Asiaticoside induction for cell-cycle progression, proliferation and collagen synthesis in human dermal fibroblasts.

Abstract:

:Asiaticoside, isolated from Centella asiatica, promotes fibroblast proliferation and extracullar matrix synthesis in wound healing. The precise mechanism, however, in molecular and gene expression levels still remains partially understood. Using cDNA microarray technology, the alternation of genes expression profiles was determined in a human dermal fibroblast in vitro in the presence of asiaticoside (30 microg/ml). Fifty-four genes, with known functions for cell proliferation, cell-cycle progression and synthesis of the extracellular matrix, were significantly up-regulated in our "whole-genes nest" expression profile at various timepoints. Furthermore, mRNA levels and protein productions of certain genes responsible for extracellular matrix (ECM) synthesis (e.g. encoding type I and type III collagen proteins) were evaluated by Northern blot and radioimmunoassay, respectively. As a result, there is a close correlation among the gene profile, mRNA and protein production in the cells response to asiaticoside stimulation. This information could be used for exploring the target genes in response to asiaticoside in fibroblasts.

journal_name

Int J Dermatol

authors

Lu L,Ying K,Wei S,Fang Y,Liu Y,Lin H,Ma L,Mao Y

doi

10.1111/j.1365-4632.2004.02047.x

subject

Has Abstract

pub_date

2004-11-01 00:00:00

pages

801-7

issue

11

eissn

0011-9059

issn

1365-4632

pii

IJD2047

journal_volume

43

pub_type

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