当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Depletion of uracil-DNA glycosylase activity is associated with decreased cell proliferation.

Depletion of uracil-DNA glycosylase activity is associated with decreased cell proliferation.

Abstract：

:Uracil-DNA glycosylase (UNG) is the primary enzyme responsible for removing uracil residues from DNA. Increasing evidence suggests that UNG may be a potential target for the development of novel antiviral and/or anticancer agents. To determine whether the uracil-DNA glycosylase inhibitor protein (UGI) could be used to specifically target UNGs intracellularly, we developed a construct that expresses UGI as a fusion protein with the TAT-protein transduction domain and described a novel method for the purification of recombinant TAT-UGI. Treatment of several cell types with TAT-UGI resulted in a dose- and time-dependent decrease in UNG activity. A somewhat surprising effect of TAT-UGI treatment was the decrease in cell proliferation, but not in cell viability. The results of this study support the premise that UNG can be used as a potential therapeutic target and also demonstrate that protein transduction can be used to modulate UNG activity.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Studebaker AW,Ariza ME,Williams MV

doi

10.1016/j.bbrc.2005.06.118

subject

Has Abstract

pub_date

2005-08-26 00:00:00

pages

509-15

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)01375-6

journal_volume

334

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Transport of newly synthesized arylsulfatase A to the lysosome via transferrin receptor-positive compartments.

    abstract：:We have studied the convergence of the biosynthetic lysosomal route marked by the newly synthesized lysosomal enzyme arylsulfatase A (ASA) with the endosomal/prelysosomal compartment in ASA overexpressing baby hamster kidney (BHK) cells. A monoclonal antibody against ASA conjugated to transferrin (Tf-alpha ASA) was us...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2432

    authors： Diesner F,Sommerlade HJ,Braulke T

    更新日期：1993-11-30 00:00:00

  • Chlorpromazine inhibits human natural killer cell activity and antibody-dependent cell-mediated cytotoxicity.

    abstract：:The phenothiazine antipsychotic drug chlorpromazine, a potent calmodulin antagonist, inhibited human natural killer cell-mediated cytotoxicity in a dose-dependent manner. This inhibition of natural killer cell activity was not due to interference with conjugate formation, nor was it due to toxicity to the natural kill...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80536-9

    authors： Steele TA,Brahmi Z

    更新日期：1988-09-15 00:00:00

  • In vivo gene correction with targeted sequence substitution through microhomology-mediated end joining.

    abstract：:Genome editing technology using programmable nucleases has rapidly evolved in recent years. The primary mechanism to achieve precise integration of a transgene is mainly based on homology-directed repair (HDR). However, an HDR-based genome-editing approach is less efficient than non-homologous end-joining (NHEJ). Rece...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.05.130

    authors： Shin JH,Jung S,Ramakrishna S,Kim HH,Lee J

    更新日期：2018-07-07 00:00:00

  • Salivary phospholipid secretion in response to beta-adrenergic stimulation is mediated by Src kinase-dependent epidermal growth factor receptor transactivation.

    abstract：:Communication between receptor tyrosine kinase and G protein-coupled receptor (GPCR)-mediated signaling is recognized as a common integrator linking diverse aspects of intracellular signaling systems. Here, we report that G protein-coupled beta-adrenergic receptor activation leading to stimulation of salivary phosphol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.04.016

    authors： Slomiany BL,Slomiany A

    更新日期：2004-05-21 00:00:00

  • Mast cell chemotactic activity of RANTES.

    abstract：:RANTES is a cytokine produced by activated T-lymphocytes that has been shown to exert chemotactic activity for memory-type CD4 T-lymphocytes and eosinophils. In this study, RANTES caused directional migration of human mast cells. When compared to other potential chemoattractants of the same cells, RANTES was found to ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1505

    authors： Mattoli S,Ackerman V,Vittori E,Marini M

    更新日期：1995-04-06 00:00:00

  • Prominin-2 expression increases protrusions, decreases caveolae and inhibits Cdc42 dependent fluid phase endocytosis.

    abstract：BACKGROUND:Membrane protrusions play important roles in biological processes such as cell adhesion, wound healing, migration, and sensing of the external environment. Cell protrusions are a subtype of membrane microdomains composed of cholesterol and sphingolipids, and can be disrupted by cholesterol depletion. Promini...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.03.097

    authors： Singh RD,Schroeder AS,Scheffer L,Holicky EL,Wheatley CL,Marks DL,Pagano RE

    更新日期：2013-05-10 00:00:00

  • Effects of dietary eicosapentaenoate (20:5 n-3) on cardiac beta-adrenergic receptor activity in the marmoset monkey.

