当前位置： SCI文献检索 > VACCINE期刊下所有文献 > Immunisation against plague by transcutaneous and intradermal application of subunit antigens.

Immunisation against plague by transcutaneous and intradermal application of subunit antigens.

Abstract：

:We have investigated immunological responses in BALB/c mice following transcutaneous (TC) delivery of fraction 1 (F1) and V subunits from Yersinia pestis in conjunction with an enterotoxin-derived adjuvant (cholera toxin, CT). It was found that two or more TC applications of F1 and V subunits (admixed with cholera toxin) served to elicit significant levels of anti-F1 and V antibodies in the serum of immunised mice. IL-6 secretion from cultured splenocytes derived from immunised mice indicated that a single TC application of F1 and V subunits (admixed with cholera toxin) conferred a cell-mediated response. As compared with intranasal or direct intradermal injection of F1 and V, the numbers of F1/V-specific antibody-forming cells in the spleens of animals immunised by TC application of F1 and V (admixed with CT) was relatively low. It was noted that TC application of F1 and V admixed with CT was very effective for priming responses that were boosted by intranasal or intradermal routes. Similarly, it was found that TC application of F1 and V admixed with CT could be used to efficiently boost pre-existing responses engendered by intradermal injection or intranasal instillation of F1 and V. In order to assess if TC application of F1 and V admixed with CT could protect experimental animals from plague, immunised mice were injected with a virulent strain of Y. pestis. It was found that two TC applications of F1 and V admixed with CT conferred only limited protection against 10(2) MLDs. However, three TC applications of F1 and V admixed with CT conferred solid protection against 10(2) MLDs. Hence we have shown, for the first time, that TC application of F1 and V admixed with CT can protect animals against challenge with a virulent strain of plague causing bacteria. These data suggest that transcutaneous immunisation may be a simple and non-invasive method for immunising individuals against plague.

journal_name

Vaccine

journal_title

Vaccine

authors

Eyles JE,Elvin SJ,Westwood A,Lebutt CS,Alpar HO,Somavarapu S,Williamson ED

doi

10.1016/j.vaccine.2004.02.049

subject

Has Abstract

pub_date

2004-10-22 00:00:00

pages

4365-73

issue

31-32

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(04)00406-2

journal_volume

pub_type

杂志文章

在线工具

Immunisation against plague by transcutaneous and intradermal application of subunit antigens.