Abstract：
:Free Living Amoebae (FLA) of Acanthamoeba genus are widely distributed in the environment and can be found in the air, soil and water; and have also been isolated from air-conditioning units. In humans, they are causative agents of a sight-threating infection of the cornea, Acanthamoeba keratitis (AK) and a fatal infection of the central nervous system known as Granulomatous Amoebic Encephalitis (GAE). In this study, a survey was conducted in order to determine the presence and pathogenic potential of free-living amoebae of Acanthamoeba genus in nasal swabs from individuals in two regions of Peru. Identification of isolates was based on cyst morphology and PCR-sequencing of the Diagnostic Fragment 3 to identify strains at the genotype level. The pathogenic potential of the isolates was also assayed using temperature and osmotolerance assays and extracellular proteases zymograms. The obtained results revealed that all isolated strains exhibited pathogenic potential. After sequencing the highly variable DF3 (Diagnostic Fragment 3) region in the 18S rRNA gene as previously described, genotype T4 was found to be the most common one in the samples included in this study but also genotype T15 was identified. To the best of our knowledge, this is the first study on the characterization of Acanthamoeba strains at the genotype level and the first report of genotype T4 and T15 in Peru.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Cabello-Vílchez AM,Martín-Navarro CM,López-Arencibia A,Reyes-Batlle M,González AC,Guerra H,Gotuzzo E,Valladares B,Piñero JE,Lorenzo-Morales Jdoi
10.1016/j.actatropica.2013.10.006subject
Has Abstractpub_date
2014-02-01 00:00:00pages
7-10eissn
0001-706Xissn
1873-6254pii
S0001-706X(13)00280-5journal_volume
130pub_type
杂志文章相关文献
ACTA TROPICA文献大全abstract：:Bovine cryptosporidiosis is mainly caused by four distinct species: Cryptosporidium parvum, C. bovis, C. ryanae and C. andersoni. The first, C. parvum, is a major concern in livestock causing economic losses, in addition to public health impact because of its zoonotic characteristics. The present study aimed to determ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2013.11.002
更新日期：2014-02-01 00:00:00
abstract：:Schistosomiasis is one of the most common parasitic diseases in tropical and subtropical areas, including Brazil. A national control programme was initiated in Brazil in the mid-1970s and proved successful in terms of morbidity control, as the number of cases with hepato-splenic involvement was reduced significantly. ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2013.12.007
更新日期：2014-04-01 00:00:00
abstract：:Swine influenza is responsible for one of the most prevalent disease affecting the swine industry worldwide. Epidemiological surveys rarely focus on remote areas, because traditional farming systems characterized by locally consumed production and low pig densities are considered as having little influence on the emer...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2011.11.012
更新日期：2012-04-01 00:00:00
abstract：:This study evaluated plant-based immune-adjuvants from crude extracts of Phoenix dactylifera and Mentha piperita as promising adjuvants for vaccines because of the limited side effects associated with plant extracts. In addition, Montanide™ ISA 201 previously used in vaccines in cattle. Eight different infectious cory...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2019.105281
更新日期：2020-02-01 00:00:00
abstract：:The role of phospholipids (PLs) in the signal transduction pathways that are activated by a mitogenic stimulus (foetal calf serum) in Trypanosoma cruzi epimastigotes (EPI) was investigated. Only phosphatidylinositol-bis-phosphate was significantly altered in this process. Other phosphoinositides, including major PLs s...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(99)00016-9
更新日期：1999-07-30 00:00:00
abstract：:Primaquine has been the drug of choice for the prevention of Plasmodium vivax relapse for more than 60 years. Primaquine tolerant strain of P. vivax was identified in 1944. Significant mortality and disease burden of P. vivax calls for the need of new drugs. Primaquine resistance is a complex issue, as the mechanism o...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2016.04.009
更新日期：2016-08-01 00:00:00
abstract：:Giardiasis is an intestinal parasitosis that affects millions of people worldwide and is considered a zoonotic disease. Frequently in contact with humans, dogs are the main host involved in this zoonotic transmission. Here, we compared some aspects of Giardia duodenalis biology between two strains: a recently isolated...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.07.002
更新日期：2017-10-01 00:00:00