    abstract：:Eicosapentaenoic acid (EPA; 20:5 n-3; ethyl ester) in combination with atherogenic or non-atherogenic high fat diets was fed to young adult male marmoset monkeys for a period of 30 weeks. EPA markedly reduced the raised plasma cholesterol level evident when feeding an atherogenic diet but did not influence the cardiac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92365-6

    authors： Patten GS,Rinaldi JA,McMurchie EJ

    更新日期：1989-07-31 00:00:00

  • Corticotropin-releasing hormone activates protein kinase C in an isoenzyme-specific manner.

    abstract：:Protein kinase C (PKC) has recently emerged as mediator of corticotropin-releasing hormone (CRH) effects. Aim of the present study was to study the effects of CRH on each PKC isoenzyme. As a model we have used the PC12 rat pheochromocytoma cell line, expressing the CRH type 1 receptor (CRHR1). Our data were as follows...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.12.078

    authors： Dermitzaki E,Tsatsanis C,Charalampopoulos I,Androulidaki A,Alexaki VI,Castanas E,Gravanis A,Margioris AN

    更新日期：2005-02-18 00:00:00

  • Deletion-mutant epidermal growth factor receptor in human gliomas: effects of type II mutation on receptor function.

    abstract：:Malignant human glioma D-298 MG amplifies a rearranged epidermal growth factor receptor (EGFR) gene (c-erbB proto-oncogene), resulting in an in-frame deletion of 83 amino acids in domain IV of the extracellular domain of the EGFR. EGF and transforming growth factor-a (TGF-a) bound to the mutant EGFR with high affinity...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91051-d

    authors： Humphrey PA,Gangarosa LM,Wong AJ,Archer GE,Lund-Johansen M,Bjerkvig R,Laerum OD,Friedman HS,Bigner DD

    更新日期：1991-08-15 00:00:00

  • A histone 1-like antigen is a component of the nuclear envelope.

    abstract：:Rabbit antibodies have been raised against rat liver nuclear envelopes. An enzyme-linked immunosorbent assay (ELISA) demonstrated high titer antiserum specific for the nuclear envelope preparation. Immunocytochemical studies showed that the antiserum stained the nuclear envelopes, but not intra-nuclear components of H...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80895-7

    authors： Venkatraman JT,Gohill J,Fritzler MJ,Lefebvre YA

    更新日期：1988-10-31 00:00:00

  • Macrophage-mediated N-nitrosation of thioproline and proline.

    abstract：:Thioproline (Thiazolidine-4-carboxylic acid) and proline were nitrosated by stimulated mouse macrophages in vitro. A macrophage cell line (J774.1, 1.0 x 10(6)/well, 1 ml) was incubated with Escherichia coli lipopolysaccharide, interferon-gamma and thioproline (5 mM) or proline (5 mM). After 72 hr incubation at 37 degr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)90001-6

    authors： Miwa M,Tsuda M,Kurashima Y,Hara H,Tanaka Y,Shinohara K

    更新日期：1989-03-15 00:00:00

  • Aggregation state of the gonadotropin receptor.

    abstract：:The aggregation state of the gonadotropin receptor has been examined by coupling fluorescence energy transfer donor and acceptor fluorophores to hCG and LH. Energy transfer is observed at low (4 degrees C) but not at high (37 degrees C) temperature. Energy transfer could also be detected with receptor solubilized in t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(85)80216-3

    authors： Harmatz D,Ji TH,Middaugh CR

    更新日期：1985-03-15 00:00:00

  • Baicalein attenuates acute liver injury by blocking NLRP3 inflammasome.

    abstract：:Infection and/or drug-mediated acute liver injury, the leading cause of lethal liver failure, is a critical health problem worldwide and lacks effective treatment. Here, we used Lipopolysaccharides (LPS)/D-galactosamine (D-gal)-treated primary hepatocytes to screen a natural library that contains 1130 chemicals. Baica...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.11.109

    authors： Xiao T,Cui Y,Ji H,Yan L,Pei D,Qu S

    更新日期：2021-01-01 00:00:00

  • Zinc-binding protein-89 (ZBP-89) cooperates with NF-κB to regulate expression of matrix metalloproteinases (MMPs) in response to inflammatory cytokines.

    abstract：:Matrix metalloproteinases (MMPs) have both protective and pathological roles in inflammation, and transcriptional mechanisms are important in regulating physiological levels to maintain health. Zinc-binding protein-89 (ZBP-89) is a transcription factor with roles in regulating vital cellular processes, acting through ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.02.045

    authors： Borghaei RC,Gorski G,Seutter S,Chun J,Khaselov N,Scianni S

    更新日期：2016-03-18 00:00:00

  • The modulation of growth by HMBA in PKC overproducing HT29 colon cancer cells.

