Abstract:
:We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to identify organisms in a sample. We demonstrate this principle by using 14 isolates of 9 diverse Coronavirus spp., including the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). We show that this method could identify and distinguish between SARS and other known CoV, including the human CoV 229E and OC43, individually and in a mixture of all 3 human viruses. The sensitivity of detection, measured by using titered SARS-CoV spiked into human serum, was approximate, equals1 PFU/mL. This approach, applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens, is capable of automated analysis of >900 PCR reactions per day.
journal_name
Emerg Infect Disjournal_title
Emerging infectious diseasesauthors
Sampath R,Hofstadler SA,Blyn LB,Eshoo MW,Hall TA,Massire C,Levene HM,Hannis JC,Harrell PM,Neuman B,Buchmeier MJ,Jiang Y,Ranken R,Drader JJ,Samant V,Griffey RH,McNeil JA,Crooke ST,Ecker DJdoi
10.3201/eid1103.040629subject
Has Abstractpub_date
2005-03-01 00:00:00pages
373-9issue
3eissn
1080-6040issn
1080-6059journal_volume
11pub_type
杂志文章abstract::In China, domestic ducks and wild birds often share the same water, in which influenza viruses replicate preferentially. Isolation of 2 novel reassortant highly pathogenic avian influenza (H5N5) viruses from apparently healthy domestic ducks highlights the role of these ducks as reassortment vessels. Such new subtypes...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid/1706.101406
更新日期:2011-06-01 00:00:00
abstract::In August 2003, a communitywide outbreak of cryptosporidiosis occurred in Kansas. We conducted a case-control study to assess risk factors associated with Cryptosporidium infection by using the telephone survey infrastructure of the Behavioral Risk Factor Surveillance System. Using existing state-based infrastructure ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1105.040871
更新日期:2005-05-01 00:00:00
abstract::To determine the proportion of Escherichia coli O157 cases in Scotland attributable to secondary spread, we analyzed data obtained through entire-population enhanced surveillance. We identified 11% of cases as secondary. Secondary cases in single households were younger than secondary cases in outbreaks affecting >1 h...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1703.100167
更新日期:2011-03-01 00:00:00
abstract::Using data from the 2000-2004 US Healthcare Cost and Utilization Project National Inpatient Sample, we found that total hospital admissions for skin and soft tissue infections increased by 29% during 2000-2004; admissions for pneumonia were largely unchanged. These results are consistent with recent reported increases...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1509.081228
更新日期:2009-09-01 00:00:00
abstract::Despite the considerable number of women in the United States who identify as lesbian, few data exist that address lesbians' health needs. The Institute of Medicine emphasized that data on sexually transmitted infections, Pap smear screening, and cervical dysplasia among lesbians were needed to guide clinical practice...
journal_title:Emerging infectious diseases
pub_type: 杂志文章,评审
doi:10.3201/eid1011.040467
更新日期:2004-11-01 00:00:00
abstract::A multidrug-resistant Salmonella enterica serotype Anatum strain reported in Taiwan was isolated in the United States from patients and from seafood imported from Asia. Isolates harbored 11 resistance determinants, including quinolone and inducible cephalosporin resistance genes. Most patients had traveled to Asia. Th...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2605.190992
更新日期:2020-05-01 00:00:00
abstract::Human infections with vaccinia virus (VACV), mostly from laboratory accidents or contact with infected animals, have occurred since smallpox was eradicated in 1980. No recent cases have been reported in China. We report on an outbreak of VACV from occupational exposure to rabbit skins inoculated with VACV. ...
