Rapid identification of emerging pathogens: coronavirus.

Abstract:

:We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to identify organisms in a sample. We demonstrate this principle by using 14 isolates of 9 diverse Coronavirus spp., including the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). We show that this method could identify and distinguish between SARS and other known CoV, including the human CoV 229E and OC43, individually and in a mixture of all 3 human viruses. The sensitivity of detection, measured by using titered SARS-CoV spiked into human serum, was approximate, equals1 PFU/mL. This approach, applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens, is capable of automated analysis of >900 PCR reactions per day.

journal_name

Emerg Infect Dis

authors

Sampath R,Hofstadler SA,Blyn LB,Eshoo MW,Hall TA,Massire C,Levene HM,Hannis JC,Harrell PM,Neuman B,Buchmeier MJ,Jiang Y,Ranken R,Drader JJ,Samant V,Griffey RH,McNeil JA,Crooke ST,Ecker DJ

doi

10.3201/eid1103.040629

subject

Has Abstract

pub_date

2005-03-01 00:00:00

pages

373-9

issue

3

eissn

1080-6040

issn

1080-6059

journal_volume

11

pub_type

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