    abstract：:To examine whether protein kinase C (PKC) plays a role in mediating growth inhibitory effects of hexamethylene bisacetamide (HMBA) we compared a control H29 colon cancer cell line to a derivative, HT29-PKC7, that overexpresses high levels of PKC beta 1. We found that although HMBA markedly inhibited the growth of the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91262-b

    authors： Choi PM,Weinstein IB

    更新日期：1991-12-16 00:00:00

  • The electrophysiological expression of Ca2+ channels and of apamin sensitive Ca2+ activated K+ channels is abolished in skeletal muscle cells from mice with muscular dysgenesis.

    abstract：:Action potentials of myotubes in culture prepared from 18-19 day -old mouse embryos have a contractile activity and action potentials that are followed by a long lasting after hyperpolarization (ahp) which is blocked by apamin. Myotubes prepared from embryos of mice with muscular dysgenesis (mdg/mdg) did not contract ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90422-5

    authors： Romey G,Rieger F,Renaud JF,Pinçon-Raymond M,Lazdunski M

    更新日期：1986-05-14 00:00:00

  • HSP-1/2, a major protein of equine seminal plasma, exhibits chaperone-like activity.

    abstract：:The major bovine seminal plasma protein, PDC-109 exhibits chaperone-like activity (CLA) against a variety of target proteins. The present studies show that the homologous protein from equine seminal plasma, HSP-1/2 also exhibits CLA and inhibits the thermal aggregation of target proteins such as lactate dehydrogenase,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.08.120

    authors： Sankhala RS,Kumar CS,Singh BP,Arangasamy A,Swamy MJ

    更新日期：2012-10-12 00:00:00

  • Deficient repair of 8-hydroxyguanine in the BxPC-3 pancreatic cancer cell line.

    abstract：:Elevated levels of oxidatively induced DNA lesions have been reported in malignant pancreatic tissues relative to normal pancreatic tissues. However, the ability of the pancreatic cancer cells to remove these lesions has not previously been addressed. This study analyzed the effectiveness of the pancreatic cancer cell...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.08.134

    authors： Nyaga SG,Lohani A,Evans MK

    更新日期：2008-11-14 00:00:00

  • Aminoethylcysteine ketimine decarboxylated dimer inhibits mitochondrial respiration by impairing electron transport at complex I level.

    abstract：:The product of the spontaneous dimerization and decarboxylation of aminoethylcysteine ketimine (simply named the dimer in this note) has been investigated for a possible biochemical activity. It has been found that the dimer inhibits the ADP-dependent oxidation of NAD(+)-linked substrates in rat liver mitochondria and...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1293

    authors： Pecci L,Montefoschi G,Fontana M,Cavallini D

    更新日期：1994-03-15 00:00:00

  • Transmission of human TT virus of genotype 1a to chimpanzees with fecal supernatant or serum from patients with acute TTV infection.

    abstract：:Fecal supernatant or serum containing TT virus (TTV) of genotype 1a (10(5) copies/ml) from patients with acute TTV infection was inoculated intravenously into two naive chimpanzees. Serum samples were obtained weekly and tested for TTV DNA by genotype 1-specific polymerase chain reaction. TTV DNA was detected in chimp...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3825

    authors： Tawara A,Akahane Y,Takahashi M,Nishizawa T,Ishikawa T,Okamoto H

    更新日期：2000-11-19 00:00:00

  • The carboxy-terminal end of the peptide deformylase from Mycobacterium tuberculosis is indispensable for its enzymatic activity.

    abstract：:The peptide deformylase in bacteria is involved in removal of N-formyl group from newly synthesized proteins. The gene encoding this enzyme from Mycobacterium tuberculosis was cloned and expressed in Escherichia coli. The enzyme activity of the recombinant protein (mPDF) was insensitive to modulation by common monoval...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.04.142

    authors： Saxena R,Chakraborti PK

    更新日期：2005-07-01 00:00:00

  • Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos.

    abstract：:The monoclonal antibody 6E2 raised against the embryonal carcinoma cell line NCR-G3 had been shown to also react with human germ cells. Thin-layer chromatography (TLC) immunostaining revealed that 6E2 specifically reacts with sialosylglobopenta osylceramide (sialylGb5), which carries an epitope of stage-specific embry...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.10.093

    authors： Sato B,Katagiri YU,Miyado K,Akutsu H,Miyagawa Y,Horiuchi Y,Nakajima H,Okita H,Umezawa A,Hata J,Fujimoto J,Toshimori K,Kiyokawa N

    更新日期：2007-12-28 00:00:00

  • Characterization of the human PCBD gene encoding the bifunctional protein pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor for the transcription factor HNF-1 alpha.