journal_title:Emerging infectious diseases
pub_type: 历史文章,杂志文章
doi:10.3201/eid2506.171306
更新日期:2019-06-01 00:00:00
abstract::Serologic studies for swine influenza viruses (SIVs) in humans with occupational exposure to swine have been reported from the Americas but not from Europe. We compared levels of neutralizing antibodies against 3 influenza viruses--pandemic (H1N1) 2009, an avian-like enzootic subtype H1N1 SIV, and a 2007-08 seasonal s...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1703.100851
更新日期:2011-03-01 00:00:00
abstract::Dengue virus type 1 (DENV-1), which was responsible for the dengue fever outbreak in Yap State, Micronesia, in 2004, was isolated from serum samples of 4 dengue patients in Japan. Genome sequencing demonstrated that this virus belonged to genotype IV and had a 29-nucleotide deletion in the 3 noncoding region. ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1202.050733
更新日期:2006-02-01 00:00:00
abstract::After a human rabies outbreak in southeastern Peru, we collected bats to estimate the prevalence of rabies in various species. Among 165 bats from 6 genera and 10 species, 10.3% were antibody positive; antibody prevalence was similar in vampire and nonvampire bats. Thus, nonvampire bats may also be a source for human ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1508.081522
更新日期:2009-08-01 00:00:00
abstract::From 1992 to 1999, we analyzed >2.5 million cases of influenzalike illness (ILI). Nationwide influenza epidemics generally lasted 3-4 months in winter. Kriging analysis, which illustrates geographic movement, showed that the starting areas of peak ILI activity were mostly found in western Japan. Two spreading patterns...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1010.040147
更新日期:2004-10-01 00:00:00
abstract::Chagas disease, caused by Trypanosoma cruzi, is a major neglected tropical disease affecting the Americas. The epidemiology of this disease in the United States is incomplete. We report evidence of likely autochthonous vectorborne transmission of T. cruzi and health outcomes in T. cruzi-seropositive blood donors in so...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2303.161157
更新日期:2017-03-01 00:00:00
abstract::A recent Hendra virus outbreak at a veterinary clinic in Brisbane, Queensland, Australia, involved 5 equine and 2 human infections. In contrast to previous outbreaks, infected horses had predominantly encephalitic, rather than respiratory, signs. After an incubation period of 9-16 days, influenza-like illnesses develo...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1602.090552
更新日期:2010-02-01 00:00:00
abstract::To characterize parapoxviruses causing severe disease in wild ruminants in Stelvio Park, Italy, we sequenced and compared the DNA of several isolates. Results demonstrated that the red deer isolates are closely related to the parapox of red deer in New Zealand virus. ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1704.101454
更新日期:2011-04-01 00:00:00
abstract::Influenza A (H5N1) viruses are strong candidates for causing the next influenza pandemic if they acquire the ability for efficient human-to-human transmission. A major public health goal is to make efficacious vaccines against these viruses by using novel approaches, including cell-culture system, reverse genetics, an...
journal_title:Emerging infectious diseases
pub_type: 社论
doi:10.3201/eid1310.061262
更新日期:2007-10-01 00:00:00
abstract::Faced with an epidemic of an infectious disease, persons may take precautionary actions to try to reduce their risk. Such actions include avoiding situations that persons perceive to be risky, which can have negative health and economic effects. Therefore, we conducted a population-based survey of persons' precautiona...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1309.070372
更新日期:2007-09-01 00:00:00
abstract::We report 13 cases of Naegleria fowleri primary amebic meningoencephalitis in persons in Karachi, Pakistan, who had no history of aquatic activities. Infection likely occurred through ablution with tap water. An increase in primary amebic meningoencephalitis cases may be attributed to rising temperatures, reduced leve...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1702.100442
更新日期:2011-02-01 00:00:00
abstract::Available animal and cell line models have suggested that specific therapeutics might be effective in treating Middle East respiratory syndrome (MERS). We conducted a systematic review of evidence for treatment with pharmacologic and supportive therapies. We developed a protocol and searched 5 databases for studies de...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2606.200037
更新日期:2020-06-01 00:00:00
