A study of host preference in tsetse flies using a modified heteroduplex PCR-based method.

Abstract:

:A study of host preference in tsetse flies using a modified heteroduplex PCR-based method is described. Domestic and wild animal blood samples were collected to extract the corresponding reference DNAs. In Campo (south Cameroon), tsetse flies (mainly Glossina palpalis palpalis) were trapped and 41 bloodmeals were collected. All reference DNAs and 37 bloodmeal DNAs (90.7%) were successfully amplified and hybridised. Twelve bloodmeals (32.4%) were of human origin, 13 (35.4%) were from Sitatunga (Tragelaphus spekei) (an antelope) while 12 (32.4%) were not identified using our set of reference DNAs. The results confirmed the occurrence of frequent contacts between wild animals and this population of tsetse flies.

journal_name

Acta Trop

journal_title

Acta tropica

authors

Njiokou F,Simo G,Mbida Mbida A,Truc P,Cuny G,Herder S

doi

10.1016/j.actatropica.2004.03.006

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

117-20

issue

2

eissn

0001-706X

issn

1873-6254

pii

S0001706X04000671

journal_volume

91

pub_type

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