Identification of the resistance of a novel molecule heat shock protein 90alpha (HSP90alpha) in Microtus fortis to Schistosoma japonicum infection.

Abstract:

:Microtus fortis is a naturally resistant vertebrate host of Schistosoma japonicum by preventing completion of parasite's life cycle. Sera of M. fortis were found to have anti-schistosome effect in vitro and in vivo. In order to identify genes associated with the anti-schistosome effect of M. fortis, we screened a M. fortis marrow cDNA expression library by expression cloning and identified a 331-bp clone gC14.75. It was the homologue of heat shock protein 90alpha (HSP90alpha). Full-length of M. fortis HSP90alpha gene, Mf-HSP90alpha, was amplified according to gC14.75 and Cricetulus griseus HSP90alpha. To test the potential anti-schistosome function of Mf-HSP90alpha, we prepared conditioned medium of Mf-HSP90alpha and added it to schistosomula cultured in vitro. It caused 27.0% schistosomula death rate in 96h, which was considerably higher than that of negative control. We transferred Mf-HSP90alpha by retroviral expression vector pLXSN into mice to investigate its anti-schistosome effect in vivo. Compared with those of DMEM injection control, mice injected with Mf-HSP90alpha recombinant retrovirus had 40.8% worm burden reduction and 57.9% reduction in liver eggs per gram (LEPG) indicating its anti-schistosome effect in vivo. Taken together, our results suggested Mf-HSP90alpha as a novel anti-schistosome molecule in vitro and in vivo.

journal_name

Acta Trop

journal_title

Acta tropica

authors

Gong Q,Cheng G,Qin ZQ,Xiong DH,Yu YJ,Zeng QR,Hu WX

doi

10.1016/j.actatropica.2010.03.007

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

220-6

issue

3

eissn

0001-706X

issn

1873-6254

pii

S0001-706X(10)00085-9

journal_volume

115

pub_type

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