Abstract：
:Plasmodium falciparum transcripts contain long untranslated regions (UTR), with some of the longest in any eukaryote that uses monocistronic transcription. Owing to the extreme AT nucleotide bias within the intergenic regions that encode these UTR, attempts to characterise how they are apportioned over genes and to describe their contribution to the absolute and temporal control of gene expression have been limited. Here we describe a study using a typical house-keeping gene that encodes phosphoglutamase 2 (PFD0660w), whose expression is subject to developmentally linked control during intraerythrocytic development. We show that deletion of a significant proportion (80%) of the predicted 5' UTR has no apparent effect on the developmentally linked expression of a luciferase reporter cassette. Further, serial deletions reveal that whilst the absolute level of transcription is unaffected when up to 50% of the predicted 5' UTR is removed, the subsequent efficiency of translation is affected. These data provide key insights into the interplay of transcriptional and post-transcriptional mechanisms in the control of gene expression in this important human pathogen.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Hasenkamp S,Russell K,Ullah I,Horrocks Pdoi
10.1016/j.actatropica.2013.03.007subject
Has Abstractpub_date
2013-07-01 00:00:00pages
69-74issue
1eissn
0001-706Xissn
1873-6254pii
S0001-706X(13)00075-2journal_volume
127pub_type
杂志文章相关文献
ACTA TROPICA文献大全abstract：:In July 1989, blood samples were collected from parasitaemic cattle in the Ghibe valley, Ethiopia, frozen in liquid nitrogen and transported to Nairobi, Kenya. Twelve of the stabilates were inoculated into individual Boran (Bos indicus) calves and characterised for their sensitivity, in turn, to diminazene aceturate (...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/0001-706x(93)90026-8
更新日期：1993-04-01 00:00:00
abstract：:A key tool for the control, elimination, and eradication of Plasmodium vivax is the development of an effective vaccine. The thrombospondin-related adhesion protein (TRAP) is one of the major sporozoite antigens that plays an important role in the invasion of mosquito salivary glands and hepatocytes by sporozoites. Th...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.05.026
更新日期：2017-09-01 00:00:00
abstract：:The infection rates and development of Leishmania chagasi in two sandfly species, Lutzomyia evansi and Lutzomyia longipalpis, were evaluated under natural and experimental conditions. Natural infection rates of Lu. evansi in San Andrés de Sotavento (Colombia) and Montañas de Peraza (Venezuela) (0.05 and 0.2%, respecti...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(02)00189-4
更新日期：2003-01-01 00:00:00
abstract：:Four-day-old epimastigote culture forms of Trypanosoma cruzi, Trypanosoma rangeli and Trypanosoma conorhini were tested with 21 lectins. Furthermore T. conorhini was incubated with the following sera: rat, Wistar HAN, germ free; normal fresh hen, rat and human serum. T. rangeli was agglutinated only by the D-mannose s...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:Microtus fortis is a naturally resistant vertebrate host of Schistosoma japonicum by preventing completion of parasite's life cycle. Sera of M. fortis were found to have anti-schistosome effect in vitro and in vivo. In order to identify genes associated with the anti-schistosome effect of M. fortis, we screened a M. f...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2010.03.007
更新日期：2010-09-01 00:00:00
abstract：:Visceral leishmaniasis (VL) is caused by the trypanosomatid parasite Leishmania infantum (=Leishmania chagasi), and is epidemiologically relevant due to its wide geographic distribution, the number of annual cases reported and the increase in its co-infection with HIV. Two vector species have been incriminated in the ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2013.08.014
更新日期：2014-01-01 00:00:00
abstract：:The aim of this study was to develop transfersomal formulation with respect to dermal delivery of paromomycin sulfate (PM) for possible topical therapy of cutaneous leishmaniasis (CL). PM transfersomal formulations (PMTFs) with different percent of soy phosphatidylcholine, sodium cholate (Na-Ch) and ethanol were prepa...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2012.06.004
更新日期：2012-10-01 00:00:00
