Abstract：
:Malaria is transmitted by Plasmodium parasites through the bite of female Anopheles mosquitoes. One of the most important mosquito vectors in the Greater Mekong Subregion is Anopheles dirus. This study reports RNA sequencing (RNA-Seq) transcriptome analysis of An. dirus at 18 hours and 7 days after a P. vivax-infected blood meal, which represent infection at the ookinete and oocyst parasite developmental stages, respectively. Following infection, 582 An. dirus transcripts were modulated. The 2,408 P. vivax transcripts could be classified into ookinete-specific, two-stage, and oocyst-specific groups. Results were validated by quantitative reverse transcription polymerase chain reaction. Gene ontology analysis of the vector and parasite revealed several biological pathways for both, providing a better understanding of Anopheles-Plasmodium interactions at the ookinete and oocyst stages.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Boonkaew T,Mongkol W,Prasert S,Paochan P,Yoneda S,Nguitragool W,Kumpitak C,Sattabongkot J,Kubera Adoi
10.1016/j.actatropica.2020.105502subject
Has Abstractpub_date
2020-07-01 00:00:00pages
105502eissn
0001-706Xissn
1873-6254pii
S0001-706X(20)30059-0journal_volume
207pub_type
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