring rate on the percutaneous absorption of norfloxacin from FAPG ointme...

journal_title：Biological & pharmaceutical bulletin

authors： Lin HH,Hsu LR,Wu PC,Tsai YH

更新日期：1995-11-01 00:00:00

abstract：:Ge-132 is a synthetic organic germanium that is used as a dietary supplement. The antioxidant activity of Ge-132 on cultured mammalian cells was investigated in this study. First, Ge-132 cytotoxicity on mammalian cultured cells was determined by measuring lactate dehydrogenase (LDH) levels. Ge-132 had no cytotoxic eff...

journal_title：Biological & pharmaceutical bulletin

authors： Wada T,Hanyu T,Nozaki K,Kataoka K,Kawatani T,Asahi T,Sawamura N

更新日期：2018-05-01 00:00:00

abstract：:In brain ischemia, cell destructive necrosis occurs in the core, which in turn links to cell death expansion in the vicinity. Apoptosis, on the other hand, occurs in the surroundings of the core, called the penumbra, several days later. As cells showing apoptosis disappear by microglial phagocytosis in the brain, cell...

journal_title：Biological & pharmaceutical bulletin

authors： Ueda H,Fujita R

更新日期：2004-07-01 00:00:00

abstract：:The effects of hop extracts (Humulus lupulus L.) on histamine release from rat peritoneal mast cells and human basophilic KU812 cells were studied. Hop water extract (HWE) and XAD-4 50% methanol fraction of HWE (MFH) inhibited histamine release from rat mast cells induced by compound 48/80 at concentrations of 100 and...

journal_title：Biological & pharmaceutical bulletin

authors： Takubo M,Inoue T,Jiang S,Tsumuro T,Ueda Y,Yatsuzuka R,Segawa S,Watari J,Kamei C

更新日期：2006-04-01 00:00:00

abstract：:Caesalpinia ferrea MART. (Leguminosae) called as Juca is one of the medicinal plants in Brazil used for diabetes. From the fruits of this plant, ellagic acid (EA) and 2-(2,3,6-trihydroxy-4-carboxyphenyl)ellagic acid (TEA) have been recently isolated as aldose reductase (AR) inhibitors. In this study, we examined to pr...

journal_title：Biological & pharmaceutical bulletin

authors： Ueda H,Kawanishi K,Moriyasu M

更新日期：2004-10-01 00:00:00