Spatial distribution and molecular characterization of Anopheles nivipes and Anopheles philippinensis (Diptera: Culicidae) in north-east India.

Abstract:

:Anopheles philippinensis-nivipes species of mosquitoes are considered secondary malaria vectors in the north-east India. However, difficulty in separating An. philippinensis and An. nivipes accurately on the basis of morphological identification, due to the overlapping female wing characters, has led to the paucity of information on their spatial distribution and vectorial importance, essential for understanding malaria disease epidemiology in areas under their influence. In view of this a study was undertaken to generate authentic information on distribution of An. philippinensis and An. nivipes in the north-east India using molecular tools. Between the two, An. nivipes was recorded as the dominant species in Assam, and Nagaland states whereas An. philippinensis was found predominant in the state of Mizoram and Arunachal Pradesh. Species differentiation based on female wing characters proved wrong in about 74% specimens of An. philippinensis but found accurate in case of An. nivipes. No intra-specific variation in second internal transcribed spacer (ITS2) region of ribosomal DNA(r-DNA) in either An. nivipes or An. philippinensis from different geographical areas of north-east India was noted.

journal_name

Acta Trop

journal_title

Acta tropica

authors

Sarma NP,Prakash A,Bhattacharyya DR,Kalita MC,Mohapatra PK,Singh S,Sarma DK,Mahanta J

doi

10.1016/j.actatropica.2012.02.065

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

247-54

issue

3

eissn

0001-706X

issn

1873-6254

pii

S0001-706X(12)00122-2

journal_volume

122

pub_type

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