    abstract：:The human pterin-4 alpha-carbinolamine dehydratase (PCD)/dimerization cofactor for the transcription factor HNF-1 alpha is a bifunctional protein proposed to be involved in entirely different biochemical functions. We previously established the complete amino acid sequence for the human liver PCD and subsequently isol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1751

    authors： Thöny B,Neuheiser F,Blau N,Heizmann CW

    更新日期：1995-05-25 00:00:00

  • MEKA/phosducin attenuates hydrophobicity of transducin beta gamma subunits without binding to farnesyl moiety.

    abstract：:Hydrophobic modifications of transducin (T) gamma, such as farnesyl-and carboxyl-methylation, are essential for the association of T beta gamma with the photoreceptor disc membrane, and MEKA/phosducin is known to inhibit the association. In this study, we examined the effect of MEKA on the hydrophobicity of T beta gam...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0939

    authors： Tanaka H,Kuo CH,Matsuda T,Fukada Y,Hayashi F,Ding Y,Irie Y,Miki N

    更新日期：1996-06-25 00:00:00

  • Involvement of Gi/o in the PAR-4-induced NO production in endothelial cells.

    abstract：:We investigated the involvement of G(i/o) protein in NO production following the activation of proteinase-activated receptor-4 (PAR-4) in cultured bovine aortic endothelial cells. AYPGKF-NH(2) (PAR-4 activating peptide), thrombin, and ionomycin induced a concentration-dependent NO production, with the maximal producti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.01.165

    authors： Momota F,Hirano K,Hirano M,Nishimura J,Kanaide H

    更新日期：2006-04-07 00:00:00

  • Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis.

    abstract：:Epigenetic modifications such as DNA methylation and histone H3 lysine 27 methylation (H3K27me) are repressive marks that silence gene expression. The M phase phosphoprotein (MPP8) associates with proteins involved in both DNA methylation and histone modifications, and therefore, is a potential candidate to mediate cr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.01.122

    authors： Murata K,Sato S,Haruta M,Goshima T,Chiba Y,Takahashi S,Sharif J,Koseki H,Nakanishi M,Shimada M

    更新日期：2015-03-13 00:00:00

  • Brain cancer stem-like cell genesis from p53-deficient mouse astrocytes by oncogenic Ras.

    abstract：:Here, we show that H-ras(V12) causes the p53-knockout mouse astrocytes (p53-/- astrocytes) to be transformed into brain cancer stem-like cells. H-ras(V12) triggers the p53-/- astrocytes to express a Nestin and a Cd133, which are expressed in normal and cancer neural stem cells. H-ras(V12) also induces the formation of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.11.005

    authors： Lee JS,Gil JE,Kim JH,Kim TK,Jin X,Oh SY,Sohn YW,Jeon HM,Park HJ,Park JW,Shin YJ,Chung YG,Lee JB,You S,Kim H

    更新日期：2008-01-18 00:00:00

  • USP39, a direct target of microRNA-133a, promotes progression of pancreatic cancer via the AKT pathway.

    abstract：:Ubiquitin specific protease 39 (USP39) is one of the deubiquitinating enzymes without ubiquitin protease activity, which has been implicated in the progression of several cancers. However, the role of USP39 in pancreatic cancer (PC) is largely unknown. In present study, we found that USP39 expression was elevated in P...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.03.025

    authors： Cai J,Liu T,Huang P,Yan W,Guo C,Xiong L,Liu A

    更新日期：2017-04-22 00:00:00

  • Quercetin restrains TGF-β1-induced epithelial-mesenchymal transition by inhibiting Twist1 and regulating E-cadherin expression.

    abstract：:Emerging evidence has indicated that transforming growth factor-beta 1 (TGF-β1) induces the epithelial-mesenchymal transition (EMT) in cancer cells, thus promoting their motility and invasiveness. Quercetin, a member of the polyphenolic flavonoid family, has been reported to display anticancer activity against a broad...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.02.044

    authors： Feng J,Song D,Jiang S,Yang X,Ding T,Zhang H,Luo J,Liao J,Yin Q

    更新日期：2018-03-25 00:00:00

  • Cell type-specific modulation of lipid mediator's formation in murine adipose tissue by omega-3 fatty acids.

    abstract：:Mutual interactions between adipocytes and immune cells in white adipose tissue (WAT) are involved in modulation of lipid metabolism in the tissue and also in response to omega-3 polyunsaturated fatty acids (PUFA), which counteract adverse effects of obesity. This complex interplay depends in part on in situ formed an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.12.055

    authors： Kuda O,Rombaldova M,Janovska P,Flachs P,Kopecky J

    更新日期：2016-01-15 00:00:00