Abstract：
CONTEXT:The pyrimidine nucleoside analog, cytosine arabinoside (Ara-C), is an effective therapeutic agent for acute leukemia. The phosphorylated triphosphate, cytosine arabinoside triphosphate, competes with deoxycytosine triphosphate as a substrate for incorporation into DNA. Once incorporated into DNA, it inhibits DNA polymerase and topoisomerase I and modifies the tertiary structure of DNA. OBJECTIVE:To determine if the substitution of Ara-C for cytosine in double-stranded oligonucleotides that contain 4 specific transcription factor binding sites (TATA, GATA, C/EBP, and AP-2alpha) alters transcription factor binding to their respective DNA binding elements. DESIGN:Transcription factors were obtained from nuclear extracts from human promyelocytic leukemia HL-60 cells. [32P]-end-labeled double-stranded oligonucleotides that contained 1 or 2 specific transcription factor binding sites with or without Ara-C substitution for cytosine were used to assess transcription factor binding by electrophoretic mobility shift assay. RESULTS:The substitution of Ara-C for cytosine within and outside the transcription factor binding element (AP-2alpha, C/EBP), outside the binding element only (GATA, TATA), or within the binding element only (AP-2alpha) all result in a reduction in transcription factor binding to their respective DNA binding element. CONCLUSION:The reduction of the binding capacity of transcription factors with their respective DNA binding elements may depend on structural changes within oligonucleotides induced by Ara-C incorporation. This altered binding capacity of transcription factors to their DNA binding elements may represent one mechanism for Ara-C cytotoxicity secondary to inhibition of transcription of new messenger RNAs and, subsequently, translation of new proteins.
journal_name
Arch Pathol Lab Medjournal_title
Archives of pathology & laboratory medicineauthors
Zhang X,Kiechle FLdoi
10.1043/1543-2165(2004)128<1364:CASDTF>2.0.CO;2subject
Has Abstractpub_date
2004-12-01 00:00:00pages
1364-71issue
12eissn
0003-9985issn
1543-2165pii
AS3283journal_volume
128pub_type
杂志文章abstract：CONTEXT:Success in the practice of pathology demands proficiency in management, but management training for pathology residents is generally inadequate, with little agreement on an appropriate curriculum or competency assessment. Most residency training programs do not have faculty members who are interested and have e...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/1543-2165(2004)128<59:MTFPR>2.0.CO;2
更新日期：2004-01-01 00:00:00
abstract：:The costs of producing quality medical care and the proper allocation of resources to assure quality are major contemporary concerns. Minimalists attempt to improve quality and lower costs through the application of the theory, "less is more." Bureaucracies make the same attempt through the denial of payments for "unn...
journal_title：Archives of pathology & laboratory medicine
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doi：
更新日期：1990-11-01 00:00:00
abstract：CONTEXT:-Whole-slide imaging technology offers promise for rapid, Internet-based telepathology consultations between institutions. Before implementation, technical issues, pathologist adaptability, and morphologic pitfalls must be well characterized. OBJECTIVE:-To determine whether interpretation of whole-slide images...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/1543-2165-133.12.1949
更新日期：2009-12-01 00:00:00
abstract：:The fluorescent dye thioflavin T stains intensely the deposits in dense deposit disease and can be used for identification purposes. Comparison of thioflavin T staining with electron microscopy was carried out in 25 patients and showed excellent congruity. Thioflavin T is much easier to use, but is less specific than ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1979-02-01 00:00:00
abstract：CONTEXT:Eighty-two million surgical pathology and cytology reports were issued in the United States during 2007; a subset of these reports will be misunderstood by readers. Recent attention has focused on standardizing the content of pathology reports, particularly for common malignancies, to facilitate transmission of...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.1043/1543-2165(2008)132[84:FPRAFD]2.0.CO;2
更新日期：2008-01-01 00:00:00
abstract：:Since it is believed that "a picture is worth a thousand words," incorporation of computer-generated line art was used as a adjunct to gross description in surgical pathology reporting in selected cases. The lack of an integrated software program was overcome by using commercially available graphic and word processing...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:The significance of an elevated free thyroxine index (FTI) as an indicator of hyperthyroidism was studied while screening 651 elderly nursing home residents. Eleven subjects had FTI elevations. Most of these patients were chronically ill and/or malnourished. Clinical assessment, repeated FTI determinations, and subseq...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Three patients with congenital partial absence of the right ventricular myocardium were studied. These cases are unique in that all three patients died suddenly and none had clinical evidence of cardiovascular disease. Two of the three patients were active in sports, and both died suddenly while playing basketball. At...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1982-04-01 00:00:00
abstract：:Twenty chordomas from 20 patients, including 17 nonchondroid and three chondroid types, were studied with a variety of antibodies directed against cytokeratin (AE-1/3), epithelial membrane antigen, carcinoembryonic antigen, S100 protein, vimentin, alpha 1-antichymotrypsin, and lysozyme. All 17 nonchondroid chordomas s...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：CONTEXT:Cystatin C is becoming an increasingly popular biomarker for estimating glomerular filtration rate, and accurate measurements of cystatin C concentrations are necessary for accurate estimates of glomerular filtration rate. OBJECTIVE:To assess the accuracy of cystatin C concentration measurements in laboratorie...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.5858/arpa.2014-0427-CP
更新日期：2015-07-01 00:00:00
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journal_title：Archives of pathology & laboratory medicine
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更新日期：2008-08-01 00:00:00
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journal_title：Archives of pathology & laboratory medicine
