Abstract：
:In this study, recombinase polymerase amplification (RPA) combined with SYBR Green I was developed for the detection of Plasmodium knowlesi. Positive samples were indicated with a green color while negative samples were orange. To increase the efficiency of amplification, an interval mixing step of samples after 3 to 6 min incubation was recommended. Different sets of reaction volumes from 6.25 to 50 µL were tested and the results indicated no differences in detection. RPA's combination with SYBR Green I is fast and easy to perform, hence this method is suitable for use in resource-limited settings.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Lai MY,Lau YLdoi
10.1016/j.actatropica.2020.105511subject
Has Abstractpub_date
2020-05-15 00:00:00pages
105511eissn
0001-706Xissn
1873-6254pii
S0001-706X(20)30317-Xjournal_volume
208pub_type
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