abstract：:Currently, benzimidazoles are used as chemotherapeutic agents and as a complement to surgery and PAIR in the treatment of cystic echinococcosis (CE). They are generally applied at high doses causing side effects and, 50% of cases do not respond favorably to such chemotherapy. The use of essential oils obtained by dist...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2016.09.001
更新日期：2016-12-01 00:00:00
abstract：:Greece has been recently affected by several mosquito borne diseases with the West Nile Virus (WNV) outbreak in 2010 being one of the largest reported in Europe. Currently at the epicenter of an economic and refugee crisis and visited by over 16 million tourists a year the integrated management of diseases transmitted...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.06.005
更新日期：2017-10-01 00:00:00
abstract：:Two high-throughput assays for the identification of members of the Anopheles gambiae sensu lato species complex have recently been reported. These methods, are based on TaqMan single nucleotide polymorphism (SNP) genotyping that enables rapid scoring of mosquito DNA samples in real-time PCR reactions. Unfortunately, ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2008.04.009
更新日期：2008-07-01 00:00:00
abstract：:Two PCR methods were compared for their sensitivity in detecting cultured Leishmania major, before being used to estimate infection rates in female sandflies (Phlebotomus papatasi) collected from peridomestic animal shelters and the nearby burrows of the gerbil reservoir hosts, Rhombomys opimus, in Isfahan province, c...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2004.09.007
更新日期：2005-01-01 00:00:00
abstract：:Glutathione transferases (GSTs) perform several catalytic and non-catalytic roles in the defense against toxicities of electrophile compounds and oxidative stress, and therefore are involved in stress-response and cell detoxification. Previously, we have provided evidence indicating that EgGST2 and EgGST3, two phyloge...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2018.01.007
更新日期：2018-04-01 00:00:00
abstract：:A study was conducted to determine the presence of hemagglutination inhibition (HI) and neutralization antibodies against Japanese encephalitis virus (JEV) in wild pigs on an offshore island in Singapore. Blood samples were collected from 28 wild pigs on the island. All the sera tested with HI assay and plaque reducti...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(01)00212-1
更新日期：2002-03-01 00:00:00
abstract：:Chagas disease, caused by Trypanosoma cruzi affects about 6-8 million people worldwide. Although transmission by triatomine insects has been controlled, other means of transmission maintain the infection. These forms of transmission are responsible for introducing Chagas disease in other non-endemic countries of the w...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.08.025
更新日期：2017-12-01 00:00:00
abstract：:We present data showing that structures serving as drains and catch basins for stormwater are important sources for production of the mosquito arbovirus vectors Aedes aegypti and Culex quinquefasciatus in Mérida City, México. We examined 1761 stormwater drains - located in 45 different neighborhoods spread across the ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2014.01.011
更新日期：2014-06-01 00:00:00
abstract：:Alpha-amylase and alpha-glucosidase activities were found in homogenates of young, unfed male and female Phlebotomus papatasi and in gut and salivary gland preparations. A significant increase in both enzyme activities in females and of alpha-amylase in males was recorded for flies that had fed overnight on a plant (C...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(00)00164-9
更新日期：2001-01-15 00:00:00
abstract：:Anopheles balabacensis balabacensis and Anopheles minimus are the main malaria vectors in Thailand. In a cleared forested foothill area in the central part of the country A. minimus was the most prevalent anopheline species found, only 6 specimens of A. b. balabacensis being collected over a 3-year period. Cattle were...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1978-03-01 00:00:00
abstract：:We chose five easily propagated garden plants previously shown to be attractive to mosquitoes, ants or other insects and tested them for attractiveness to Culex pipiens and Aedes aegypti. Long term imbibition was tested by survival on each plant species. Both mosquito species survived best on Impatiens walleriana, the...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2015.03.010
更新日期：2015-06-01 00:00:00
abstract：:The population dynamics of the larval and adult life stages of the malaria vector Anopheles gambiae Giles were studied in Miwani, western Kenya, in relation to meteorological conditions. Larval density within a habitat, the number of larval habitats and sibling species composition were investigated as determinants of ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2003.11.007