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journal_title：Archives of pathology & laboratory medicine
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doi：10.1043/1543-2165(2003)127<1509:MFOSRC>2.0.CO;2
更新日期：2003-11-01 00:00:00
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journal_title：Archives of pathology & laboratory medicine
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doi：10.5858/arpa.2012-0733-OA
更新日期：2014-01-01 00:00:00
abstract：:Hepatic tumors unassociated with cirrhosis were encountered in seven patients aged 10 to 19 years. Four patients had received androgens for aplastic anemia. Two patients had transfusional hemosiderosis. One patient had had a renal transplant 2 1/2 years ago. Two patients are alive at 2 3/4 and 2 1/2 years after surgic...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1984-02-01 00:00:00
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journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:We investigated how route of infection with Treponema pallidum affects cellular responses of various lymphoid tissues. Lymphoid cells from spleens and lymph nodes of rabbits infected intradermally with T pallidum showed enhanced in vitro responses to treponemal antigens when cultured in autologous rabbit serum. Intrav...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1982-09-01 00:00:00
abstract：:Rapid tissue diagnosis during acute events associated with renal transplantation is often necessary to permit immediate decisions regarding initiation or modification of therapy. To achieve this, we performed frozen section analyses with cryoprotected alcoholic Bouin's-fixed tissue, which permits evaluation within 2.5...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:Critical values in anatomic pathology are usually information sensitive, whereas most such values in laboratory medicine are time sensitive. However, there is an important time element in anatomic pathology as well. Pathologists should be aware that many medicolegal actions against radiologists are based on failure to...
journal_title：Archives of pathology & laboratory medicine
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doi：10.1043/1543-2165(2006)130[641:CCVIAP]2.0.CO;2
更新日期：2006-05-01 00:00:00
abstract：CONTEXT:Cervical cancer affects 12 000 women in the United States annually. However, despite its prevalence, there remains no good methodology to detect its recurrence. OBJECTIVE:To identify the role of cervicovaginal high-risk human papilloma virus (hr-HPV) testing in predicting cervical cancer recurrence. DESIGN:Th...
journal_title：Archives of pathology & laboratory medicine
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doi：10.5858/arpa.2014-0534-OA
更新日期：2015-11-01 00:00:00
abstract：:We recently encountered an elderly female patient with multiple, rapidly growing vascular lesions on her forearm. Histologic examination and immunoperoxidase studies were performed to identify cells present within the lesions. The growths proved to be intravascular papillary endothelial hyperplasia. Immunohistochemica...
journal_title：Archives of pathology & laboratory medicine
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doi：
更新日期：1994-03-01 00:00:00
abstract：:An unprecedented presentation of multicentric paraganglioma in a 48-year-old man is described. One of the paragangliomas, originally diagnosed as a carcinoid tumor, presented as a lung mass and was removed. Four years later, an intravagal paraganglioma was discovered. The lung and intravagal tumors had identical morph...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Primary malignant deciduoid mesothelioma is a rare subtype of epithelioid mesothelioma that was first described in the peritoneum in young women without a history of asbestos exposure. It was thought to be a distinct clinicopathologic entity with ominous prognosis; recent studies have better characterized this entity....
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2017-0461-RS
更新日期：2019-04-01 00:00:00
abstract：CONTEXT:Clinical laboratory specimens may be rejected as unsuitable for analysis for a variety of reasons and specimen rejection may have significant clinical consequences. OBJECTIVE:To quantify the clinical consequences of specimen rejection and determine the impact of laboratories' policies and practices on these co...
journal_title：Archives of pathology & laboratory medicine
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doi：10.5858/arpa.2013-0331-CP
更新日期：2014-08-01 00:00:00
abstract：CONTEXT:- Malignant mesothelioma (MM) is an uncommon tumor that can be difficult to diagnose. OBJECTIVE:- To provide updated, practical guidelines for the pathologic diagnosis of MM. DATA SOURCES:- Pathologists involved in the International Mesothelioma Interest Group and others with an interest and expertise in the ...
journal_title：Archives of pathology & laboratory medicine
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doi：10.5858/arpa.2017-0124-RA
更新日期：2018-01-01 00:00:00
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pub_type： 共识发展会议,杂志文章
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更新日期：2013-02-01 00:00:00
abstract：:A 38-year-old woman suffered rapid onset of renal failure between 11 and 15 months after undergoing a jejunoileal bypass for morbid obesity. Microscopic examination of renal biopsy specimens revealed oxalosis and severe tubulointerstitial nephritis. Immunofluorescence microscopy disclosed linear staining of tubular ba...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1981-07-01 00:00:00
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journal_title：Archives of pathology & laboratory medicine
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doi：10.1043/0003-9985(2003)127<169:AALCFH>2.0.CO;2
更新日期：2003-02-01 00:00:00
abstract：:The Health Care Financing Administration of the US Department of Health and Human Services is the federal agency that has the responsibility for the administration of the Medicare and Clinical Laboratories Improvement Act of 1967 programs, including the promulgation of standards for clinical laboratories. The Health C...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:A 35-year-old man with hematuria had a polypoid bladder lesion, which on microscopic examination was a fibroepithelial polyp containing numerous stromal cells with atypical, hyperchromatic nuclei. The polyp resembled those encountered in the lower female genital tract and other sites and must be distinguished from sar...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