abstract：:Anti-T. dionisii and anti-T. vespertilionis monoclonal antibodies secreted by 17 hybridoma clones were tested against various strains of T. dionisii, T. vespertilionis, T. cruzi and T. cruzi marinkellei. Strain and species specific antigens were detected for the homologous immunizing strains. The common antigenic dete...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:Numerical alterations of circulating lymphocytes were investigated in 37 Brazilian patients with uncomplicated Plasmodium falciparum malaria and in a group of 15 healthy controls. The number of CD4+ T helper/inducer cells was significantly lower in patients than controls, whereas absolute numbers of CD8+ suppressor/cy...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/0001-706x(89)90047-8
更新日期：1989-10-01 00:00:00
abstract：:As strictly haematophagous insects, tsetse flies feed on a wide variety of wild and domestic animals. Although these are mainly mammals, some tsetse species also feed on reptiles. The present study investigated whether the odours of several potential natural tsetse hosts may be used as novel attractants to improve the...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(97)00083-1
更新日期：1997-11-01 00:00:00
abstract：:Human cystic echinococcosis (CE) has been considered to be caused predominantly by Echinococcus granulosus sensu stricto (the dog-sheep strain). Molecular approaches on CE, however, have revealed that human cases are also commonly caused by another species, Echinococcus canadensis. All indices for classification and s...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2016.05.013
更新日期：2017-01-01 00:00:00
abstract：:Glugea eda n. sp. is described from the mesenteries of the striated fusilier, Caesio striata, collected from the Red Sea coast off Yanbu' al Bahr, Saudi Arabia. Numerous blackish xenomas, ranged from 3 to 5 mm, were found in the body cavity associated with the mesenteries. Mature spores are monomorphic, ellipsoidal wi...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2020.105331
更新日期：2020-04-01 00:00:00
abstract：:We recently reported that BmAFI, an anti-inflammatory fraction of Brugia malayi adult worm supports parasite development in the hostile peritoneal cavity (p.c.) of Mastomys coucha through a modified Th2 type of response that includes IL-13 and IgE response and anti-inflammatory IL-10 cytokine milieu. In the present st...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2013.04.006
更新日期：2013-08-01 00:00:00
abstract：:The drawback of existing techniques for obtaining intact granulomas and granuloma cells have been, (1) lack of clean intact granulomas without adhering liver tissues, (2) isolating inadequate numbers of clean intact granulomas, and (3) obtaining low numbers of viable granuloma cells from the granulomas. This work aims...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(98)00034-5
更新日期：1998-06-30 00:00:00
abstract：:The envelope (E) protein from DENV, contain three functional and structural domains (DI, DII and DIII). Some studies suggest that neutralizing antibodies during natural DENV infection are predominantly against DI and DII, in contrast, low proportion of the antibodies were against DIII. Thus it is necessary to establis...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.04.006
更新日期：2017-07-01 00:00:00
abstract：:India and other Southeast Asian countries are severely affected by Japanese encephalitis (JE), one of the deadliest vector-borne disease threat to human health. Several epidemiological observations suggest climate variables play a role in providing a favorable environment for mosquito development and virus transmissio...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2020.105701
更新日期：2020-12-01 00:00:00
abstract：:Cystic echinococcosis (CE) is a worldwide zoonosis caused by the Echinococcus granulosus larval stage. The currently available therapy for this disease is based on benzimidazoles, which are rarely curative and cause several adverse effects. Therefore, new treatment options are needed. Octreotide (Oct) is a somatostati...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2019.105312
更新日期：2020-03-01 00:00:00
abstract：:This review paper examines the evidence from published and previously unpublished sources for the efficacy and safety of albendazole (ABZ) obtained in the last 12 years. Benzimidazole treatment alone is not ideal, requiring prolonged administration over many weeks, with an unpredictable outcome in terms of response ra...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/s0001-706x(96)00640-7
更新日期：1997-04-01 00:00:00
abstract：:Domestic dogs and cats are major domestic reservoir hosts of Trypanosoma cruzi and a risk factor for parasite transmission. In this study we assessed the relative performance of a polymerase chain reaction assay targeted to minicircle DNA (kDNA-PCR) in reference to conventional serological tests, a rapid dipstick test...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2013.03.001