更新日期：2004-04-01 00:00:00
abstract：:It has been reported a higher seroprevalence of HBV and HCV in leprosy patients than in the general population, but the reasons for these findings are not yet clear. On the other hand, there is evidence that these viruses may influence the onset of leprosy reactional episodes, an important cause of neurological sequel...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.04.024
更新日期：2017-08-01 00:00:00
abstract：:The information in this protocol covers from the basic steps and material necessary to start a sand fly colony up to the specific details which are important to the success of a Nyssomyia neivai colony. The greatest problems in our colony of Ny. neivai were solved with specific care, for instance, using vermiculite an...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.07.010
更新日期：2017-10-01 00:00:00
abstract：:Brucellosis is caused by the genus Brucella. Brucella is widely distributed in cattle, swine, sheep, goat and other mammals including human. Animal brucellosis causes severe economic losses and affects related international transportation and trade. Human brucellosis causes both acute and chronic symptoms of multi-org...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2019.05.017
更新日期：2019-09-01 00:00:00
abstract：:In this study, recombinase polymerase amplification (RPA) combined with SYBR Green I was developed for the detection of Plasmodium knowlesi. Positive samples were indicated with a green color while negative samples were orange. To increase the efficiency of amplification, an interval mixing step of samples after 3 to ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2020.105511
更新日期：2020-05-15 00:00:00
abstract：:Wuchereria bancrofti, a nematode parasite causing human lymphatic filariasis is widely distributed in India. The phylogeography of this parasite was studied by constructing RAPD profiles of parasite populations collected from 71 microfilaria carriers residing in different geoclimatic regions of India. The analysis sho...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2006.06.004
更新日期：2006-07-01 00:00:00
abstract：:A survey was undertaken between November 1994 and January 1995 to assess the knowledge and practice with respect to malaria of 1946 randomly selected households in endemic urban and rural areas of Hormozgan, Kerman and Sistan and Baluchistan Provinces, southeast Iran. Knowledge of inhabitants was relatively poor in th...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(96)00609-2
更新日期：1997-04-15 00:00:00
abstract：:Indirect fluorescent antibody (IFA) tests and enzyme-linked immunosorbent assays (ELISA) were used to measure antibodies to Plasmodium falciparum in an indigenous population in an area of Malaysia with high malaria prevalence. The results of three surveys were analyzed to examine the relation of these serologic measur...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/0001-706x(90)90054-4
更新日期：1990-12-01 00:00:00
abstract：:Echinococcosis, a disease caused by infection with the larval stage of a tapeworm parasite of the genus Echinococcus, is of major socio-economic importance, and studying genetic variability within and between Echinococcus populations has important implications for disease control and epidemiology. Various DNA approach...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(98)00052-7
更新日期：1998-09-15 00:00:00
abstract：:Angiostrongylus cantonensis is the main cause of human eosinophilic meningitis. Humans are accidental hosts, becoming infected due to ingestion of raw intermediate (snails and slugs) or paratenic hosts. Once ingested, the larvae migrate towards the brain where they die, causing the disease. To develop better mollusk c...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2020.105684
更新日期：2020-12-01 00:00:00
abstract：:The kinetic and dynamic disposition of endo- and ectoparasiticides in livestock in relation to development of resistance is examined. Based on the modes of action of antiparasitic drugs, maximum activity necessitates that the parasite be exposed to 'toxic' concentrations for as great a duration as possible. In contras...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/0001-706x(94)90059-0
更新日期：1994-03-01 00:00:00
abstract：:Representatives of the genus Trypanosoma have been traditionally found in epimastigote, espheromastigote and trypomastigote flagellated forms in axenic cultures. Trypanosoma caninum is a trypanosomatid that has recently been reported infecting dogs in endemic areas of canine leishmaniasis in Brazil. It presents specif...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2014.05.013
更新日期：2014-09-01 00:00:00