abstract::We determined the seroprevalence of hepatitis E virus (HEV) in persons in 2 rural communities in southeastern Bolivia and the presence of HEV in human and swine fecal samples. HEV seroprevalence was 6.3%, and HEV genotype 3 strains with high sequence homology were detected. ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1708.100769
更新日期:2011-08-01 00:00:00
abstract::Vellore, a region in southern India, has a high incidence of severe human infections with Beta-hemolytic group C and G streptococci (GCGS). To determine the causative species in these infections, we conducted 16S rRNA gene sequencing: Streptococcus dysgalactiae subsp. equisimilis (81%) and S. anginosus (19%) were the ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1604.090448
更新日期:2010-04-01 00:00:00
abstract::Pandemic (H1N1) 2009 affected Chile during the winter of 2009. The hospitalization rate was 0.56% overall and 3.47% for persons >60 years of age at risk for severe disease and death independent of concurrent conditions. Age >60 years was the major risk factor for death from pandemic (H1N1) 2009. ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1707.101398
更新日期:2011-07-01 00:00:00
abstract::A sentinel group of primary-care physicians in Wales actively reported cases of acute bloody diarrhea from February 1997 through December 1998. The estimated annual rate was 18 cases per 100,000 population. Most (80%) cases were due to Campylobacter or Salmonella; however, 18% were undiagnosed. ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid0604.000418
更新日期:2000-07-01 00:00:00
abstract::We conducted a retrospective study of Salmonella Newport infections among Wisconsin residents during 2003-2005. Multidrug resistance prevalence was substantially greater in Wisconsin than elsewhere in the United States. Persons with multidrug-resistant infections were more likely than persons with susceptible infectio...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1311.061138
更新日期:2007-11-01 00:00:00
abstract::To detect orthopoxvirus in the Brazilian Amazon, we conducted a serosurvey of 344 wild animals. Neutralizing antibodies against orthopoxvirus were detected by plaque-reduction neutralizing tests in 84 serum samples. Amplicons from 6 monkey samples were sequenced. These amplicons identified vaccinia virus genetically s...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1606.091187
更新日期:2010-06-01 00:00:00
abstract::Since Ebola virus disease was identified in West Africa on March 23, 2014, the Centers for Disease Control and Prevention (CDC) has undertaken the most intensive response in the agency's history; >3,000 staff have been involved, including >1,200 deployed to West Africa for >50,000 person workdays. Efforts have include...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2111.150949
更新日期:2015-11-01 00:00:00
abstract::Genetic characterization of highly pathogenic avian influenza viruses (H5N1) isolated in July 2008 in Nigeria indicates that a distinct genotype, never before detected in Africa, reached the continent. Phylogenetic analysis showed that the viruses are genetically closely related to European and Middle Eastern influenz...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1503.081161
更新日期:2009-03-01 00:00:00
abstract::Transmissible mink encepholapathy (TME) is a foodborne transmissible spongiform encephalopathy (TSE) of ranch-raised mink; infection with a ruminant TSE has been proposed as the cause, but the precise origin of TME is unknown. To compare the phenotypes of each TSE, bovine-passaged TME isolate and 3 distinct natural bo...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1312.070635
更新日期:2007-12-01 00:00:00
abstract::Cross-sectional studies suggest an increasing trend in incidence and relatively low recurrence rates of Clostridium difficile infections in Asia than in Europe and North America. The temporal trend of C. difficile infection in Asia is not completely understood. We conducted a territory-wide population-based observatio...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2310.170797
更新日期:2017-10-01 00:00:00
abstract::Guidelines for Preventing Opportunistic Infections Among Hematopoietic Stem Cell Transplant Recipients contains a section on hospital infection control including evidence-based recommendations regarding ventilation, construction, equipment, plants, play areas and toys, health-care workers, visitors, patient skin and o...
journal_title:Emerging infectious diseases
pub_type: 杂志文章,评审
doi:10.3201/eid0702.010223
更新日期:2001-03-01 00:00:00
abstract::To determine the prevalence of helminthic infections in Pursat Province, Cambodia, we tested fecal specimens from 471 children, 10-14 years of age, in June 2007. The prevalence of infection with echinostome flukes ranged from 7.5% to 22.4% in 4 schools surveyed. Adult worms were identified as Echinostoma revolutum. ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1701.100920
更新日期:2011-01-01 00:00:00