更新日期：2013-06-01 00:00:00
abstract：:Live bird market (LBM) is integral component in the perpetuation of HPAI H5N1, while biosecurity is crucial and key to the prevention and control of infectious diseases. Biosecurity compliance level and risk factor assessments in 155LBMs was evaluated in Nigeria and Egypt through the administration of a 68-item biosec...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2016.08.030
更新日期：2016-12-01 00:00:00
abstract：:Malaria is transmitted by Plasmodium parasites through the bite of female Anopheles mosquitoes. One of the most important mosquito vectors in the Greater Mekong Subregion is Anopheles dirus. This study reports RNA sequencing (RNA-Seq) transcriptome analysis of An. dirus at 18 hours and 7 days after a P. vivax-infected...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2020.105502
更新日期：2020-07-01 00:00:00
abstract：:Resistance to chloroquine (CQ) in Plasmodium falciparum has a major impact on malaria control worldwide. To gain insight into early parasite stress response, mRNA expression profiles were determined for a set of 10 antioxidant defence genes in synchronized CQ-sensitive (3D7) and CQ-resistant (Dd2) clones under transie...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2010.01.013
更新日期：2010-05-01 00:00:00
abstract：:Plasmodium vivax merozoite surface protein 1 (PvMSP-1) is a leading malaria vaccine candidate. This protein is processed to give rise to various sized fragments during merozoite maturation. Here, we describe the analysis of genetic diversity in the 42 kDa C-terminal part of this protein among 33 Indian P. vivax isolat...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2008.08.011
更新日期：2008-10-01 00:00:00
abstract：:Dipterans, especially the sarcosaprophagous communities are of substantial importance from medical, veterinary and forensic entomological perspectives. Muscids are generally seen to colonize carcasses at advanced stages of decomposition when the initial dominance of calliphorids and sarcophagids subsides. Ophyra capen...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2018.06.023
更新日期：2018-09-01 00:00:00
abstract：:Global demand for food is surging upward due to the expansion of human population. Hence food production and supply need to double with little or no waste to meet this demand. Food loss at any point in the farm to fork chain inhibits the stability of food security at household and national level. A retrospective (AR) ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.12.007
更新日期：2018-02-01 00:00:00
abstract：:Manipulating the endogenous immune responses of the mosquito such as temporal and spatial expression of antimicrobial peptides may help in the development of a refractory mosquito, unable to transmit malaria. In mosquito several small antimicrobial peptides are activated locally in the midgut and salivary glands upon ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2008.01.001
更新日期：2008-04-01 00:00:00
abstract：:Antimicrobial resistance results in selective colonization in animals. In the present study, 447 diarrheic foals (235 horse foals, 165 donkey foals and 47 mule foal) were selected from Lahore and Sahiwal districts of Punjab, Pakistan. Fresh fecal and blood samples from diarrheic foals were collected for isolation and ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.08.003
更新日期：2017-12-01 00:00:00
abstract：:The study aimed to establish if there was any relationship between the blood group of the human host and schistosomiasis prevalence, intensity, incidence and related organ pathology. Urine and stool specimens were collected from the 735 school children attending a rural school in Zimbabwe to determine the Schistosoma ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(97)00671-2
更新日期：1997-05-30 00:00:00
abstract：:One of the leading brain disorders in developing countries is represented by epilepsy. It is estimated that 80% of people suffering from epilepsy around the world, reside in developing world such as Africa. Many perinatal and postnatal causes are brain-stressers in people suffering from malnutrition and low economical...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/s0001-706x(03)00038-x
更新日期：2003-06-01 00:00:00
abstract：:Chandipura virus (CHPV) is an arthropod borne rhabdovirus associated with acute encephalitis in children below the age of 15 years in the tropical states of India. Although the entry of the virus into the nervous system is among the crucial events in the pathogenesis of CHPV, the exact mechanism allowing CHPV to invad...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2014.03.028
更新日期：2014-07-01 00:00:00
